Team:Goettingen/week21-2

From 2012.igem.org

(Difference between revisions)
 
(22 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
+
{{GoettingenHeader|deu=Team:Goettingen/week1|eng=Team:Goettingen/week1}}
-
                <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
+
<html>
-
                                <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
+
<table>  
-
                <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
+
-
                <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
+
-
                <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&amp;action=creativecommons" rel="meta">
+
-
<link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=rss">
+
-
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=atom">
+
-
 
+
-
 
+
-
<body class="mediawiki ns-0 ltr page-Team_Goettingen">
+
-
 
+
-
 
+
-
 
+
-
 
+
-
                        <!-- start content -->
+
-
 
+
-
 
+
-
 
+
-
<style>
+
-
h1.firstHeading { display: none; }
+
-
 
+
-
p {text-align: justify;}
+
-
 
+
-
a:link { color: #004080; text-decoration: none}
+
-
a:visited { color:##003090; text-decoration: none}
+
-
a:hover { color:#f29400; text-decoration: none}
+
-
a:active { color:#f29400; text-decoration: none}
+
-
 
+
-
#bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
+
-
 
+
-
table#team_members { text-align: justify; border: 0; }
+
-
table#team_members h2, table#team_members h3 { clear: both; }
+
-
 
+
-
 
+
-
/*-----------------------------------------------------------------------------------------------*/
+
-
div.MenuBar ul li ul.DropDownMenu {
+
-
  display: none; /* Hides all drop-down menus. */
+
-
 
+
-
}
+
-
/*
+
-
li:hover works in IE7 and FF2.
+
-
a:hover works in IE6 and FF2.
+
-
a:hover breaks li:hover in FF2.
+
-
*/
+
-
div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
+
-
  display: none; /* Hides all side menus. */
+
-
}
+
-
/*------------------------------------------------------------------------------------- Menu Bar */
+
-
div.MenuBar {
+
-
  font: Verdana;
+
-
  height: 30px;
+
-
  width: 910px;
+
-
  /*width: 100%*/
+
-
  margin: 0;
+
-
  border-top: 0;
+
-
  border-right: 0;
+
-
  border-left: 0;
+
-
  padding: 0;
+
-
  background: #00446b;
+
-
 
+
-
}
+
-
div.MenuBar ul {
+
-
  font: Verdana;
+
-
  text-align: center;
+
-
  list-style-type: none;
+
-
  margin: 0 auto;
+
-
  border: 0;
+
-
  padding: 0;
+
-
  background: #00446b;
+
-
}
+
-
div.MenuBar ul li {
+
-
  font: Verdana;
+
-
  display: block;
+
-
  padding: 0;
+
-
  margin: 0;
+
-
  font-size: 1.3em;
+
-
  float: left;
+
-
  background: #00446b;
+
-
  text-align: center;
+
-
  width: 107px;
+
-
  position: relative; /* Sets the positioning context for each drop-down menu. */
+
-
}
+
-
 
+
-
div.MenuBar ul li a {
+
-
  font: Verdana;
+
-
  display: block;
+
-
  background: #00446b;
+
-
  height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
+
-
  color: white;
+
-
  padding-top: 4px;
+
-
  padding-bottom: 4px;
+
-
  padding-left: 1em; /* Sets the left space between top-level items. */
+
-
  padding-right: 1em; /* Sets the right space between top-level items. */
+
-
  text-decoration: none;
+
-
}
+
-
 
+
-
/*------------------------------------------------------------------------------ Drop-Down Menus */
+
-
div.MenuBar ul li:hover ul.DropDownMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu {
+
-
  display: block;
+
-
  width: 10em; /* Drop-down menu width.
+
-
                  Use MenuTailor.css to customize. */
+
-
  height: 1em;
+
-
  padding: 1px; /* Sets the drop-down menu "effective border" width. */
+
-
  position: absolute;
+
-
  top: 28px; /* Places the drop-down menu under the menu bar.
+
-
                Keep it sync with the menu bar height. */
+
-
  left: 0; /* Aligns the drop-down menu to its top-level item. */
+
-
  background-color: #A3C0E0; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
 
