Team:Goettingen/week5-1

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<h2><b>VX_Y </b></h2><br>
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<h2><b>V05_31 </b></h2><br>
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<b>Titel</b><br>
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<b>V05_31_1: Chemotaxis of flhDC-upregulated <i>E.coli</i> on beef-extract plates with different Agar/Trypton concentrations</b><br>
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<li>Experiment: <br>hier text reinschreiben</li>
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<li>Experiment: Agar preparation: 0.5% Trypton and 1.0% Trypton are tested with 0.3% and 0.4% agar. Additional the agar contained 1,0% NaCl and 0.3% beef extract<br></li>
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<li>Observations & Results: <br></li>
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<h2><b>V06_01 </b></h2><br>
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<b>V06_01_1: Swimming assay with P1-18C3-DH10B</b><br>
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<li>Experiment: Different cell concentrations were tested on different agar compositions<br></li>
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<li>Observations & Results: Most of the bacteria drops did not move at all after 20.5h of incubation at 30°C.<br></li>
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<h2><b>V06_02 </b></h2><br>
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<b>V06_02_1: Swimming plates were exposed to UV light, which may induce random speed-enhancing mutations </b><br>
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<li>Experiment: Different plates were exposed for different time periods (1, 2, 5, and 10 min) to UV light. Afterwards, the plates were incubated at 30°C to identify cells with improved swimming later on.<br></li>
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<li>Observations & Results: No improved or enhanced swimming was detectable. Therefore it can be assumed that no benefitial mutations have occured.<br></li>
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Revision as of 16:17, 25 September 2012

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#1 Selection / Swimming - 5th Week

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V05_31


V05_31_1: Chemotaxis of flhDC-upregulated E.coli on beef-extract plates with different Agar/Trypton concentrations
  • Experiment: Agar preparation: 0.5% Trypton and 1.0% Trypton are tested with 0.3% and 0.4% agar. Additional the agar contained 1,0% NaCl and 0.3% beef extract
  • Observations & Results:


V06_01


V06_01_1: Swimming assay with P1-18C3-DH10B
  • Experiment: Different cell concentrations were tested on different agar compositions
  • Observations & Results: Most of the bacteria drops did not move at all after 20.5h of incubation at 30°C.


V06_02


V06_02_1: Swimming plates were exposed to UV light, which may induce random speed-enhancing mutations
  • Experiment: Different plates were exposed for different time periods (1, 2, 5, and 10 min) to UV light. Afterwards, the plates were incubated at 30°C to identify cells with improved swimming later on.
  • Observations & Results: No improved or enhanced swimming was detectable. Therefore it can be assumed that no benefitial mutations have occured.


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