Team:Goettingen/week10-1

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(Difference between revisions)
 
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The cells are dropped onto M9-medium, that are prepared with a 0.5% trypton solution soaked whatman paper. The used strains are DH10B, DH5alpha, XL1, and BL21, each in four variations (empty vector, 18C-, 18O-, 18M-vector)</li>
The cells are dropped onto M9-medium, that are prepared with a 0.5% trypton solution soaked whatman paper. The used strains are DH10B, DH5alpha, XL1, and BL21, each in four variations (empty vector, 18C-, 18O-, 18M-vector)</li>
<li>Observations & Results:<br>
<li>Observations & Results:<br>
-
On the next day, there was no swimming visible at all. After two more (three in total) days, there was strong swimming for the BL21 strains detectable. ∆tar and DH10B strains showed minimal, DH5alpha and XL1 no swimming
+
On the next day, there was no swimming visible at all. After two more (three in total) days, there was strong swimming for the BL21 strains detectable. <i>∆tar</i> and DH10B strains showed minimal, DH5alpha and XL1 no swimming
</ul>
</ul>
<b>V07_03_2: Test of a variety of attractant solutions for the whatman paper</b><br>
<b>V07_03_2: Test of a variety of attractant solutions for the whatman paper</b><br>
<ul><li>Experiment:<br>
<ul><li>Experiment:<br>
-
The swimming assay was performed with ∆tar strains (with empty vector, 18C, and 18M) on M9 agar. For the whatman paper a variety of solutions was tested: Asp+Met, Asp+Leu, Asp+Leu+Met, Trypton, H20. Each solution was used in two different variations, w/ and w/o fluorescin to detect its diffusion over the agar plate.
+
The swimming assay was performed with <i>∆tar</i> strains (with empty vector, 18C, and 18M) on M9 agar. For the whatman paper a variety of solutions was tested: Asp+Met, Asp+Leu, Asp+Leu+Met, Trypton, H<sub>2</sub>0. Each solution was used in two different variations, w/ and w/o fluorescin to detect its diffusion over the agar plate.
<li>Observations & Results:<br>
<li>Observations & Results:<br>
On the next day, none of the plates showed any swimming. Also, the fluorescin concentration must have been to low, because no fluorescence was detectable under UV light.
On the next day, none of the plates showed any swimming. Also, the fluorescin concentration must have been to low, because no fluorescence was detectable under UV light.
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<ul>
<ul>
<li>Experiment: <br>
<li>Experiment: <br>
-
wild-type strains without any transformed plasmids were dropped onto M9 agar plates, to identify those that are sensitive for the used chemoattractants (0.5% trypton solution and 50 mM aspartate solution). The chemoattractant was incoroporated into the whatman paper, which was put in the middle of the agar plate. Furthermore, the influence of chemotaxis-washing-buffer was tested. The approach from above was done twice, once w/ and once w/o a washing step.</li>
+
Wild-type strains without any transformed plasmids were dropped onto M9 agar plates, to identify those that are sensitive for the used chemoattractants (0.5% trypton solution and 50 mM aspartate solution). The chemoattractant was incoroporated into the Whatman paper, which was put in the middle of the agar plate. Furthermore, the influence of chemotaxis-washing-buffer was tested. The approach from above was done twice, once w/ and once w/o a washing step.</li>
</ul>
</ul>
<br></td></tr>
<br></td></tr>

Latest revision as of 18:33, 26 September 2012

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#1 Selection / Swimming - 10th Week

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V07_03


V07_03_1: Newly transformed cells are tested via the trypton-whatman-assay
  • Experiment:
    The cells are dropped onto M9-medium, that are prepared with a 0.5% trypton solution soaked whatman paper. The used strains are DH10B, DH5alpha, XL1, and BL21, each in four variations (empty vector, 18C-, 18O-, 18M-vector)
  • Observations & Results:
    On the next day, there was no swimming visible at all. After two more (three in total) days, there was strong swimming for the BL21 strains detectable. ∆tar and DH10B strains showed minimal, DH5alpha and XL1 no swimming
V07_03_2: Test of a variety of attractant solutions for the whatman paper
  • Experiment:
    The swimming assay was performed with ∆tar strains (with empty vector, 18C, and 18M) on M9 agar. For the whatman paper a variety of solutions was tested: Asp+Met, Asp+Leu, Asp+Leu+Met, Trypton, H20. Each solution was used in two different variations, w/ and w/o fluorescin to detect its diffusion over the agar plate.
  • Observations & Results:
    On the next day, none of the plates showed any swimming. Also, the fluorescin concentration must have been to low, because no fluorescence was detectable under UV light.

V07_05


Comparison of several attractans for chemotaxis assays
  • Experiment:
    Wild-type strains without any transformed plasmids were dropped onto M9 agar plates, to identify those that are sensitive for the used chemoattractants (0.5% trypton solution and 50 mM aspartate solution). The chemoattractant was incoroporated into the Whatman paper, which was put in the middle of the agar plate. Furthermore, the influence of chemotaxis-washing-buffer was tested. The approach from above was done twice, once w/ and once w/o a washing step.


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