Team:Bielefeld-Germany

From 2012.igem.org

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TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE <br>
TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE <br>
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                             The goal is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds.
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                             Our goal is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds.
                             <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Project/Appoach">
                             <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Project/Appoach">
                             Click here to read more</a> about our project
                             Click here to read more</a> about our project
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To realize our plans we first produce the laccases in the different clades and purify them. After this we immobilized and tested their acitivity.  
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To realize our goal we first produced the laccases in different clades and purified them. After that, we immobilized them and tested their acitivity.  
                             <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary">
                             <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary">
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                             Click here to read more </a> about our methodes...
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                             Click here to read more </a> about our methods...
                           </p>
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                             </div>
   
   
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While working with two different clarification plants we confirmed that our project has the opportunity to solve problems with micro contaminations.     
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Upon working with two different sewage treatment plants, we confirmed that our project has the potential to solve problems of micro contaminations in sewage water.     
                             <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant">
                             <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant">
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                             Click here to read more</a> about the usage in a clarification plants and micro conterminations...</p>                       
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                             Click here to read more</a> about microcontaminents and about the application of our project in clarification plants...</p>                       
                         </div>
                         </div>
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<p align=justify>
<p align=justify>
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The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinylestradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.<br><br>
+
The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinyl estradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.<br><br>
-
The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eucaryotic laccases should be isolated and expressed in ''Escherichia coli'' and ''Pichia pastoris''. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil. </p>
+
The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eukaryotic laccases should be isolated and expressed in <i>Escherichia coli</i> and <i>Pichia pastoris</i>. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil. </p>
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<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]   Creation of a database to organize our samples.
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[[File:Bielefeld-Germany2012_Goldmedaillie.png‎|100px|left]]
 +
 
 +
 
 +
:::Gold medal at the Europe Regional Jamboree
</p>
</p>
 +
<br style="clear: both" />
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]   Production of an active ''E. coli'' Laccase.
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[[File:Bielefeld-Germany2012_Qualified_stemp.png‎‎|100px|left]]
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:::Qualified for the World Championship
</p>
</p>
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<br style="clear: both" />
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Production of an active ''B. pumilus'' Laccase.
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[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Production of four active bacterial laccases
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Production of an active ''T. thermophilus'' Laccase.
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[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Design and assembly of a shuttle vector for site-directed integration in ''P. pastoris''
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Production of an active ''B. halodurans'' Laccase.
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[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Production of an active fungal laccase (TVEL5).
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful characterization of the activity of ''B. pumilus'' Laccase regarding the oxidization of ABTS under different conditions.
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[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Successful characterization of the activity of four bacterial laccases regarding the oxidization of ABTS under different conditions.
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful characterization of the activity of ''E. coli'' Laccase regarding the oxidization of ABTS under different conditions.
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[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Successful immobilization of the ''Trametes versicolor''-laccase (named TVEL0) and measurement of its activity.
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful measurements of enzyme activity of ''T. thermophilus'' Laccase.
+
[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Successful immobilization of four bacterial laccases and measurement of their activity.
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful measurements of enzyme activity of ''B. halodurans'' Laccase.
+
[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Successful creation of a model which simulates the required laccase amount to degraded harmful substances under real sewage plant conditions.
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful immobilization of ''Trametes versicolor''-Laccase (named TVEL0) and measurement of its activity.
+
[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Feasibility study of our project.
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful immobilization of ''E. coli'' BL21-Laccase (CueO named ECOL) and measurement of its activity.
+
[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]   
 +
:Creation of a database to organize our samples.
</p>
</p>
<p>
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]   Successful immobilization of ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL) and measurement of its activity.
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[[File:Bielefeld-Germany2012_Check.jpg|20px|left]]
-
</p>
+
:Improvement of a BioBrick by clarifying its true function.
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<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Improved a BioBrick by clarifying its true function.
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-
</p>
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-
<p>
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[[File:Bielefeld-Germany2012_Check.jpg|15px|left]]  Successful creation of a modell which simulates the oxidation of ABTS.
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</p>
</p>
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  <td> <a href="http://www.youtube.com/user/iGEMBielefeld"><img src="https://static.igem.org/mediawiki/2012/9/9f/Cattube.jpg" /> </a> </td>
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  <td> <a href="http://www.facebook.com/pages/iGEM_Bielefeld/244094752286371"><img src="https://static.igem.org/mediawiki/2012/2/20/Gesichtsbuch.jpg" /> </a> </td>
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  <td> <a href="http://twitter.com/iGEM_Bielefeld"><img src="https://static.igem.org/mediawiki/2012/d/db/Zwitscher.jpg" /> </a> </td>
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  <td> <a href="http://www.flickr.com/photos/igem-bielefeld"><img src="https://static.igem.org/mediawiki/2012/b/ba/Leckr.jpeg" width="76" /> </a> </td>
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| [[File:Promega logo 180x109.gif|120px|link=http://www.promega.com/]] ||
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| [[Image:Logo merck.jpg|100px|link=http://www.merckgroup.com/en/index.html]] ||
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| [[File:BioCircle.JPG|100px|link=http://www.bio-circle.de/home.html]]  ||
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| [[File:Bielefeld2012 Evonik.jpg|140px|link=http://www.evonik.de]] ||
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| [[File:Bielefeld2012_Baxter.png|100px|link=http://www.baxter.de]]  
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|[[File:Bielefeld2012_Baxter.png|180px|link=http://www.baxter.de]] || [[File:Logo knauer.jpg|200px|link=http://www.knauer.net/]] || [[File:Logo iit.jpg|150px|link=http://www.iit-biotech.de]] ||
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[[File:logo biometra.jpg|200px|link=http://www.biometra.de ]]
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| [[File:Logo iit.jpg|100px|link=http://www.iit-biotech.de]] ||
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| [[File:logo biometra.jpg|150px|link=http://www.biometra.de ]] ||
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| [[File:Bielefeld2012_BIEKUBA.jpg|100px|link=http://www.iit-unibi.de/]] ||
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| [[File:Bielefeld2012_Logo_ERASynbio.jpg|100px|link=http://www.erasynbio.net/]]
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|-
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| [[File:Logo bio-nrw.png|100px|link=http://www.bio.nrw.de/en]] ||
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| [[File:Bielefeld-Germany-CeBiTec.jpg|100px|link=http://www.cebitec.uni-bielefeld.de/]] ||  
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| [[File: IgemBielefeld_2011_Logo_uni.jpg|100px|link=http://www.uni-bielefeld.de]] ||
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| [[File:IgemBielefeld_Logo_biofak.jpg|100px|link=http://www.uni-bielefeld.de/biologie]] ||
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| [[File:Bielefeld-Uniersity-techfak.gif|100px|link=http://www.techfak.uni-bielefeld.de/web/ ]]
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|[[File:Logo bio-nrw.png|100px|link=http://www.bio.nrw.de/en]] || [[File:Bielefeld-Germany-CeBiTec.jpg|130px|link=http://www.cebitec.uni-bielefeld.de/]] || [[File: IgemBielefeld_2011_Logo_uni.jpg|130px|link=http://www.uni-bielefeld.de]] || [[File:IgemBielefeld_Logo_biofak.jpg|130px|link=http://www.uni-bielefeld.de/biologie]] || [[File:Bielefeld-Uniersity-techfak.gif|130px|link=http://www.techfak.uni-bielefeld.de/web/ ]]
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<div class="about">
<div class="about">
<p>About our Wiki</p>
<p>About our Wiki</p>
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<p>Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach. </p>
+
<p>Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In this Wiki we document our approach and our achievements. </p>
</div>
</div>
         <div class="contact">
         <div class="contact">
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                 <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Project/Description">Project</a></p>
                 <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Project/Description">Project</a></p>
                 <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary">Results</p>
                 <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary">Results</p>
 +
                <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Amsterdam/Results">Since Regionals</p>
                 <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview">Human Practice</a></p>
                 <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview">Human Practice</a></p>
                 <p><a href='https://2012.igem.org/Team:Bielefeld-Germany/Safety'>Safety</a>
                 <p><a href='https://2012.igem.org/Team:Bielefeld-Germany/Safety'>Safety</a>
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                 <p><a href='https://2012.igem.org/Team:Bielefeld-Germany/Partner'>Partner</a></p>
                 <p><a href='https://2012.igem.org/Team:Bielefeld-Germany/Partner'>Partner</a></p>
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Latest revision as of 17:59, 14 June 2013