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a {
+
-
  width: 10em; /* Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
  height: 1em;
+
-
  padding-left: 0;
+
-
  padding-right: 0;
+
-
  background-color: #A3C0E0; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
}
+
-
ul.DropDownMenu li a span {
+
-
  display: block;
+
-
  padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
+
-
  padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
+
-
  text-align: right; /* Aligns the >> symbol to the right. */
+
-
}
+
-
ul.DropDownMenu li a span span {
+
-
  float: left; /* Aligns the text (back) to the left. */
+
-
  font: 12px Verdana; /* Required for IE55. */
+
-
  height: 20px;
+
-
  color: #FFFFFF;
+
-
}
+
-
/*----------------------------------------------------------------------------------- Side Menus */
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
+
-
  display: block;
+
-
  width: 11em; /* Side menu width.
+
-
                  Use MenuTailor.css to customize. */
+
-
  padding: 1px; /* Sets the side menu "effective border" width. */
+
-
  position: absolute;
+
-
  top: -1px; /* Aligns the side menu to its drop-down menu item.
+
-
                Keep it sync with the side menu "effective border" width. */
+
-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
+
-
  width: 11em; /* Keep it sync with the side menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
  font: 12px Verdana; /* Required for IE55. */
+
-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
}
+
-
div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
+
-
  padding-left: 1.5em; /* Sets the left space of each side menu item. */
+
-
  padding-right: 0.5em; /* Sets the right space of each side menu item. */
+
-
  text-align: left;
+
-
  font: 12px Verdana; /* Required for IE55. */
+
-
  left: 13em; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width.
+
-
                            Use MenuTailor.css to customize. */
+
-
}
+
-
/*----------------------------------------------------------------------------- Browser Specific */
+
-
* html div.MenuBar ul li a {
+
-
  float: left; /* Required for IE55 and IE6.
+
-
                            Breaks O9.
+
-
                            Hidden (* html) from non-IE browsers. */
+
-
}
+
-
* html ul.DropDownMenu li a:hover {
+
-
  cursor: hand; /* Required for IE55.
+
-
                  Hidden (* html) from non-IE browsers. */
+
-
}
+
-
ul.DropDownMenu li a:hover {
+
-
  cursor: pointer; /* Required for IE6 and IE7.
+
-
                      Hidding it (* html) from non-IE browsers breaks IE7!
+
-
}
+
-
* html div.MenuBar a:hover {
+
-
  text-decoration: none; /* Required for IE55 and IE6.
+
-
                            Hidden (* html) from non-IE browsers. */
+
-
}
+
-
* html div.MenuBar ul li table,
+
-
* html div.MenuBar ul li table td {
+
-
  border: 0; /* Required for IE55 and IE6.
+
-
                Hidden (* html) from non-IE browsers. */
+
-
}
+
-
/*------------------------------------------------------------------------------- Default Colors */
+
-
div.MenuBar {
+
-
  background-color: Menu;
+
-
  border-bottom: 1px solid ButtonShadow;
+
-
}
+
-
div.MenuBar a {
+
-
  background-color: Menu; /* Top-level unselected items. */
+
-
  color: MenuText;
+
-
}
+
-
div.MenuBar ul li:hover a,
+
-
div.MenuBar ul li a:hover {
+
-
  color: #A3C0E0;
+
-
  background-color: Highlight; /* Top-level selected item. */
+
-
  color: HighlightText;
+
-
}
+
-
/*...............................................................................................*/
+
-
div.MenuBar ul li:hover ul.DropDownMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu {
+
-
  background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a {
+
-
  background-color: Menu;  Drop-down menu unselected items.
+
-
                            Sets the drop-down menu "effective background" color. */
+
-
  color: MenuText;
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
+
-
  background-color: Highlight; /* Drop-down menu selected item. */
+
-
  color: HighlightText;
+
-
}
+
-
/*...............................................................................................*/
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
+
-
  background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
+
-
  background-color: Menu; /* Side menu unselected items.
+
-
                            Sets the side menu "effective background" color. */
+
-
  color: MenuText;
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
+
-
  background-color: Highlight; /* Side menu selected item. */
+
-
  color: HighlightText;
+
-
}
+
-
/*-----------------------------------------------------------------------------------------------*/
+
-
 
+
-
/*-------------------------------------------------------------------------------------- General */
+
-
body {
+
-
  background: white;
+
-
  color: black;
+
-
  margin: 0;
+
-
  border: 0;
+
-
  padding: 0;
+
-
}
+
-
 
