Team:Goettingen/week21-3

From 2012.igem.org

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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
 
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<b>Synthesis of cDNA and qrtPCR</b><br>
<b>Synthesis of cDNA and qrtPCR</b><br>
<ul>
<ul>
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<li>Experiment: <br>cDNA synthesis was performed using Reverse Transcription Kit (Qiagen).<br>
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<li>Experiment: <br>cDNA synthesis of RNA from <a href="https://2012.igem.org/Team:Goettingen/week20-3">V09_16</a>  was performed using Reverse Transcription Kit (Qiagen).<br>
qrtPCR with promoter constructs was performed, for a detailed protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Experimental_design">click here</a>.<br>
qrtPCR with promoter constructs was performed, for a detailed protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Experimental_design">click here</a>.<br>
</li>
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<li>Observations & Results:<br>
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CP-values of triplets showed values in a range which were useful for <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Analysis_via_the_2.E2.80.93.CE.94.CE.94CT_.28Livak.29_Method">calculation</a> of expression rates. </li>
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CP-values of triplets showed values in a range which were useful for <a href="https://2012.igem.org/Team:Goettingen/Project/Methods#-.3E_Analysis_via_the_2.E2.80.93.CE.94.CE.94CT_.28Livak.29_Method">analysis via the 2<sup>–ΔΔCT</sup> (Livak) method</a> of expression rates. </li>
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<h2><b>V09_17-23</b></h2><br>
 +
<b>Generation of BioBrick pSB1C3_TAR_QC without promoter</b><br>
 +
<ul>
 +
<li>Experiment: <br>TAR_QC was cloned into pSB1C3 without the promoter constructs.<br>
 +
</li>
 +
<li>Observations & Results:<br>This part is now ready to be send in as BioBrick (see our <a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts">submitted parts</a>).
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<br>
<br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
 
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{{GoettingenFooter}}

Latest revision as of 20:45, 26 September 2012

Deutsch  / English 

#3 Chemoreceptor Library - 21th Week

Back to overview

V09_17


Synthesis of cDNA and qrtPCR
  • Experiment:
    cDNA synthesis of RNA from V09_16 was performed using Reverse Transcription Kit (Qiagen).
    qrtPCR with promoter constructs was performed, for a detailed protocol click here.
  • Observations & Results:
    CP-values of triplets showed values in a range which were useful for analysis via the 2–ΔΔCT (Livak) method of expression rates.


V09_17-23


Generation of BioBrick pSB1C3_TAR_QC without promoter
  • Experiment:
    TAR_QC was cloned into pSB1C3 without the promoter constructs.
  • Observations & Results:
    This part is now ready to be send in as BioBrick (see our submitted parts).


Back to overview
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