Team:Bielefeld-Germany/Labjournal/week3

Week 3 (05/14 - 05/20/12)

weekly seminar:

• first lab service: Robert
• our GFP, which we wanted to use for the summer school for pupils, does not work
• first competent cells have to be made: Julia S. and Robert
• Decision to buy a commercial laccase to establish the analytics and the enzyme tests
• our expose has to be translated into english: Malak
• last planning for our waver sell
• Julia S. is creating a vector for Pichia pastoris and is now looking for sequences
• The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process

Monday May 14th

Tuesday May 15th

• Team Activity Tests: For some pre test and characterization for our future laccase activity standard we ordered [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de&region=DE laccase] from Trametes versicolor. As well we had to order a substrate that the laccase could use to demonstrate its abilities. According to the literature [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de&region=DE ABTS] is a well working substrate to characterize oxidizing enzym activity. So we ordered.

Wednesday May 16th

• Team Bacterial Laccases: For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.

Thursday May 17th

• Team Modeling: Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
• Team Bacterial Laccases: Plasmid isolation of BBa_J04450 (pSB1C3 + RFP).

Friday May 18th

Saturday May 19th

Sunday May 20th

Sunday

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