+
-
 
+
-
div.MenuBar {
+
-
  font: 13px arial, helvetica, sans-serif;
+
-
}
+
-
div.MenuBar ul {
+
-
  font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
+
-
}
+
-
/*--------------------------------------------------------------------------------------- Colors */
+
-
div.MenuBar {
+
-
  background-color: #0064C8; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
  border-bottom: 1px solid ButtonShadow;
+
-
}
+
-
div.MenuBar a,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
+
-
  background-color: #00446b; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
}
+
-
div.MenuBar ul li:hover a,
+
-
div.MenuBar ul li a:hover,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
+
-
  background-color: #A3C0E0; /* Selected item. */
+
-
  color: #FFFFFF;
+
-
}
+
-
div.MenuBar ul li:hover ul.DropDownMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu,
+
-
div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
+
-
div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
+
-
  background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
+
-
}
+
-
/*--------------------------------------------------------------------------------------- Widths */
+
-
/*
+
-
 
+
-
/*
+
-
Menu Bar 1
+
-
Drop-Down Menu #2
+
-
*/
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
+
-
  width: 15.5em; /* Drop-down menu width. */
+
-
}
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
+
-
  width: 14em; /* Drop-down menu width. */
+
-
}
+
-
 
+
-
/*...............................................................................................*/
+
-
/*
+
-
Menu Bar 1
+
-
Drop-Down Menu #2
+
-
Side Menu #1
+
-
*/
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
+
-
  left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width. */
+
-
}
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
+
-
  width: 15em; /* Side menu width. */
+
-
}
+
-
/*...............................................................................................*/
+
-
/*
+
-
Menu Bar 1
+
-
Drop-Down Menu #2
+
-
Side Menu #2
+
-
*/
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
+
-
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width. */
+
-
}
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
+
-
  width: 7em; /* Side menu width. */
+
-
}
+
-
/*...............................................................................................*/
+
-
/*
+
-
Menu Bar 1
+
-
Drop-Down Menu #2
+
-
Side Menu #3
+
-
*/
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
+
-
  left: 15.5em  !important; /* Places the side menu to the right of the drop-down menu.
+
-
                            Keep it sync with the drop-down menu width. */
+
-
}
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
+
-
div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
+
-
div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
+
-
  width: 17em; /* Side menu width. */
+
-
}
+
-
/*...............................................................................................*/
+
-
 
+
-
</style>
+
-
 
+
-
 
+
-
 
+
-
</p><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
+
-
                <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
+
-
                                <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
+
-
                <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
+
-
                <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
+
-
                <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&amp;action=creativecommons" rel="meta">
+
-
<link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=rss">
+
-
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=atom">
+
-
 
+
-
 
+
-
<body class="mediawiki ns-0 ltr page-Team_Goettingen">
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
                        <!-- start content -->
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
<p></p><div id="header"><img src="http://www.patrickreinke.de/igem/headpicture_2.jpg" alt="Team Goettingen">
+
-
<br><br></p><div class="MenuBar" id="navi">
+
-
        <ul>
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen" style="color: white;">Home<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                        <!--<ul class="DropDownMenu" id="MB1-DDM6">
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Team"><span><span>News</span></span></a></li>
+
-
                        </ul>  -->
+
-
                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/Team" style="color: white;">Team<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                        <ul class="DropDownMenu" id="MB1-DDM5">
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Team"><span><span>Members</span></span></a></li>
+
-
                        <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#advisor"><span><span><div style="text-indent:20px;">&#8901; Advisors</div></span></span></a></li> -->
+
-
                        <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#students"><span><span><div style="text-indent:20px;">&#8901; Students</div></span></span></a></li> -->
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/Team#responsibitlities"><span><span><div style="text-indent:20px;">&#8901; Responsibilities</div></span></span></a></li> -->
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Focus_Groups"><span><span>Focus Groups</span></span></a></li>
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group1"><span><span><div style="text-indent:20px;">&#8901; #1 Selection / Swimming</div></span></span></a></li>  -->
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group2"><span><span><div style="text-indent:20px;">&#8901; #2 Speed Improvement</div></span></span></a></li>  -->
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group3"><span><span><div style="text-indent:20px;">&#8901; #3 Chemoreceptor</div></span></span></a></li>    -->
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#library"><span><span><div style="text-indent:20px;">&#8901; Library</div></span></span></a></li>            -->
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Seminars"><span><span>Seminars</span></span></a></li>
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#announce"><span><span><div style="text-indent:20px;">&#8901; Announcements</div></span></span></a></li>    -->
+
-
                        <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#presentations"><span><span><div style="text-indent:20px;">&#8901; Past Presentations</div></span></span></a></li> -->
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Work_Impressions"><span><span>Work Impressions</span></span></a></li>
+
-
                        </ul>
+
-
                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/Project" style="color: white;">Project<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                        <ul class="DropDownMenu" id="MB1-DDM2-SM3">
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Project"><span><span>Our Project</span></span></a></li>
+
-
                        <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Chemotaxis"><span><span><div style="text-indent:20px;">&#8901; Chemotaxis</div></span></span></a></li>  -->
+
-
                        <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Poster"><span><span><div style="text-indent:20px;">&#8901; Poster</div></span></span></a></li>    -->
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Materials"><span><span>Materials</span></span></a>
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Methods"><span><span>Methods</span></span></a>
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Computational_Data"><span><span>Computational Data</span></span></a>
+
-
                        <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">&#8901; Maps</div></span></span></a></li>
+
-
                        </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">&#8901; Sequences</div></span></span></a></li> -->
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Bioinformatical_Tool"><span><span>Bioinformatical Tools</span></span></a>
+
-
                        <li><a href="https://2012.igem.org/Team:Goettingen/Project/Lab_Work_Flow"><span><span>Lab Work Flow</span></span></a>
+
-
                        <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">&#8901; Golden Lab Rules</div></span></span></a></li>
+
-
                        </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">&#8901; Daily Check List</div></span></span></a></li> -->
+
-
                        </ul>
+
-
                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
 
