Team:Bielefeld-Germany/Project/Achievments

:We successfully produced purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL).

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results ECOL]  

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results BPUL]

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results TTHL]

 

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results BHAL]

:We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.   

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]  

:We successfully produced the laccase TVEL5 from ''Trametes versicolor'' in ''P. pastoris'' and showed the activity of the produced enzymes.

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 Results]  

:Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results ECOL]

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results BPUL]

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results TTHL]

:We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. BPUL is able to degrade ethinyl estradiol with addition of ABTS. LC-MS measurements showed degradation products which could not be identified but were further analyzed using MS/MS. The resulting products have been discussed with an organic chemist.

 

:We successfully immobilized ''Trametes versicolor'' laccase (named TVEL0), ''E. coli'' BL21 laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL) and measured their activity.  

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results TVEL0]

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results ECOL]

 

:[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results BPUL]

:We successfully improved a BioBrick by clarifying its true function.  

:[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]

:We successfully created our own database to organize our samples.

 

:We successfully created a model which simulates the oxidation of ABTS.

 

:We conducted a feasibility study of our project with sewage plant experts.

 

:We did an outstanding outreach work.

 

 

[[File:Bielefeld-Germany2012_Goldmedaillie.png‎|30px|frameless|left]]  

:We won a gold medal.

[[File:Bielefeld-Germany2012_Qualified.png‎|30px|frameless|left]]  

:We qualified for the Boston finals