Team:Goettingen/week6-1

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<h2><b>VX_Y </b></h2><br>
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<h2><b>V06_04 </b></h2><br>
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<b>V06_04_1: preparation of swimming cultures and LB-swimming plates to perform swimming assay</b><br>
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<li>Experiment: <br>hier text reinschreiben</li>
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<li>Experiment: <br>LB-Agar plates, composed of concentrations of 0.5% or 0.3% in addition to the standard LB-components were produced. Afterwards, 1 µL of DH10B-strains with P1-18C and P1-18M constructs were dropped onto plates. </li>
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<h2><b>V06_05 </b></h2><br>
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<b>V06_05_1: Different promotor strengths were tested on different agar compositions.</b><br>
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<li>Experiment: <br>1 µL, 2 µL, and 5 µL of transformed strains (plasmids used: P1-18C-K3, P1-20E-K3, P1-18M-K2, P1-18M-K1, P1-18C-K2, P1-20E-K2) were tested on two different swimming agars, each. The agar was composed of: 1) 0.3% agar, 0.5% glucose, 1% yeast extract; 2) 0.3% agar, 0.5% glucose, 0,33% beef extract.</li>
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<li>Observations & Results: <br> The best swimming/growing conditions that could be identified were agar 1), no swimming at agar 2) and 5 µL of dropped baceria solution. The plates with 1 µL and 2 µL did not show any swimming/growth.</li>
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Latest revision as of 17:01, 25 September 2012

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#1 Selection / Swimming - 6th Week

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V06_04


V06_04_1: preparation of swimming cultures and LB-swimming plates to perform swimming assay
  • Experiment:
    LB-Agar plates, composed of concentrations of 0.5% or 0.3% in addition to the standard LB-components were produced. Afterwards, 1 µL of DH10B-strains with P1-18C and P1-18M constructs were dropped onto plates.


V06_05


V06_05_1: Different promotor strengths were tested on different agar compositions.
  • Experiment:
    1 µL, 2 µL, and 5 µL of transformed strains (plasmids used: P1-18C-K3, P1-20E-K3, P1-18M-K2, P1-18M-K1, P1-18C-K2, P1-20E-K2) were tested on two different swimming agars, each. The agar was composed of: 1) 0.3% agar, 0.5% glucose, 1% yeast extract; 2) 0.3% agar, 0.5% glucose, 0,33% beef extract.
  • Observations & Results:
    The best swimming/growing conditions that could be identified were agar 1), no swimming at agar 2) and 5 µL of dropped baceria solution. The plates with 1 µL and 2 µL did not show any swimming/growth.


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