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/Notebook" style="color: white;">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                                <ul class="DropDownMenu" id="MB1-DDM2">
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook"><span><span>Overview</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary"><span><span>Summary</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Discussion"><span><span>Discussion</span></span></a></li>
+
-
                                </ul>
+
-
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/iGEM" style="color: white;">iGEM<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                                <ul class="DropDownMenu" id="MB1-DDM6">
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM"><span><span>What is iGEM?</span></span></a></li>
+
-
                                <!--<li><a href="https://2012.igem.org/Team:Goettingen/BioBrick_System"><span><span>iGEM BioBrick System</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Part_Registry"><span><span>iGEM Part Registry</span></span></a></li>  -->
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts_Submitted"><span><span>Parts Submitted</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Attributions"><span><span>Attributions</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Saftey"><span><span>Saftey</span></span></a></li>>
+
-
                                </ul>
+
-
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/Goettingen" style="color: white;">G&ouml;ttingen<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                                <ul class="DropDownMenu" id="MB1-DDM5">
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen"><span><span>City</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/University"><span><span>Georg-August-University</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/MPI"><span><span>Max-Planck-Institute</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/S1-Demo_Lab"><span><span>S1-Demo Lab</span></span></a></li>
+
-
 
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                                        <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                                </li>
+
-
 
+
-
                                </ul>
+
-
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
                <li style="width: 160px">
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/Human_Practice" style="color: white;">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                                <ul class="DropDownMenu" id="MB1-DDM5">
+
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice"><span><span>Why Human Practice?</span></span></a></li>
+
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Public_and_Media"><span><span>Public and Media</span></span></a></li>
+
-
                              <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">&#8901; Newspaper</div></span></span></a></li>
+
-
                              </li><li><a href="https://2012.igem.org/Team:Goettingen/Conference"><span><span><div style="text-indent:20px;">&#8901; Conference</div></span></span></a></li>
+
-
                              </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">&#8901; Synthetic Biology Day</div></span></span></a></li> -->
+
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Survey"><span><span>Survey</span></span></a></li>
+
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion"><span><span>Panel Discussion</span></span></a></li>-->
+
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
+
-
                              </ul>
+
-
                                <!--[if lte IE 6]></td></tr></table></a><![endif]-->
+
-
                </li>
+
-
 
+
-
                <li>
+
-
                        <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
+
-
                        <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
+
-
                                <ul class="DropDownMenu" id="MB1-DDM2-SM3">
+
-
                              <li><a href="https://2012.igem.org/Team:Goettingen/Sponsoring"><span><span>Interested in...?</span></span></a></li>
+
-
                                <!-- <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Sponsors"><span><span>Sponsors</span></span></a></li>
+
-
                                <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Supporters"><span><span>Supporters</span></span></a></li>    -->
+
-
                </li>
+
-
        </ul>
+
-
 
