Team:Goettingen/week8-2

V06_18

• Experiment:
  In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol.
  
• Observations & Results:
  The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.

V06_19

• Experiment:
  Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
• Observations & Results:
  The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.

• Experiment:
  Since the last time the fact that the araC-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol.
  
• Observations & Results:
  The transformation was again not successful. Only the positive control showed any growth.

V06_20

• Experiment:
  Over night cultures were prepared for group 1 and group 3:
  
  20E-flhDC
  18M-flhDC
  18C-flhDC
  
  20E-tar
  18M-tar
  19C-tar