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- | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
| + | {{GoettingenHeader|deu=Team:Goettingen/week16|eng=Team:Goettingen/week16}} |
- | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
| + | <html> |
- | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
| + | <table> |
- | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
| + | |
- | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
| + | |
- | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
| + | |
- | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss">
| + | |
- | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
| + | |
- | | + | |
- | | + | |
- | <body class="mediawiki ns-0 ltr page-Team_Goettingen">
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | <!-- start content -->
| + | |
- | | + | |
- | | + | |
- | | + | |
- | <style>
| + | |
- | h1.firstHeading { display: none; }
| + | |
- | | + | |
- | p {text-align: justify;}
| + | |
- | | + | |
- | a:link { color: #004080; text-decoration: none}
| + | |
- | a:visited { color:##003090; text-decoration: none}
| + | |
- | a:hover { color:#f29400; text-decoration: none}
| + | |
- | a:active { color:#f29400; text-decoration: none}
| + | |
- | | + | |
- | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
| + | |
- | | + | |
- | table#team_members { text-align: justify; border: 0; }
| + | |
- | table#team_members h2, table#team_members h3 { clear: both; }
| + | |
- | | + | |
- | | + | |
- | /*-----------------------------------------------------------------------------------------------*/
| + | |
- | div.MenuBar ul li ul.DropDownMenu {
| + | |
- | display: none; /* Hides all drop-down menus. */
| + | |
- | | + | |
- | }
| + | |
- | /*
| + | |
- | li:hover works in IE7 and FF2.
| + | |
- | a:hover works in IE6 and FF2.
| + | |
- | a:hover breaks li:hover in FF2.
| + | |
- | */
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| + | |
- | display: none; /* Hides all side menus. */
| + | |
- | }
| + | |
- | /*------------------------------------------------------------------------------------- Menu Bar */
| + | |
- | div.MenuBar {
| + | |
- | font: Verdana;
| + | |
- | height: 30px;
| + | |
- | width: 910px;
| + | |
- | /*width: 100%*/
| + | |
- | margin: 0;
| + | |
- | border-top: 0;
| + | |
- | border-right: 0;
| + | |
- | border-left: 0;
| + | |
- | padding: 0;
| + | |
- | background: #00446b;
| + | |
- | | + | |
- | }
| + | |
- | div.MenuBar ul {
| + | |
- | font: Verdana;
| + | |
- | text-align: center;
| + | |
- | list-style-type: none;
| + | |
- | margin: 0 auto;
| + | |
- | border: 0;
| + | |
- | padding: 0;
| + | |
- | background: #00446b;
| + | |
- | }
| + | |
- | div.MenuBar ul li {
| + | |
- | font: Verdana;
| + | |
- | display: block;
| + | |
- | padding: 0;
| + | |
- | margin: 0;
| + | |
- | font-size: 1.3em;
| + | |
- | float: left;
| + | |
- | background: #00446b;
| + | |
- | text-align: center;
| + | |
- | width: 107px;
| + | |
- | position: relative; /* Sets the positioning context for each drop-down menu. */
| + | |
- | }
| + | |
- | | + | |
- | div.MenuBar ul li a {
| + | |
- | font: Verdana;
| + | |
- | display: block;
| + | |
- | background: #00446b;
| + | |
- | height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
| + | |
- | color: white;
| + | |
- | padding-top: 4px;
| + | |
- | padding-bottom: 4px;
| + | |
- | padding-left: 1em; /* Sets the left space between top-level items. */
| + | |
- | padding-right: 1em; /* Sets the right space between top-level items. */
| + | |
- | text-decoration: none;
| + | |
- | }
| + | |
- | | + | |
- | /*------------------------------------------------------------------------------ Drop-Down Menus */
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
- | display: block;
| + | |
- | width: 10em; /* Drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | height: 1em;
| + | |
- | padding: 1px; /* Sets the drop-down menu "effective border" width. */
| + | |
- | position: absolute;
| + | |
- | top: 28px; /* Places the drop-down menu under the menu bar.
