Team:Goettingen/week10-3
From 2012.igem.org
(Difference between revisions)
Line 113: | Line 113: | ||
<b>V07_05_02 1<sup>st</sup> round: Transforamtion of electrocompetent cells wir the mutant plasmid mixture</b><br> | <b>V07_05_02 1<sup>st</sup> round: Transforamtion of electrocompetent cells wir the mutant plasmid mixture</b><br> | ||
<ul> | <ul> | ||
- | <li>Experiment: <br>Electrocompetent cells were prepared according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. We transformed 200 µL of cells with 10 µL of DNA. A detailed protocol for the electroporation can be found <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">here</a>. The transformed cells were transferred to 100 mL liquid LB medium and incubated over night at 37 °C, 180 rpm. We prepared three dilution plates LB+CM (10<sup>4</sup>, 10<sup>5</sup>, 10<sup>6</sup>) for counting colonies to verify whether the desired library diversity is reached. Plates were incubated over night at 37 °C aswell. | + | <li>Experiment: <br>Electrocompetent cells were prepared according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. We transformed 200 µL of cells with 10 µL of DNA. A detailed protocol for the electroporation can be found <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">here</a>. The transformed cells were transferred to 100 mL liquid LB medium + CM and incubated over night at 37 °C, 180 rpm. We prepared three dilution plates LB+CM (10<sup>4</sup>, 10<sup>5</sup>, 10<sup>6</sup>) for counting colonies to verify whether the desired library diversity is reached. Plates were incubated over night at 37 °C aswell. |
</li> | </li> | ||
</ul> | </ul> |
Revision as of 16:09, 22 September 2012
Deutsch / English |
#3 Chemoreceptor Library - 10th WeekBack to overview
Back to overview |