+
-
</div>
+
-
 
+
-
 
+
-
<table>
+
-
                                <!-- Text body -->
+
-
<tbody><tr valign="top" align="left">
+
-
 
+
-
<td style="padding: 0pt 20px 0pt 0pt;" width="650px">
+
-
<font face="Verdana" size="-1">
+
-
<br>
+
-
Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
+
-
<br>
+
Line 531: Line 20:
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<h2><b>V09_17 </b></h2><br>
<h2><b>V09_17 </b></h2><br>
-
<b>V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and Psb1c3 followed by ligation</i></b><br>
+
<b>V09_17_1 Preparative double digestion of <i>flhDC</i>, <i>FliC</i> and pSB1C3 followed by ligation</i></b><br>
<ul>
<ul>
<li>Experiment: <br>  
<li>Experiment: <br>  
-
In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a> href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
+
In order to clone the <i>flhDC</i> and <i>FliC</i> into pSB1C3,  all components were digested with <i>EcoRI</i> and <i>PstI</i> according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated.<br>
-
        </ul>
+
</ul>
<br>
<br>
<b>V09_17_2 Preparation of over night cultures</b><br>
<b>V09_17_2 Preparation of over night cultures</b><br>
         <ul>
         <ul>
-
<li>Experiment: We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (Mg1655 & BL21) in order to test wether the motolity is influenced. The following constructs were used: <br>
+
<li>Experiment:<br>
-
- 20E_flhDC <br>
+
We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (MG1655 & BL21) in order to test wether the motility is influenced. The following constructs were used: <br>
-
- 18C_flhDC <br>
+
<br>
-
- 20I_flhdc <br>
+
- 20E_<i>flhDC</i> <br>
-
- 20E_motA <br>
+
- 18C_<i>flhDC</i> <br>
-
- 18C_motA <br>
+
- 20I_<i>flhdc</i> <br>
-
- 20E motB <br>
+
- 20E_<i>motA</i> <br>
-
- 18C_motB <br>
+
- 18C_<i>motA</i> <br>
-
Text</li>
+
- 20E_<i>motB</i> <br>
 +
- 18C_<i>motB</i> <br></li>
</li></ul>
</li></ul>
<br>
<br>
Line 558: Line 48:
<tr bordercolor="black" valign="top">
<tr bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
-
<h2><b>Versuchsnummer </b></h2><br>
+
<h2><b>V09_18 </b></h2><br>
-
<b> Titel</i></i></b><br>
+
<b> V09_18_1 Motility Assays</i></i></b><br>
<ul>
<ul>
-
<li>Experiment: <br>  
+
<li>Experiment: For the motility assay the over night cultures, with different constructs, were spun down and dropped onto M9- or 1% trypton-agar. The exact protocol you can find <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">HERE</a> <br>
-
Text<br>
+
</ul>
 +
<br>
 +
<b>V09_18_2 Preparative double digestion of <i>MotA</i>, <i>MotB</i>, <i>yhjH</i>, 18C-<i>RFP</i>, 20E-<i>RFP</i> and 20I-<i>RFP</i></b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
The genes of interest, motA, motB and yhjH were digested with <i>XbaI</i> and <i>PstI</i>. The plasmids with the different promoters were treated with <i>SpeI</i> and <i>PstI</i>. The digestion was then controlled via gel-electrophoresis.<br>
 +
<li> Observation and Results:<br>
 +
Since for all clones band of the expected size could be obtained these were isolated from the gel and purified using PeqGOLD Gelextraction Kit (Peqlab) according to the manual.<br>
 +
</ul>
 +
<br>
 +
<b>V09_18_3 Ligation of <i>MotA</i>, <i>MotB</i> and <i>yhjH</i> into 18C-<i>RFP</i>, 20E-<i>RFP</i> and 20I-<i>RFP</i></b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
<i>motA</i>, <i>motB</i> and <i>yhjH</i> were ligated into the plasmid pSB1C3 with different promoters (20E, 20I and 18C) according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.<br>
 +
</ul>
 +
<br>
 +
<b>V09_18_4 Chemical transformation of different plasmid-gene constructs into <i>E. coli</i> (DH10B) or (MG1655)</b><br>
 +
<ul>
 +
<li>Experiment:<br> Chemical transformation was done according to the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The following constructs were used:<br>
 +
<br>
 +
- pSB1C3-<i>FliC</i> into <i>E. coli </i>(DH10B)<br>
 +
- pSB1C3-<i>flhDC</i> into <i>E. coli </i>(DH10B)<br>
 +
- PSB1C3-<i>RFP</i> into <i>E. coli </i> (MG1655)<br>
 +
<br>
 +
<li>Observations & Results:<br>
 +
On all plates only a few colonies were observed.
 +
</ul>
 +
<br>
<br></td></tr>
<br></td></tr>
</table>
</table>
 +
 +
 +
<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 +
<tr bordercolor="black" valign="top">
 +
<td width="900" bordercolor="black" valign="top">
 +
<h2><b>V09_19 </b></h2><br>
 +
<b> V09_19_1 Motility Assays</i></i></b><br>
 +
<ul>
 +
<li>Experiment: For the motility assay the cultures with different constructs were grown for 6 hours. After that they were spun down and dropped onto M9- or 1% trypton-agar. The exact protocol you can find <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">HERE</a><br>
 +
The Following constructs in the <i>E. coli</>strain MG1655 were used:<br>
<br>
<br>
-
<body id="top">
+
-pSB1C3-20I-<i>flhDC</i><br>
-
<a href="#top">&uarr; Return to top</a>
+
-pSB1C3-18C-<i>flhDC</i><br>
 +
-pSB1C3-20E-<i>flhDC</i><br>
 +
-pSB1C3-<i>RFP</i><br>
<br>
<br>
 +
<li> Observation and Results:<br>
 +
At the following day (20th of september) the colonies still looked exactly the same. Neither chemotaxis nor swimming could be observed.<br>
 +
</ul>
<br>
<br>
-
</td>
+
<b>V09_19_2 Chemical transformation</b><br>
-
 