| + | |
- | Keep it sync with the menu bar height. */
| + | |
- | left: 0; /* Aligns the drop-down menu to its top-level item. */
| + | |
- | background-color: #A3C0E0; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | | + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
- | width: 10em; /* Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | height: 1em;
| + | |
- | padding-left: 0;
| + | |
- | padding-right: 0;
| + | |
- | background-color: #A3C0E0; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | ul.DropDownMenu li a span {
| + | |
- | display: block;
| + | |
- | padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
| + | |
- | padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
| + | |
- | text-align: right; /* Aligns the >> symbol to the right. */
| + | |
- | }
| + | |
- | ul.DropDownMenu li a span span {
| + | |
- | float: left; /* Aligns the text (back) to the left. */
| + | |
- | font: 12px Verdana; /* Required for IE55. */
| + | |
- | height: 20px;
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | /*----------------------------------------------------------------------------------- Side Menus */
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
- | display: block;
| + | |
- | width: 11em; /* Side menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | padding: 1px; /* Sets the side menu "effective border" width. */
| + | |
- | position: absolute;
| + | |
- | top: -1px; /* Aligns the side menu to its drop-down menu item.
| + | |
- | Keep it sync with the side menu "effective border" width. */
| + | |
- | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
- | width: 11em; /* Keep it sync with the side menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | font: 12px Verdana; /* Required for IE55. */
| + | |
- | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | }
| + | |
- | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| + | |
- | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| + | |
- | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| + | |
- | text-align: left;
| + | |
- | font: 12px Verdana; /* Required for IE55. */
| + | |
- | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | }
| + | |
- | /*----------------------------------------------------------------------------- Browser Specific */
| + | |
- | * html div.MenuBar ul li a {
| + | |
- | float: left; /* Required for IE55 and IE6.
| + | |
- | Breaks O9.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | * html ul.DropDownMenu li a:hover {
| + | |
- | cursor: hand; /* Required for IE55.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | ul.DropDownMenu li a:hover {
| + | |
- | cursor: pointer; /* Required for IE6 and IE7.
| + | |
- | Hidding it (* html) from non-IE browsers breaks IE7!
| + | |
- | }
| + | |
- | * html div.MenuBar a:hover {
| + | |
- | text-decoration: none; /* Required for IE55 and IE6.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | * html div.MenuBar ul li table,
| + | |
- | * html div.MenuBar ul li table td {
| + | |
- | border: 0; /* Required for IE55 and IE6.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | /*------------------------------------------------------------------------------- Default Colors */
| + | |
- | div.MenuBar {
| + | |
- | background-color: Menu;
| + | |
- | border-bottom: 1px solid ButtonShadow;
| + | |
- | }
| + | |
- | div.MenuBar a {
| + | |
- | background-color: Menu; /* Top-level unselected items. */
| + | |
- | color: MenuText;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover a,
| + | |
- | div.MenuBar ul li a:hover {
| + | |
- | color: #A3C0E0;
| + | |
- | background-color: Highlight; /* Top-level selected item. */
| + | |
- | color: HighlightText;
| + | |
- | }
| + | |
- | /*...............................................................................................*/
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
- | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
- | background-color: Menu; Drop-down menu unselected items.
| + | |
- | Sets the drop-down menu "effective background" color. */
| + | |
- | color: MenuText;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| + | |
- | background-color: Highlight; /* Drop-down menu selected item. */
| + | |
- | color: HighlightText;
| + | |
- | }
| + | |
- | /*...............................................................................................*/
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
- | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
- | background-color: Menu; /* Side menu unselected items.
