Team:HokkaidoU Japan/Notebook/plastic Week 6

From 2012.igem.org

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===August 6th===
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====Polymer extract====
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@Taguchi lab
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#sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
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#sample2 JM109 culture from July 18th 1.4642 g , 0.0040
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#sample3 DH5a                        1.4666 g , 0.0056
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====Preculture====
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Inoculated from three different plates (Name it 1,2,3).<br />
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Cultured in media of LB 150 ul + 1.5 ul of Amp.<br />
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30C  August 6th 20:00 ~ 7th 10:00<br />
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==August 6th==
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===August 7th===
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==polymer extract==
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====Liquid culture====
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We made 8 samples to see if Panthotenic acid is necessary for plastic growth. <br />
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*Panthotenic acid (+)1.5 ml 4 samples
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*Panthotenic acid (+)0.7 ml 2 samples
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*Panthotenic acid (-)0.7 ml 2 samples
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Incubated liquid culture from August 7th 11:00 until 9th 09:50.<br />
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===August 9th===
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@Taguchi lab
@Taguchi lab
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====Harvest====
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We did the protocol of harvest on 8 samples.
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</div></div>
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===August 10th===
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<div class="hokkaidou-section">
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====Plasmid extraction====
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The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.
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===August 11th===
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<div class="hokkaidou-section">
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====Sequencing====
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BBa_K342001(PhaC) was seqenced.<br />
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#sample1 JM109 culture from July18th 1.4643g , 0.0054 (tube+polymer , polymer)
+
<br style="line-height: 0; clear: both;" />
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#sample2 JM109 culture from July18th 1.4642g , 0.0040
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</div></div>
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#sample3 DH5a                        1.4666g , 0.0056
+
-
==preculture==
 
-
 
 
-
Inoculate from three different plates(Name it 1,2,3)<br />
 
-
Culture in media of LB 150microL+Amp1.5microL<br />
 
-
30℃ 20:00-7th10:00<br />
 
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</p>
 
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Latest revision as of 02:58, 27 September 2012

Contents

August 6th

Polymer extract

@Taguchi lab

  1. sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
  2. sample2 JM109 culture from July 18th 1.4642 g , 0.0040
  3. sample3 DH5a 1.4666 g , 0.0056

Preculture

Inoculated from three different plates (Name it 1,2,3).
Cultured in media of LB 150 ul + 1.5 ul of Amp.
30C August 6th 20:00 ~ 7th 10:00


August 7th

Liquid culture

We made 8 samples to see if Panthotenic acid is necessary for plastic growth.

  • Panthotenic acid (+)1.5 ml 4 samples
  • Panthotenic acid (+)0.7 ml 2 samples
  • Panthotenic acid (-)0.7 ml 2 samples

Incubated liquid culture from August 7th 11:00 until 9th 09:50.


August 9th

@Taguchi lab

Harvest

We did the protocol of harvest on 8 samples.


August 10th

Plasmid extraction

The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.


August 11th

Sequencing

BBa_K342001(PhaC) was seqenced.



Retrieved from "http://2012.igem.org/Team:HokkaidoU_Japan/Notebook/plastic_Week_6"