Team:Goettingen/week8-1

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Latest revision as of 18:29, 26 September 2012


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V-Gradiant agar plates (Variation of V06_14)

Experiment:
This time, the plates were poured in three steps. This should result in a more directed, triangular gradient. Furthermore, the bacteria were dropped on random spots, to identify the best dropping position.

Swimmimg assay with M9-agar and Whatman-paper

Experiment:
"M9 agar plates" (4 mL 100% glycerol, 80 mL 5x M9 salt solution, 400 µL CaCl2*2H20 [20 mg/mL], 400 µL MgSO4 [0.12 g/mL], 0.3% agar, add 400 mL H2O) were produced and used for swimming assays, as explained in methods. The Whatman papers was incorporated with 100 µL of a 50 mM aspartate solution.
Observations & Results:
After about 15h were no swimming visible. The only exception was the BL21 strain, which showed a little swimming.

@@ Line 30: / Line 30: @@
 <b>V-Gradiant agar plates (Variation of V06_14)</b><br>
 <ul>
-<li>Experiment: <br>This time, the plates were poured in three steps. This should result in a more directed, triangular gradient. Furthermore, the bacteria were dropped on radom spots, to identify the best dropping position.</li>
+<li>Experiment: <br>This time, the plates were poured in three steps. This should result in a more directed, triangular gradient. Furthermore, the bacteria were dropped on random spots, to identify the best dropping position.</li>
 </ul>
 <br></td></tr>
@@ Line 43: / Line 43: @@
 <b>Swimmimg assay with M9-agar and Whatman-paper</b><br>
 <ul>
-<li>Experiment: <br> "M9 agar plates" (4 mL 100% glycerol, 80 mL 5x M9 salt solution, 400 µL CaCl2*2H20 [20 mg/mL], 400 µL MgSO4 [0.12 g/mL], 0.3% agar, add 400 mL H2O) were produced and used for swimming assays, as explained in methods. The whatman papers was incorporated with 100 µL of a 50 mM aspartate solution.</li>
+<li>Experiment: <br> "M9 agar plates" (4 mL 100% glycerol, 80 mL 5x M9 salt solution, 400 µL CaCl2*2H20 [20 mg/mL], 400 µL MgSO4 [0.12 g/mL], 0.3% agar, add 400 mL H2O) were produced and used for swimming assays, as explained in methods. The Whatman papers was incorporated with 100 µL of a 50 mM aspartate solution.</li>
 <li> Observations & Results:<br>
 After about 15h were no swimming visible. The only exception was the BL21 strain, which showed a little swimming.