+
<ul>
-
 
+
<li>Experiment: <br>
-
<table bordercolor="black" border="1 px" width="600 px"><tr><td>
+
For the chemical transformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed. The following constructs were transformed:<br>
-
<b>Important pages</b>:<br>
+
<br>
-
<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
+
- pSB1C3-18C-<i>motA</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
+
- pSB1C3-18C-<i>motB</i> (DH10B)<br>
-
<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
+
- pSB1C3-18C-<i>yhjH</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
+
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
+
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
+
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
+
- pSB1C3-20I-<i>motA</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
+
- pSB1C3-20I-<i>motB</i> (DH10B)<br>
-
<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
+
- pSB1C3-20I-<i>yhjH</i> (DH10B)<br>
-
</td></tr>
+
- pSB1C3-<i>RFP</i> (BL21)<br>
 +
<br>
 +
</ul>
 +
<br>
 +
<b>V09_19_3 Preparation of over night cultures</b><br>
 +
<ul>
 +
<li>Experiment:<br>
 +
Over night cultures were prepared of three colonies of each constructs in order to isolate the plasmid and check the correct insert.<br>
 +
- pSB1C3-<i>flhDC</i> <br>
 +
- pSB1C3-<i>fliC</i> <br>
 +
<br>
 +
<br></td></tr>
</table>
</table>
Line 591: Line 134:
 +
<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 +
<tr bordercolor="black" valign="top">
 +
<td width="900" bordercolor="black" valign="top">
 +
<h2><b>V09_20 </b></h2><br>
 +
<b> V09_20_1 Mini Prep and test digestion of pSB1C3-gene constructs</i></i></b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. All constructs were digested with XbaI and SpeI conducted as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol. </a> <br>
 +
<br>
 +
- pSB1C3-<i>flhDC</i> <br>
 +
- pSB1C3-<i>fliC</i> <br>
 +
<br>
 +
<li> Observation and Results:<br>
 +
The pSB1C3-<i>flhDC / fliC</i> clones do not host the correctly inserted gene.<br>
 +
</ul>
 +
<br>
 +
<b>V09_20_2 PCR of <i>fliC</i></b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
In order to get more <i>fliC</i>, the already quikchanged <i>fliC</i> was amplified via PCR. The PCR product was analyzed via gel-electrophoresis and subsequently purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.<br>
 +
<li> Observation and Results:<br>
 +
The PCR was not succesful, we could not observe a band at the expected size.<br>
 +
</ul>
 +
<br>
 +
<b>V09_20_3 Repetition of preparative double digestion of <i>flhDC and pSB1C3</b><br>
 +
<ul>
 +
<li>Experiment:<br> In order to clone the <i>flhDC</i> into pSB1C3, all components were digested with <i>EcoRI</i> and </i>PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).<br>
 +
</ul>
 +
<br>
 +
<b>V09_20_4 Preparation of over night cultures</b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
Over night cultures were prepared of three colonies of each constructs in order to isolate the plasmid and check the correct insert.<br>
 +
<br>
 +
- pSB1C3-18C-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-18C-<i>motB</i> (DH10B)<br>
 +
- pSB1C3-18C-<i>yhjH</i> (DH10B)<br>
 +
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20I-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20I-<i>motB</i> (DH10B)<br>