| + | |
- | Sets the side menu "effective background" color. */
| + | |
- | color: MenuText;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
- | background-color: Highlight; /* Side menu selected item. */
| + | |
- | color: HighlightText;
| + | |
- | }
| + | |
- | /*-----------------------------------------------------------------------------------------------*/
| + | |
- | | + | |
- | /*-------------------------------------------------------------------------------------- General */
| + | |
- | body {
| + | |
- | background: white;
| + | |
- | color: black;
| + | |
- | margin: 0;
| + | |
- | border: 0;
| + | |
- | padding: 0;
| + | |
- | }
| + | |
- | | + | |
- | | + | |
- | div.MenuBar {
| + | |
- | font: 13px arial, helvetica, sans-serif;
| + | |
- | }
| + | |
- | div.MenuBar ul {
| + | |
- | font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
| + | |
- | }
| + | |
- | /*--------------------------------------------------------------------------------------- Colors */
| + | |
- | div.MenuBar {
| + | |
- | background-color: #0064C8; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | border-bottom: 1px solid ButtonShadow;
| + | |
- | }
| + | |
- | div.MenuBar a,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
- | background-color: #00446b; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover a,
| + | |
- | div.MenuBar ul li a:hover,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
- | background-color: #A3C0E0; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
- | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| + | |
- | }
| + | |
- | /*--------------------------------------------------------------------------------------- Widths */
| + | |
- | /*
| + | |
- | | + | |
- | /*
| + | |
- | Menu Bar 1
| + | |
- | Drop-Down Menu #2
| + | |
- | */
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
| + | |
- | width: 15.5em; /* Drop-down menu width. */
| + | |
- | }
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
| + | |
- | width: 14em; /* Drop-down menu width. */
| + | |
- | }
| + | |
- | | + | |
- | /*...............................................................................................*/
| + | |
- | /*
| + | |
- | Menu Bar 1
| + | |
- | Drop-Down Menu #2
| + | |
- | Side Menu #1
| + | |
- | */
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
| + | |
- | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width. */
| + | |
- | }
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
| + | |
- | width: 15em; /* Side menu width. */
| + | |
- | }
| + | |
- | /*...............................................................................................*/
| + | |
- | /*
| + | |
- | Menu Bar 1
| + | |
- | Drop-Down Menu #2
| + | |
- | Side Menu #2
| + | |
- | */
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
| + | |
- | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width. */
| + | |
- | }
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
| + | |
- | width: 7em; /* Side menu width. */
| + | |
- | }
| + | |
- | /*...............................................................................................*/
| + | |
- | /*
| + | |
- | Menu Bar 1
| + | |
- | Drop-Down Menu #2
| + | |
- | Side Menu #3
| + | |
- | */
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
| + | |
- | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width. */
| + | |
- | }
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
| + | |
- | width: 17em; /* Side menu width. */
| + | |
- | }
| + | |
- | /*...............................................................................................*/
| + | |
- | | + | |
- | </style>
| + | |
- | | + | |
- | | + | |
- | | + | |
- | </p><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
| + | |
- | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
| + | |
- | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
| + | |
- | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
| + | |
- | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
| + | |
- | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
| + | |
- | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss">
| + | |
- | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
| + | |
- | | + | |
- | | + | |
- | <body class="mediawiki ns-0 ltr page-Team_Goettingen">
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- | <p></p><div id="header"><img src="http://www.patrickreinke.de/igem/headpicture_2.jpg" alt="Team Goettingen">
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Team"><span><span>Members</span></span></a></li>
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- | <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#advisor"><span><span><div style="text-indent:20px;">⋅ Advisors</div></span></span></a></li> -->
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- | <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#students"><span><span><div style="text-indent:20px;">⋅ Students</div></span></span></a></li> -->
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- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/Team#responsibitlities"><span><span><div style="text-indent:20px;">⋅ Responsibilities</div></span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Focus_Groups"><span><span>Focus Groups</span></span></a></li>
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- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group1"><span><span><div style="text-indent:20px;">⋅ #1 Selection / Swimming</div></span></span></a></li> -->
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- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group2"><span><span><div style="text-indent:20px;">⋅ #2 Speed Improvement</div></span></span></a></li> -->
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- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group3"><span><span><div style="text-indent:20px;">⋅ #3 Chemoreceptor</div></span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Seminars"><span><span>Seminars</span></span></a></li>
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- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#announce"><span><span><div style="text-indent:20px;">⋅ Announcements</div></span></span></a></li> -->
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- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#presentations"><span><span><div style="text-indent:20px;">⋅ Past Presentations</div></span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Work_Impressions"><span><span>Work Impressions</span></span></a></li>
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- | <a href="https://2012.igem.org/Team:Goettingen/Project" style="color: white;">Project<!--[if gt IE 6]><!--></a><!--<![endif]-->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Project"><span><span>Our Project</span></span></a></li>
