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Team:Goettingen/week8-2
From 2012.igem.org
(Difference between revisions)
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<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | In order to gain further plasmid material of the new vector pSB1C3 was transformed into <i>E. coli</i> (DH10B) as described in the protocol. <br> | + | In order to gain further plasmid material of the new vector pSB1C3 was transformed into <i>E. coli</i> (DH10B) as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br> |
<li>Observations & Results: <br> | <li>Observations & Results: <br> | ||
The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li> | The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li> | ||
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<h2><b>V06_19 </b></h2><br> | <h2><b>V06_19 </b></h2><br> | ||
| - | <b>V06_19_1 Repetition of the chemical | + | <b>V06_19_1 Repetition of the chemical transformation of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B) </b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li> | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li> | ||
<li>Observations & Results: <br> | <li>Observations & Results: <br> | ||
| - | The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no | + | The transformation was successful.Due to the possesion of a gene encoding for <i>RFP</i> the developed colonied featured a red color. On the negative control no colonies were observed. |
</li></ul> | </li></ul> | ||
<br> | <br> | ||
| - | <b>V06_19_2 Repetition of the chemical | + | <b>V06_19_2 Repetition of the chemical transformation of the <i>araC</i>-promoter into <i>E. coli</i> (DH10B) </b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol. <br> | + | Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br> |
<li>Observations & Results: <br> | <li>Observations & Results: <br> | ||
The transformation was again not successful. Only the positive control showed any growth. </li> | The transformation was again not successful. Only the positive control showed any growth. </li> | ||
Revision as of 14:21, 18 September 2012
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