  • Our Plan
    TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE
    Our goal is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds.

    Click here to read more about our project

  • The Realization
    To realize our goal we first produced the laccases in different clades and purified them. After that, we immobilized them and tested their acitivity.

    Click here to read more about our methods...

  • The Potential
    Upon working with two different sewage treatment plants, we confirmed that our project has the potential to solve problems of micro contaminations in sewage water.

    Click here to read more about microcontaminents and about the application of our project in clarification plants...

  • Laccases - Our Teammate
    Laccases have the ability to oxidize phenolic and nonphenolic lignin related compounds as well as highly recalcitrant environmental pollutants. This makes them very useful for applications concerning several biotechnological processes.

    Click here to read more about laccases...

Welcome

The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinyl estradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.

The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eukaryotic laccases should be isolated and expressed in Escherichia coli and Pichia pastoris. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil.

A Case for Laccase!
iGEM Team Bielefeld 2012
Achievements

Bielefeld-Germany2012 Goldmedaillie.png


Gold medal at the Europe Regional Jamboree


Bielefeld-Germany2012 Qualified stemp.png


Qualified for the World Championship


Bielefeld-Germany2012 Check.jpg
Production of four active bacterial laccases

Bielefeld-Germany2012 Check.jpg
Design and assembly of a shuttle vector for site-directed integration in P. pastoris

Bielefeld-Germany2012 Check.jpg
Production of an active fungal laccase (TVEL5).

Bielefeld-Germany2012 Check.jpg
Successful characterization of the activity of four bacterial laccases regarding the oxidization of ABTS under different conditions.

Bielefeld-Germany2012 Check.jpg
Successful immobilization of the Trametes versicolor-laccase (named TVEL0) and measurement of its activity.

Bielefeld-Germany2012 Check.jpg
Successful immobilization of four bacterial laccases and measurement of their activity.

Bielefeld-Germany2012 Check.jpg
Successful creation of a model which simulates the required laccase amount to degraded harmful substances under real sewage plant conditions.

Bielefeld-Germany2012 Check.jpg
Feasibility study of our project.

Bielefeld-Germany2012 Check.jpg
Creation of a database to organize our samples.

Bielefeld-Germany2012 Check.jpg
Improvement of a BioBrick by clarifying its true function.

Our Partners

120px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png
Logo knauer.jpg Logo iit.jpg Logo biometra.jpg Bielefeld2012 BIEKUBA.jpg Bielefeld2012 Logo ERASynbio.jpg
Logo bio-nrw.png Bielefeld-Germany-CeBiTec.jpg IgemBielefeld 2011 Logo uni.jpg IgemBielefeld Logo biofak.jpg 100px


About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In this Wiki we document our approach and our achievements.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

Navigate our Wiki

Start

Team

Project

Results

Since Regionals

Human Practice

Safety

Judging

Partner

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