Team:Goettingen/week13-3

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<b>V07_25_2 2<sup>nd</sup> round: </b><br>
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<b>V07_25_2 2<sup>nd</sup> round: Saturated mutagenesis PCR 200 µL (repeat)</b><br>
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<li>Experiment: <br>The PCR was set up following the protocol from week <a href="https://2012.igem.org/Team:Goettingen/week10-3">10</a>. </li>
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<li>Observations and results: <br>The corresponding agarose gel showed bands of the expected sizes. </li>
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Revision as of 15:10, 24 September 2012

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#3 Chemoreceptor Library - 13th Week

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V07_24


2nd round: Analysis of plates and liquid culture V07_20
  • Experiment:
    Both the liquid culture and the plates exhibited bacterial growth. 14 clones were transferred to a 5 mL liquid culture (LB + CM) and incubated over night at 37 °C, 180 rpm. The liquid culture was centrifuged at 4800 rpm, 4 °C, 10 min. The pellet was stored at -20 °C.


V07_25


V07_25_1 2nd round: Plasmid preparation clones 1-14 for subsequent sequencing
  • Experiment:
    Plasmid preparation was performd with peqGOLD Plasmid Miniprep Kit (PeqLab) following the user manual. DNA concentrations were determined via nanodrop for subsequent sequencing.

V07_25_2 2nd round: Saturated mutagenesis PCR 200 µL (repeat)
  • Experiment:
    The PCR was set up following the protocol from week 10.

  • Observations and results:
    The corresponding agarose gel showed bands of the expected sizes.


V07_26


2nd round:
  • Experiment:


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