Team:Goettingen/week2-2

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                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/Team#responsibitlities"><span><span><div style="text-indent:20px;">&#8901; Responsibilities</div></span></span></a></li> -->
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Focus_Groups"><span><span>Focus Groups</span></span></a></li>
 
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group1"><span><span><div style="text-indent:20px;">&#8901; #1 Selection / Swimming</div></span></span></a></li>  -->
 
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                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group2"><span><span><div style="text-indent:20px;">&#8901; #2 Speed Improvement</div></span></span></a></li>  -->
 
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                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group3"><span><span><div style="text-indent:20px;">&#8901; #3 Chemoreceptor</div></span></span></a></li>    -->
 
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                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#library"><span><span><div style="text-indent:20px;">&#8901; Library</div></span></span></a></li>            -->
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Seminars"><span><span>Seminars</span></span></a></li>
 
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#announce"><span><span><div style="text-indent:20px;">&#8901; Announcements</div></span></span></a></li>    -->
 
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                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#presentations"><span><span><div style="text-indent:20px;">&#8901; Past Presentations</div></span></span></a></li> -->
 
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Work_Impressions"><span><span>Work Impressions</span></span></a></li>
 
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                        <a href="https://2012.igem.org/Team:Goettingen/Project" style="color: white;">Project<!--[if gt IE 6]><!--></a><!--<![endif]-->
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Project"><span><span>Our Project</span></span></a></li>
 
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                        <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Chemotaxis"><span><span><div style="text-indent:20px;">&#8901; Chemotaxis</div></span></span></a></li>  -->
 
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                        <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Poster"><span><span><div style="text-indent:20px;">&#8901; Poster</div></span></span></a></li>    -->
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Materials"><span><span>Materials</span></span></a>
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Methods"><span><span>Methods</span></span></a>
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Computational_Data"><span><span>Computational Data</span></span></a>
 
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                        <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">&#8901; Maps</div></span></span></a></li>
 
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                        </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">&#8901; Sequences</div></span></span></a></li> -->
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Bioinformatical_Tool"><span><span>Bioinformatical Tools</span></span></a>
 
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                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Lab_Work_Flow"><span><span>Lab Work Flow</span></span></a>
 
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                        <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">&#8901; Golden Lab Rules</div></span></span></a></li>
 
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                        </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">&#8901; Daily Check List</div></span></span></a></li> -->
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook"><span><span>Overview</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary"><span><span>Summary</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Discussion"><span><span>Discussion</span></span></a></li>
 
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                        <a href="https://2012.igem.org/Team:Goettingen/iGEM" style="color: white;">iGEM<!--[if gt IE 6]><!--></a><!--<![endif]-->
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM"><span><span>What is iGEM?</span></span></a></li>
 
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                                <!--<li><a href="https://2012.igem.org/Team:Goettingen/BioBrick_System"><span><span>iGEM BioBrick System</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Part_Registry"><span><span>iGEM Part Registry</span></span></a></li>  -->
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts_Submitted"><span><span>Parts Submitted</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Attributions"><span><span>Attributions</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Saftey"><span><span>Saftey</span></span></a></li>>
 
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                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 
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                                <ul class="DropDownMenu" id="MB1-DDM5">
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen"><span><span>City</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/University"><span><span>Georg-August-University</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/MPI"><span><span>Max-Planck-Institute</span></span></a></li>
 
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                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/S1-Demo_Lab"><span><span>S1-Demo Lab</span></span></a></li>
 
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                        <a href="https://2012.igem.org/Team:Goettingen/Human_Practice" style="color: white;">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
 
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                                <ul class="DropDownMenu" id="MB1-DDM5">
 
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                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice"><span><span>Why Human Practice?</span></span></a></li>
 
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Public_and_Media"><span><span>Public and Media</span></span></a></li>
 
-
                              <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">&#8901; Newspaper</div></span></span></a></li>
 
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                              </li><li><a href="https://2012.igem.org/Team:Goettingen/Conference"><span><span><div style="text-indent:20px;">&#8901; Conference</div></span></span></a></li>
 
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                              </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">&#8901; Synthetic Biology Day</div></span></span></a></li> -->
 
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Survey"><span><span>Survey</span></span></a></li>
 
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion"><span><span>Panel Discussion</span></span></a></li>-->
 
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
 
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                              </ul>
 
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                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
 
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-
                        <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
 
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
 
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                                <ul class="DropDownMenu" id="MB1-DDM2-SM3">
 
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                              <li><a href="https://2012.igem.org/Team:Goettingen/Sponsoring"><span><span>Interested in...?</span></span></a></li>
 
-
                                <!-- <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Sponsors"><span><span>Sponsors</span></span></a></li>
 
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Supporters"><span><span>Supporters</span></span></a></li>    -->
 
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                </li>
 
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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
 
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<b>Amplification of <i>flhDC</i> gene</b><br>
<b>Amplification of <i>flhDC</i> gene</b><br>
<ul>
<ul>
-
<li>Experiment: <br> Using the isolated genomic DNA of DH10B as template, the gene <i>flhDC</i> was amplified by <i>pfu</i> polymerase according to the following <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.</li>
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<li>Experiment: <br> Using the isolated genomic DNA of <i>E. coli</i> (DH10B) as template, the gene <i>flhDC</i> was amplified by <i>pfu</i> polymerase according to the following <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.</li>
<br> </li>
<br> </li>
</ul>
</ul>
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<ul>
<ul>
<li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
<li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
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<a href="#top">&uarr; Return to top</a>
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<br>
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<br>
<br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<br>
<br>
</td>
</td>
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<table bordercolor="black" border="1 px" width="600 px"><tr><td>
 
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<b>Important pages</b>:<br>
 
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<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
 
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<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
 
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</td></tr>
 
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</table>
 
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{{GoettingenFooter}}

Latest revision as of 17:17, 22 September 2012

Deutsch  / English 

#2 Speed Improvement - 2nd week

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V05_08


Amplification of flhDC gene
  • Experiment:
    Using the isolated genomic DNA of E. coli (DH10B) as template, the gene flhDC was amplified by pfu polymerase according to the following protocol.



V05_10


Separation of flhDC
  • Experiment:
    The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
  • Observations & Results:
    The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.


V05_11


Purificaton of flhDC
  • Experiment:
    The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.


Back to overview

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