 +
- pSB1C3-20I-<i>yhjH</i> (DH10B)<br>
 +
- pSB1C3-<i>RFP</i> (BL21)<br>
 +
<br></td></tr>
 +
</table>
 +
<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 +
<tr bordercolor="black" valign="top">
 +
<td width="900" bordercolor="black" valign="top">
 +
<h2><b>V09_21 </b></h2><br>
 +
<b> V09_21_1 Ligation and chemical Transformation of pSB1C3-<i>flhDC</i></b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
The restriction products were separated on a gel in order to verify the digestions success and purify the desired fragments. After ligation of the genes into pSB1C3, we transformed the ligated plasmids into <i>E. coli</i> DH10B according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br>
 +
<br>
 +
</ul>
 +
<br>
 +
<b>V09_21_2 Mini Prep and test digestion of pSB1C3-promoter-gene constructs</b><br>
 +
<ul>
 +
<li>Experiment: <br>
 +
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. All constructs were digested with <i>XbaI</i> and <i>SpeI</i> conducted as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>.<br>
 +
<br>
 +
- pSB1C3-18C-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-18C-<i>motB</i> (DH10B)<br>
 +
- pSB1C3-18C-<i>yhjH</i> (DH10B)<br>
 +
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20E-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20I-<i>motA</i> (DH10B)<br>
 +
- pSB1C3-20I-<i>motB</i> (DH10B)<br>
 +
- pSB1C3-20I-<i>yhjH</i> (DH10B)<br>
 +
- pSB1C3-<i>RFP</i> (BL21)<br>
 +
<br>
 +
<li> Observation and Results:<br>
 +
All clones host the correctly inserted gene in the plasmid.<br>
 +
</ul>
 +
<br>
 +
<br></td></tr>
 +
</table>
-
 
+
<br>
-
 
+
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
 +
<br>
 +
</td>
                                 <!-- Text body end-->
                                 <!-- Text body end-->
Line 602: Line 226:
</body>
</body>
</html>
</html>
 +
{{GoettingenFooter}}

Latest revision as of 23:27, 26 September 2012

Deutsch  / English 

#2 Speed Improvement - 21st week

Back to overview

V09_17


V09_17_1 Preparative double digestion of flhDC, FliC and pSB1C3 followed by ligation
  • Experiment:
    In order to clone the flhDC and FliC into pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated.

V09_17_2 Preparation of over night cultures
  • Experiment:
    We prepared overnight cultures of different promoter-gene constructs in two different E. coli strains (MG1655 & BL21) in order to test wether the motility is influenced. The following constructs were used:

    - 20E_flhDC
    - 18C_flhDC
    - 20I_flhdc
    - 20E_motA
    - 18C_motA
    - 20E_motB
    - 18C_motB


V09_18


V09_18_1 Motility Assays
  • Experiment: For the motility assay the over night cultures, with different constructs, were spun down and dropped onto M9- or 1% trypton-agar. The exact protocol you can find HERE

V09_18_2 Preparative double digestion of MotA, MotB, yhjH, 18C-RFP, 20E-RFP and 20I-RFP
  • Experiment:
    The genes of interest, motA, motB and yhjH were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. The digestion was then controlled via gel-electrophoresis.
  • Observation and Results:
    Since for all clones band of the expected size could be obtained these were isolated from the gel and purified using PeqGOLD Gelextraction Kit (Peqlab) according to the manual.

