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Team:University College London/Module 5/Characterisation
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| - | IrrE is deduced to globally regulate gene expression in E.Coli, and to date, no specific assay allows for the convenient determination of IrrE expression. As such, the expression of IrrE will be determined indirectly by determining the increased salt tolerance of our cells. In order to confirm this, we will be conducting growth curve experiments. This will allow us to determine if our cell grow to a higher density in high salinity when compared to the untransformed cells. | + | IrrE is deduced to globally regulate gene expression in ''E. Coli'', and to date, no specific assay allows for the convenient determination of IrrE expression. As such, the expression of IrrE will be determined indirectly by determining the increased salt tolerance of our cells. In order to confirm this, we will be conducting growth curve experiments. This will allow us to determine if our cell grow to a higher density in high salinity when compared to the untransformed cells. |
As we will be using Lyon 2010 iGEM team's K540000 salt tolerance BioBrick for comparison, we will also attempt to replicate their results via growth rate experiments. | As we will be using Lyon 2010 iGEM team's K540000 salt tolerance BioBrick for comparison, we will also attempt to replicate their results via growth rate experiments. | ||
Revision as of 22:39, 16 September 2012
Module 5: Salt Tolerance
Description | Design | Construction | Characterisation | Modelling | Results | Conclusions
Characterisation
IrrE is deduced to globally regulate gene expression in E. Coli, and to date, no specific assay allows for the convenient determination of IrrE expression. As such, the expression of IrrE will be determined indirectly by determining the increased salt tolerance of our cells. In order to confirm this, we will be conducting growth curve experiments. This will allow us to determine if our cell grow to a higher density in high salinity when compared to the untransformed cells.
As we will be using Lyon 2010 iGEM team's K540000 salt tolerance BioBrick for comparison, we will also attempt to replicate their results via growth rate experiments.
