Team:BostonU/Protocols

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<div id="bu-wellesley_wiki_content">
<div id="bu-wellesley_wiki_content">
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<p  style="text-align:center;"><a href="https://2012.igem.org/Team:BostonU"><img src="http://i.imgur.com/adRd9.png" width="800px"></a></p>
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<p  style="text-align:center;"><a href="https://2012.igem.org/Team:BostonU"><img src="https://static.igem.org/mediawiki/2012/2/2d/NotebookBU.png" width="800px"></a></p>
<ul id="nav">  
<ul id="nav">  
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<li><a href="#">Team</a>  
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<li><a href="#">Our Team</a>  
<ul>
<ul>
<li><a href="https://2012.igem.org/Team:BostonU/Team">Team</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Team">Team</a></li>
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<ul>  
<ul>  
<li><a href="https://2012.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>  
<li><a href="https://2012.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Characterization">Introduction to Characterization</a></li>  
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                        <li><a href="https://2012.igem.org/Team:BostonU/MoClo2">Introduction to MoClo</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Characterization">Introduction to Characterization</a></li>
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                        <li><a href="https://2012.igem.org/Team:BostonU/DataSheet">Introduction to Data Sheets</a></li> 
<li><a href="https://2012.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>  
<li><a href="https://2012.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Results ">Results Summary</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/Results ">Results Summary</a></li>
</ul>  
</ul>  
</li>  
</li>  
<li><a href="#">Achievements</a>  
<li><a href="#">Achievements</a>  
<ul>  
<ul>  
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<li><a href="https://2012.igem.org/Team:BostonU/Data">Data</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Data">Data Collected</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Parts">Parts</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>  
<li><a href="https://2012.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>  
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<ul>
<ul>
<li><a href="https://2012.igem.org/Team:BostonU/Protocols">Protocols</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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<li><a href="https://2012.igem.org/Team:BostonU/Clotho">Clotho and Eugene</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Notebook">Weekly Notebook</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Notebook">Weekly Notebook</a></li>
</ul>
</ul>
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<li><a href="#">Considerations</a>  
<li><a href="#">Considerations</a>  
<ul>
<ul>
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<li><a href="https://2012.igem.org/Team:BostonU/Advancement">Synthetic Biology Advancement</a></li>  
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<li><a href="https://2012.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>  
<li><a href="https://2012.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>  
<li><a href="https://2012.igem.org/Team:BostonU/Safety">Safety</a></li>
<li><a href="https://2012.igem.org/Team:BostonU/Safety">Safety</a></li>
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</ul>
</ul>
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<li><a href="https://2012.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
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<li><a href="#">Acknowledgements</a>
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<ul>
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                    <li><a href="https://2012.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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                    <li><a href="https://2012.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
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                </ul>
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</li>
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<br>
<br>
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<h6>Protocols</h6>
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<h4>Protocols</h4>
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<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p>
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We hope they help anyone interested in our experiments to successfully repeat them.
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<br>
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<br>
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<br>
<br>
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<h3>Coming soon!</h3>
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<li><a href="https://static.igem.org/mediawiki/2012/0/0b/Phusion_PCRblank.xls">Phusion PCR </a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/c/cb/Transformation_Gold_Bioline_cells.xls">Transformation with Gold Bioline Cells</a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/0/05/Restriction_Digest.xls">Restriction Digest </a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/8/8e/Qiagen_QIAquick_Gel_extraction.xls">Gel Extraction </a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/5/58/Qiagen_QIAprep_Miniprep.xls">Miniprep </a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/7/7e/NanoDrop_DNA_Quantification.xls">DNA Quantification </a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/b/bd/Media_Preparation_LB_broth_agar_Gels.xls">Media Prep </a></p>
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<li><a href="https://static.igem.org/mediawiki/2012/1/1f/Ligation_Regular_and_MoClo.xls">Ligation </a></p>
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</html>

Latest revision as of 23:40, 19 May 2013

BostonU iGEM Team: Welcome


Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.


  • Phusion PCR

  • Transformation with Gold Bioline Cells

  • Restriction Digest

  • Gel Extraction

  • Miniprep

  • DNA Quantification

  • Media Prep

  • Ligation

    Retrieved from "http://2012.igem.org/Team:BostonU/Protocols"