Team:HokkaidoU Japan

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<div id="hokkaidou-team-logo"><html><img src="https://static.igem.org/mediawiki/2012/9/9e/Hokkaidou_logo_transparent.png" height=250px width=250px alt="hokkaidou team logo" /></html></div>
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  <h2><span class="mw-headline" id="Bio-capsule_E._coli">Bio-capsule E. coli</span></h2>
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    Spherical E. coli cluster that can accumulate macromolecules in it</span></h3>
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        We have designed two modules to make E. coli implementing the function as bio-capsules that accumulate bio-plastic.
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      <p id="hokkaidou-index-cross1">
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        First module is to make bio-capsules formed by aggregation of E. coli through cell-cell interaction via adhesion molecule, Ag43,
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        located on the surface of outer membrane. The E. coli capsules can be easily collected by simple filtration without centrifuge steps.
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        Removal of centrifuge steps largely contributes for reduction of time-consuming steps in industrial production of valuable material in E. coli.
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      <p id="hokkaidou-index-cross2">
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        Second module is for producing bio-plastic (poly-3-hydroxybutyrate, P3HB). PHB is produced by microorganisms (like Ralstonia eutrophus).
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        The polymer is primarily a product of carbon assimilation from glucose and is important for microorganisms as an energy storage molecule.
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        Development of a cost-effective method for manufacturing biodegradable plastic is one of important issue for making sustainable future society.
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        We have optimized culture conditions for efficient production of E. coli bio-capsules and efficient production of P3HB
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        (first success of bio-plastic production as iGEM team). Then we will try to extend the applicability of this system for
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        producing other high-value added macromolecules in the capsule.
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      <img src="/wiki/images/7/7f/HokkaidoU2012_Biocapsule_1.png" />
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      <img src="/wiki/images/0/07/HokkaidoU2012_Biocapsule_3.png" />
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==Bio-capsule E. coli==
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  <h2 style="text-align: right;"><span class="mw-headline" id="Biobrick_Reviews">Biobrick Reviews</span></h2>
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===Spherical E. coli cluster that can accumulate macromolecules in it===
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    <div class="hokkaidou-index-image">
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      <img src="/wiki/images/d/d7/Hokkaidou2012_Index_Human_Practice.png" alt="human practice" />
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We have designed three modules to make E. coli implementing the function as a bio-capsule.  First module is for producing high-value added macromolecules.  Second module is for aggregation of E. coli by cell-cell interaction through adhesion molecule located on the surface of outer membrane. Third module is for collecting produced materials to the central space of the capsule.
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This year, we will try to make bio-plastic (poly-3-hydroxybutyrate, P3HB) producer for showing first example of bio-capsule. PHB is produced by microorganisms (like Ralstonia eutrophus). The polymer is primarily a product of carbon assimilation from glucose and is important for microorganisms as an energy storage molecule.Development of a cost-effective method for manufacturing biodegradable plastic like P3HB is one of issues for making sustainable future society. Then we will try to extend the applicability of this system for producing other high-value added macromolecules.
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      As a Human Practice project, we are developing a brand new bio-communication tool, named “Biobrick Reviews”.
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      Through this site, everyone can introduce their favorite bio-devices with a short comment for recommendation.
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As a Human Practice project of our team, we are developing a user-friendly bio-device catalog site, named “The Bio-Device Ranking”. Through this site, everyone can introduce their favorite bio-devices with a short comment for recommendation. And everyone including iGEMers can throw a vote to their favorite bio-devices. The goal of this project is not to cover full information of every single bio-device. Our purpose is to contribute to beginner in the field of synthetic biology for learning the best examples of bio-devices through participating this exiting ranking event. Let’s make a nice bio-device catalog together with us!
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      And everyone including iGEMers can throw a vote to their favorite biobricks.
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      The goal of this project is not to cover full information of every single biobrick.
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      Our purpose is to provide a place where every iGEMer or researcher in the field of synthetic biology can find which biobrick is popular through participating this exiting ranking event. Let’s make a nice biobrick catalog together!
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Latest revision as of 17:30, 26 September 2012

Bio-capsule E. coli

Spherical E. coli cluster that can accumulate macromolecules in it

We have designed two modules to make E. coli implementing the function as bio-capsules that accumulate bio-plastic.

First module is to make bio-capsules formed by aggregation of E. coli through cell-cell interaction via adhesion molecule, Ag43, located on the surface of outer membrane. The E. coli capsules can be easily collected by simple filtration without centrifuge steps. Removal of centrifuge steps largely contributes for reduction of time-consuming steps in industrial production of valuable material in E. coli.

Second module is for producing bio-plastic (poly-3-hydroxybutyrate, P3HB). PHB is produced by microorganisms (like Ralstonia eutrophus). The polymer is primarily a product of carbon assimilation from glucose and is important for microorganisms as an energy storage molecule. Development of a cost-effective method for manufacturing biodegradable plastic is one of important issue for making sustainable future society. We have optimized culture conditions for efficient production of E. coli bio-capsules and efficient production of P3HB (first success of bio-plastic production as iGEM team). Then we will try to extend the applicability of this system for producing other high-value added macromolecules in the capsule.

Biobrick Reviews

human practice
As a Human Practice project, we are developing a brand new bio-communication tool, named “Biobrick Reviews”. Through this site, everyone can introduce their favorite bio-devices with a short comment for recommendation. And everyone including iGEMers can throw a vote to their favorite biobricks. The goal of this project is not to cover full information of every single biobrick. Our purpose is to provide a place where every iGEMer or researcher in the field of synthetic biology can find which biobrick is popular through participating this exiting ranking event. Let’s make a nice biobrick catalog together!


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