Team:Goettingen/week2-3

(Difference between revisions)

Revision as of 19:48, 11 September 2012

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Amplification of tar gene

Experiment:
PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of tar and using Pfu polymerase according to this protocol.

Amplification of tar gene

Experiment:
PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used. A band at about 1.6 kb was detected. Amplification of tar was successful.

Amplification of tar gene

Experiment:
PCR clean-up using peqGOLD Gel Extraction Kit according to the manual.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <b>Amplification of <i>tar</i> gene</b><br>
 <ul>
-<li>Experiment: <br>PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of <i>tar</i> and using <i>Pfu</i> polymerase according to this <a href="LINK"> protocol.
+<li>Experiment: <br>PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of <i>tar</i> and using <i>Pfu</i> polymerase according to this <a href="https://2012.igem.org/Team:Goettingen/Project/Methods"> protocol.
 </li>
 </ul>