Team:HokkaidoU Japan/Notebook/aggregation Week 13

From 2012.igem.org

(Difference between revisions)
(26th characterization of aggregation)
 
(3 intermediate revisions not shown)
Line 4: Line 4:
<!-- DO NOT EDIT OVER THIS LINE @iTakeshi -->
<!-- DO NOT EDIT OVER THIS LINE @iTakeshi -->
<div class="hokkaidou-notebook-daily">
<div class="hokkaidou-notebook-daily">
-
==September 26th==
+
===September 26th===
-
<div>
+
<div class="hokkaidou-section">
-
==Characterization of Aggregation==
+
====Characterization of Aggregation====
-
Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT construct.
+
Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT-pSB1C3 construct.
#Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap.
#Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap.
#Suspended colonies into the medium.
#Suspended colonies into the medium.
Line 13: Line 13:
#Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager.
#Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager.
-
Result
+
''Result''<br />
-
</div>
+
We couldn't observe eCFP expression, but OD600 of suspension has difference between Arabinose added culture and negative control.
 +
Data(OD600)
 +
{|
 +
|2(hour)
 +
|4
 +
|6
 +
|8
 +
|10
 +
|12
 +
|-
 +
|Ara+
 +
|-
 +
|0.0119457
 +
|0.1101659
 +
|0.013273 
 +
|0.1486576
 +
|0.2707692
 +
|0.3663348
 +
|-
 +
|Ara-
 +
|-
 +
|0.0119457
 +
|0.1499849
 +
|0.2813876
 +
|1.0419305
 +
|1.1932427
 +
|1.221116
 +
|}
 +
 
 +
<br style="line-height: 0; clear: both;" />
 +
</div></div>
<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi -->
<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi -->
</div>
</div>
<br style="line-height: 0; clear: both;" />
<br style="line-height: 0; clear: both;" />
{{Team:HokkaidoU_Japan/footer}}
{{Team:HokkaidoU_Japan/footer}}

Latest revision as of 03:26, 27 September 2012

September 26th

Characterization of Aggregation

Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT-pSB1C3 construct.

  1. Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap.
  2. Suspended colonies into the medium.
  3. Incubated at 37C for 12 hrs at 180 rpm. For this incubation, pipetted 150 ul of this cultivating medium to 96-well microtiter plate every 2 hrs.
  4. Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager.

Result
We couldn't observe eCFP expression, but OD600 of suspension has difference between Arabinose added culture and negative control. Data(OD600)

2(hour) 4 6 8 10 12
Ara+
0.0119457 0.1101659 0.013273 0.1486576 0.2707692 0.3663348
Ara-
0.0119457 0.1499849 0.2813876 1.0419305 1.1932427 1.221116



Retrieved from "http://2012.igem.org/Team:HokkaidoU_Japan/Notebook/aggregation_Week_13"