Team:Bielefeld-Germany/Labjournal/week10

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==Week 10 (07/02 - 07/08/12)==
 
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===Monday July 2nd===
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'''Team Fungal Laccases''': Sending a request for  plasmids containing cDNA sequences of five different laccases from ''Trametes versicolor'' and ''Pycnoporus cinnabarinus'' from the Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis at Ernst-Moritz-Arndt-University in Greifswald.
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* '''Team Student Academy:''' During the whole week the two presentations for school academy were prepared and the barbecue for the pupils was organized. In the lab the last preparations were made.
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===Tuesday July 3rd===
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* '''Team Site Directed Mutagenesis:''' Decided how to insert silent mutations to get rid of the restriction-sites with an eye on the codon-usage of host- and scource-oragnism, using [http://www.kazusa.or.jp/codon/ the Kazusa “Codon Usage Database”]
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**ecol’s illegal ''Ngo''MIV will be deleted by changing ggG to ggA (Glycin) at 2307
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**tthl’s illegal ''Pst''I will be deleted by changing caG to caA (Glutamine) at 2796
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**bpul’s illegal ''Xba''I will be deleted by changing  ctA to ctT (Leucin) at 2883
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**bpul’s mutation will be deleted by changing Gag (Glutamat) to Aag (Lysin) at 2317
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**xccl’s first illegal ''Pst''I will be deleted by changing ctG to ctC (Valine) at 2247
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**xccl’s second illegal ''Pst''I will be deleted by changing ctG to ctC (Valine) at 3633
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===Wednesday July 4th===
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* '''Team Student Academy:''' Final meeting with all instructors of the student academy to talk about all details.
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li>
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* '''Team Bacterial Laccases:'''
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:*After the failed attempts to isolate the laccase genes from ''Streptomyces lavendulae'' and ''Streptomyces griseus'' we assumed that maybe the sequences from this laccases and the laccase sequences we designed the primers for were to different to get a product [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th (look here for more details)]. So we used the sequences of the laccase genes we tried to isolate from the ''Streptomycetes'' strains and blasted them against an intern database from our university. The results showed that we could use genomic DNA from ''Streptomyces sp. tuebingen'', ''S.roseochromogenes'' and ''Streptomyces sp. goettingen'', from which we can get already isolated genomic DNA. Therefore we designed primers for the isolation of the predicted laccase genes from these three strains.
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===Thursday July 5th===
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* '''Team shuttle vector:''' Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the primer pair pSB1C3-5aox1-f and pSB1C3-5aox1-r. The fragment MF-alpha1 was amplified with the primer pair 5aox1-mfalpha1-f and 5aox1-mfalpha1-r. The fragment 3'AOX1 was amplified with the primer pair his4-3aox1-f and his4-3aox1-r. The fragment pSB1C3 was amplified with the primer pair 3aox1-pSB1C3-f and 3aox1-pSB1C3-r.
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==Week 10 (07/02 - 07/08/12)==
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===Friday July 6th===
 
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===Saturday July 7th===
 
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===Sunday July 8th===
 
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Sunday
 
{{Team:Bielefeld/Sponsoren}}
{{Team:Bielefeld/Sponsoren}}

Latest revision as of 21:19, 25 September 2012


Week 10 (07/02 - 07/08/12)

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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