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- | <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Chemotaxis"><span><span><div style="text-indent:20px;">⋅ Chemotaxis</div></span></span></a></li> -->
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- | <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Poster"><span><span><div style="text-indent:20px;">⋅ Poster</div></span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Materials"><span><span>Materials</span></span></a>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Methods"><span><span>Methods</span></span></a>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Computational_Data"><span><span>Computational Data</span></span></a>
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- | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">⋅ Maps</div></span></span></a></li>
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- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">⋅ Sequences</div></span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Bioinformatical_Tool"><span><span>Bioinformatical Tools</span></span></a>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Lab_Work_Flow"><span><span>Lab Work Flow</span></span></a>
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- | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">⋅ Golden Lab Rules</div></span></span></a></li>
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- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">⋅ Daily Check List</div></span></span></a></li> -->
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- | <a href="https://2012.igem.org/Team:Goettingen/Notebook" style="color: white;">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook"><span><span>Overview</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary"><span><span>Summary</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Discussion"><span><span>Discussion</span></span></a></li>
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- | <a href="https://2012.igem.org/Team:Goettingen/iGEM" style="color: white;">iGEM<!--[if gt IE 6]><!--></a><!--<![endif]-->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM"><span><span>What is iGEM?</span></span></a></li>
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- | <!--<li><a href="https://2012.igem.org/Team:Goettingen/BioBrick_System"><span><span>iGEM BioBrick System</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Part_Registry"><span><span>iGEM Part Registry</span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts_Submitted"><span><span>Parts Submitted</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Attributions"><span><span>Attributions</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Saftey"><span><span>Saftey</span></span></a></li>>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen"><span><span>City</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/University"><span><span>Georg-August-University</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/MPI"><span><span>Max-Planck-Institute</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/S1-Demo_Lab"><span><span>S1-Demo Lab</span></span></a></li>
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- | <li style="width: 160px">
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- | <a href="https://2012.igem.org/Team:Goettingen/Human_Practice" style="color: white;">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice"><span><span>Why Human Practice?</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Public_and_Media"><span><span>Public and Media</span></span></a></li>
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- | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">⋅ Newspaper</div></span></span></a></li>
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- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Conference"><span><span><div style="text-indent:20px;">⋅ Conference</div></span></span></a></li>
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- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">⋅ Synthetic Biology Day</div></span></span></a></li> -->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Survey"><span><span>Survey</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion"><span><span>Panel Discussion</span></span></a></li>-->
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
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- | </ul>
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- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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- | </li>
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- | <li>
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- | <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
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- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | <ul class="DropDownMenu" id="MB1-DDM2-SM3">
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Sponsoring"><span><span>Interested in...?</span></span></a></li>
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- | <!-- <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Sponsors"><span><span>Sponsors</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Supporters"><span><span>Supporters</span></span></a></li> -->
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- | </li>
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- | </ul>
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- | </div>
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- | <!-- Text body -->
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- | <font face="Verdana" size="-1">
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- | <br>
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- | Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
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| <ul> | | <ul> |
| <li>Experiment: <br> | | <li>Experiment: <br> |
- | In order to perform another test using the methionine containing agar and to determine the effect of <i>tar</i> new over night cultures of <i>tar</i>-18C and RFP-18C in Δ<i>tar</i> as well as MG1655 and RP437 without any construct were prepared. <br></li> | + | In order to perform another test we used methionine containing agar and determined the effect of new over night cultures of <i>tar</i>-18C and RFP-18C in Δ<i>tar</i> as well as MG1655 and RP437 without any construct. <br></li> |
| <br></td></tr> | | <br></td></tr> |
| </table> | | </table> |
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| <ul> | | <ul> |
| <li>Experiment: <br> | | <li>Experiment: <br> |
