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Team:University College London/Protocols/Nanodrop
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| - | {{:Team:University_College_London/templates/head|coverpicture=week4}} | + | <noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} |
| - | == Nanodrop == | + | == Nanodrop ==</noinclude> |
Software ND-1000 | Software ND-1000 | ||
Model: | Model: | ||
| - | Step 1: Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software | + | '''Step 1:''' Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software |
| - | Step 2: Wipe and add elution buffer as negative control. Click blank in ND-1000 software | + | '''Step 2:''' Wipe and add elution buffer as negative control. Click blank in ND-1000 software |
| - | Step 3: Wipe and add 1 µl sample | + | '''Step 3:''' Wipe and add 1 µl sample |
| - | Step 4: On the software set lambda to 260nm | + | '''Step 4:''' On the software set lambda to 260nm |
| - | Step 5: Lower the lever arm and click measure in ND-1000 software | + | '''Step 5:''' Lower the lever arm and click measure in ND-1000 software |
| - | Step 6: Take readings for concentration and purity | + | '''Step 6:''' Take readings for concentration and purity |
| - | Step 7: Once measurement complete, wipe surface | + | '''Step 7:''' Once measurement complete, wipe surface |
| - | {{:Team:University_College_London/templates/foot}} | + | <noinclude>{{:Team:University_College_London/templates/foot}}</noinclude> |
Latest revision as of 17:28, 2 August 2012
Nanodrop
Software ND-1000 Model:
Step 1: Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software
Step 2: Wipe and add elution buffer as negative control. Click blank in ND-1000 software
Step 3: Wipe and add 1 µl sample
Step 4: On the software set lambda to 260nm
Step 5: Lower the lever arm and click measure in ND-1000 software
Step 6: Take readings for concentration and purity
Step 7: Once measurement complete, wipe surface
