Team:Goettingen/week2-2

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Revision as of 09:34, 22 September 2012

Deutsch  / English 

#2 Speed Improvement - 2nd week

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V05_08


Amplification of flhDC gene
  • Experiment:
    Using the isolated genomic DNA of E. coli (DH10B) as template, the gene flhDC was amplified by pfu polymerase according to the following protocol.



V05_10


Separation of flhDC
  • Experiment:
    The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
  • Observations & Results:
    The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.


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V05_11


Purificaton of flhDC
  • Experiment:
    The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.


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