Team:Goettingen/week3-3

From 2012.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
-
{{GoettingenHeader|deu=Team:Goettingen/Notebook|eng=Team:Goettingen/Notebook}}
+
{{GoettingenHeader|deu=Team:Goettingen/week3-3|eng=Team:Goettingen/week3-3}}
<html>
<html>
<table>
<table>
Line 40: Line 40:
<b>Isolation of pUC18 plasmid DNA</b><br>
<b>Isolation of pUC18 plasmid DNA</b><br>
<ul>
<ul>
-
<li>Experiment: <br>Isolation of pUC18 from DH10B using PeqGOLD MiniPrep Kit (Peqlab).
+
<li>Experiment: <br>Isolation of pUC18 from DH10B using peqGOLD Miniprep Kit (Peqlab).
</li>
</li>
</ul>
</ul>

Latest revision as of 11:22, 22 September 2012

Deutsch  / English 

#3 Chemoreceptor Library - 3rd Week

Back to overview

V05_15


Preparing Media
  • Experiment:
    Preparation of LB medium and LB-Amp plates (recipe see here). Preparing streak plates of pUC18 DH10B on LB-Amp, incubation at 37 °C.


V05_16


Isolation of pUC18 plasmid DNA
  • Experiment:
    Isolation of pUC18 from DH10B using peqGOLD Miniprep Kit (Peqlab).


V05_18


Insertion of tar into pUC18
  • Experiment:
    Double digest of tar and pUC18 using EcoRI, PstI and Orange Buffer (ThermoScientific). Preparation of chemical competent DH10B cells for transformation.


Back to overview

↑ Back to top!

Retrieved from "http://2012.igem.org/Team:Goettingen/week3-3"