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Team:University College London/Protocols/ColPic
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Rwilkinson (Talk | contribs) (Created page with "<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} == Picking Colonies == Step 1 – Creating culture media: In a sterile environment, set up X nu...") |
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<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} | <noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} | ||
| - | == Picking Colonies == | + | == Picking Colonies ==</noinclude> |
| + | '''Step 1 – Creating culture media:''' In a sterile environment, set up X numbers of falcons, each with 5mls of media. | ||
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| + | '''Step 2 - Inoculating Colonies into a Selective Broth::''' Add Yul of antibiotic to reach desired antibiotic concentration. | ||
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(For Ampicillin this is 50ug/ml, For Kanamycin it is 25ug/ml, for Tetracycline it is 15ug/ml, and for Chloramphenicol it is 25ug/ml) | (For Ampicillin this is 50ug/ml, For Kanamycin it is 25ug/ml, for Tetracycline it is 15ug/ml, and for Chloramphenicol it is 25ug/ml) | ||
| - | Step 4 – Selecting a Colony: Select a clear, isolated colony and using an inoculation hoop scoop up a colony onto the tip. Deposit in the falcon tube | + | |
| - | Step 5 - Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours. | + | |
| + | '''Step 4 – Selecting a Colony:''' Select a clear, isolated colony and using an inoculation hoop scoop up a colony onto the tip. Deposit in the falcon tube | ||
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| + | '''Step 5 - Culture:''' Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours. | ||
<noinclude> | <noinclude> | ||
{{:Team:University_College_London/templates/foot}} | {{:Team:University_College_London/templates/foot}} | ||
</noinclude> | </noinclude> | ||
Latest revision as of 15:21, 30 August 2012
Picking Colonies
Step 1 – Creating culture media: In a sterile environment, set up X numbers of falcons, each with 5mls of media.
Step 2 - Inoculating Colonies into a Selective Broth:: Add Yul of antibiotic to reach desired antibiotic concentration.
(For Ampicillin this is 50ug/ml, For Kanamycin it is 25ug/ml, for Tetracycline it is 15ug/ml, and for Chloramphenicol it is 25ug/ml)
Step 4 – Selecting a Colony: Select a clear, isolated colony and using an inoculation hoop scoop up a colony onto the tip. Deposit in the falcon tube
Step 5 - Culture: Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours.
