Team:Goettingen/week3-3

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<b>Isolation of pUC18 plasmid DNA</b><br>
<b>Isolation of pUC18 plasmid DNA</b><br>
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<li>Experiment: <br>Isolation of pUC18 from DH10B using peqGOLD MiniPrep Kit (Peqlab).
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<li>Experiment: <br>Isolation of pUC18 from DH10B using peqGOLD Miniprep Kit (Peqlab).
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</li>
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Latest revision as of 11:22, 22 September 2012

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#3 Chemoreceptor Library - 3rd Week

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V05_15


Preparing Media
  • Experiment:
    Preparation of LB medium and LB-Amp plates (recipe see here). Preparing streak plates of pUC18 DH10B on LB-Amp, incubation at 37 °C.


V05_16


Isolation of pUC18 plasmid DNA
  • Experiment:
    Isolation of pUC18 from DH10B using peqGOLD Miniprep Kit (Peqlab).


V05_18


Insertion of tar into pUC18
  • Experiment:
    Double digest of tar and pUC18 using EcoRI, PstI and Orange Buffer (ThermoScientific). Preparation of chemical competent DH10B cells for transformation.


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