- | This time methionine was added to the prepared M9 agar plates and the Whatman Filter Paper were imbued with an amino acid mixture solution as an attractant. Here again the over night cultures were dropped onto the plates directly. <br></li> | + | This time methionine was added to the prepared M9 agar plates and the Whatman Filter Paper were imbued with an amino acid mixture solution as attractant. Here again the over night cultures were dropped onto the plates directly. <br></li> |
| <li>Observations & Results: <br> | | <li>Observations & Results: <br> |
- | The following day (14th August) already swimming could be observed on all plates. In general MG1655 featured the strongest motility, followed by <i>tar</i>-18C. This pattern intensified over the following days, but chemotactic behavior could not clearly be identified at all.</i></ul> | + | The following day (14th August) already swimming could be observed on all plates. In general MG1655 featured the strongest motility, followed by <i>tar</i>-18C. This pattern intensified over the following days, but at all no chemotactic behavior could clearly be identified.</i></ul> |
| <br> | | <br> |
| <b>V08_13_2 Preparation of over night cultures </b><br> | | <b>V08_13_2 Preparation of over night cultures </b><br> |
| <ul> | | <ul> |
| <li>Experiment: <br> | | <li>Experiment: <br> |
- | For we aimed to test <i>fliC</i> (DH10B), <i>fliC</i> (<i>Salmonella</i>), <i>motA</i>, <i>motB</i> and <i>yhjH</i> again, over night cultures of the constructs in <i>E. coli</i> DH10B, BL21, DH5α and XL1 Blue were prepared. Furthermore, cultures of pUC18 in the different strains as a reference were made.<br> | + | For testing <i>fliC</i> (DH10B), <i>fliC</i> (<i>Salmonella</i>), <i>motA</i>, <i>motB</i> and <i>yhjH</i> again, over night cultures of the constructs in <i>E. coli</i> DH10B, BL21, DH5α and XL1 Blue were prepared. Furthermore, cultures of pUC18 in the different strains as a reference were made.<br> |
| </ul><br> | | </ul><br> |
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- | | + | <h2><b>V08_16 </b></h2><br> |
- | <h2><b>V08_02 </b></h2><br> | + | <b> Preparative double digestion of 18M-<i>flhDC</i>, 18O-<i>flhDC</i> and 18C-<i>flhDC</i> </b><br> |
- | <b>V08_02_1 Performance of motility assay </b><br> | + | |
| <ul> | | <ul> |
| <li>Experiment: <br> | | <li>Experiment: <br> |
- | LB medium containing ampicillin was inoculated with the over night culture and shaken until an optical density of 0.4-0.6 was reached. Meanwhile ampicillin containing M9 agar plates were prepared and autoclaved Whatman Filter Papers were imbued with tryptone solution. Then the cultures were dropped on the agar at a distance of 1 cm to the paper. On each plate also colonies hosting the empty vector pUC18 were dropped in order to have a reference. Two drops were rather applied into the agar than onto as done before, because some paper predicted faster motility. Also this time each plate was prepared in duplicates.<br></li>
| + | The <i>flhDC</i>-promoter constructs were digested with <i>XbaI</i> and <i>PstI</i> as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Afterwards, the fragments of the desired size were cut out and frozen in order to extract the DNA from the gel slices the following day. <br></li> |
| <li>Observations & Results: <br> | | <li>Observations & Results: <br> |
- | Unfortunately, even after a week no swimming could be observed only growth. We assume that the medium might not provide adequate swimming conditions and therefore we plan to test more motile strains and alter the agar composition if necessary. </li></ul>
| + | The gel featured clearly visible bands of the expected sizes when irradiated with UV rays.</li> |
- | <br> | + | <br></td></tr> |
- | <b>V08_02_2 Preparation of over night cultures </b><br>
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- | <ul>
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- | <li>Experiment: <br>
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- | Since we recently received new <i>E. coli</i> strains that are more motile than our usually used laboratory stains new over night cultures of MG1655 and RP437 were prepared.<br>
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- | </ul><br>
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- | </td></tr> | + | |
| </table> | | </table> |
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- | <h2><b>V08_03 </b></h2><br> | + | <h2><b>V08_17 </b></h2><br> |
- | <b> Performance of motility assay </b><br> | + | <b> Purification of the <i>flhDC</i>-promoter constructs </b><br> |
| <ul> | | <ul> |
| <li>Experiment: <br> | | <li>Experiment: <br> |
- | This time the over night cultures were directly dropped to the swimming plates. In oder to test whether the medium or indeed our strains are responsible for the immobility of the bacteria the habitual M9 agar plates were prepared. However not antibiotics were used since the new strains are not equipped with any resistance yet. Then the autoclaved Whatman Filter Papers were imbued with tryptone solution were placed in the middle of the plate and the cultures were dropped on the agar at a distance of 1 cm to the paper. Also this time each plate was prepared in duplicates.<br></li>
| + | The restriction products were purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.<br></li> |
- | <li>Observations & Results: <br>
| + | |
- | The following day (4th of August) no motility could be observed. Since both strains are known to move very quick and are supposed to swarm the whole plate during hours we suggest that the medium composition is indeed not suitable. However, two days after preparation of the swimming assay (5th of August) some evidence for incipient swimming was visible, which grew to small halos the following days. Nevertheless, the slow progress confirmed our resolution to reconsider the composition of the plates. </i>
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| </table> | | </table> |
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- | <a href="#top">↑ Return to top</a>
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| <br> | | <br> |
| + | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> |
| <br> | | <br> |
| </td> | | </td> |
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- | <b>Important pages</b>:<br>
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- | <a href="https://2012.igem.org/Team:Goettingen">Home</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
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- | <a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
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- | </td></tr>
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