V09_18_3 Ligation of MotA, MotB and yhjH into 18C-RFP, 20E-RFP and 20I-RFP
  • Experiment:
    motA, motB and yhjH were ligated into the plasmid pSB1C3 with different promoters (20E, 20I and 18C) according to the protocol.

V09_18_4 Chemical transformation of different plasmid-gene constructs into E. coli (DH10B) or (MG1655)
  • Experiment:
    Chemical transformation was done according to the standard protocol. The following constructs were used:

    - pSB1C3-FliC into E. coli (DH10B)
    - pSB1C3-flhDC into E. coli (DH10B)
    - PSB1C3-RFP into E. coli (MG1655)

  • Observations & Results:
    On all plates only a few colonies were observed.


V09_19


V09_19_1 Motility Assays
  • Experiment: For the motility assay the cultures with different constructs were grown for 6 hours. After that they were spun down and dropped onto M9- or 1% trypton-agar. The exact protocol you can find HERE
    The Following constructs in the E. colistrain MG1655 were used:

    -pSB1C3-20I-flhDC
    -pSB1C3-18C-flhDC
    -pSB1C3-20E-flhDC
    -pSB1C3-RFP

  • Observation and Results:
    At the following day (20th of september) the colonies still looked exactly the same. Neither chemotaxis nor swimming could be observed.

V09_19_2 Chemical transformation
  • Experiment:
    For the chemical transformation the standard protocol was followed. The following constructs were transformed:

    - pSB1C3-18C-motA (DH10B)
    - pSB1C3-18C-motB (DH10B)
    - pSB1C3-18C-yhjH (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20I-motA (DH10B)
    - pSB1C3-20I-motB (DH10B)
    - pSB1C3-20I-yhjH (DH10B)
    - pSB1C3-RFP (BL21)


V09_19_3 Preparation of over night cultures
  • Experiment:
    Over night cultures were prepared of three colonies of each constructs in order to isolate the plasmid and check the correct insert.
    - pSB1C3-flhDC
    - pSB1C3-fliC


V09_20


V09_20_1 Mini Prep and test digestion of pSB1C3-gene constructs
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. All constructs were digested with XbaI and SpeI conducted as described in the protocol.

    - pSB1C3-flhDC
    - pSB1C3-fliC

  • Observation and Results:
    The pSB1C3-flhDC / fliC clones do not host the correctly inserted gene.

V09_20_2 PCR of fliC
  • Experiment:
    In order to get more fliC, the already quikchanged fliC was amplified via PCR. The PCR product was analyzed via gel-electrophoresis and subsequently purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.
  • Observation and Results:
    The PCR was not succesful, we could not observe a band at the expected size.

V09_20_3 Repetition of preparative double digestion of flhDC and pSB1C3
  • Experiment:
    In order to clone the flhDC into pSB1C3, all components were digested with EcoRI and     PstI according to the protocol. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).

V09_20_4 Preparation of over night cultures
  • Experiment:
    Over night cultures were prepared of three colonies of each constructs in order to isolate the plasmid and check the correct insert.

    - pSB1C3-18C-motA (DH10B)
    - pSB1C3-18C-motB (DH10B)
    - pSB1C3-18C-yhjH (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20I-motA (DH10B)
    - pSB1C3-20I-motB (DH10B)
    - pSB1C3-20I-yhjH (DH10B)
    - pSB1C3-RFP (BL21)

V09_21


V09_21_1 Ligation and chemical Transformation of pSB1C3-flhDC
  • Experiment:
    The restriction products were separated on a gel in order to verify the digestions success and purify the desired fragments. After ligation of the genes into pSB1C3, we transformed the ligated plasmids into E. coli DH10B according to the protocol.


V09_21_2 Mini Prep and test digestion of pSB1C3-promoter-gene constructs
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. All constructs were digested with XbaI and SpeI conducted as described in the protocol.

    - pSB1C3-18C-motA (DH10B)
    - pSB1C3-18C-motB (DH10B)
    - pSB1C3-18C-yhjH (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20E-motA (DH10B)
    - pSB1C3-20I-motA (DH10B)
    - pSB1C3-20I-motB (DH10B)
    - pSB1C3-20I-yhjH (DH10B)
    - pSB1C3-RFP (BL21)

  • Observation and Results:
    All clones host the correctly inserted gene in the plasmid.



Back to overview
↑ Back to top!

Retrieved from "http://2012.igem.org/Team:Goettingen/week21-2"