Team:HokkaidoU Japan/Safety

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1.<br />
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Would the materials used in your project and/or your final product pose:<br />
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Risks to the safety and health of team members or others in the lab?<br />
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Risks to the safety and health of the general public if released by design or accident?<br />
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Risks to environmental quality if released by design or accident?<br />
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Risks to security through malicious misuse by individuals, groups or states?<br />
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<h2>Safety Questions</h2>
 +
<div class="hokkaidou-section">
 +
<dl>
 +
<dt><h3>
 +
Risks to the safety and health of team members or others in the lab
 +
</h3></dt>
<dd>
<dd>
-
Safety and health of researchers:<br />
+
<p>
-
in the method of bioplastic polymer extraction, the use of chloroform may have the risk of injury. This risk can be avoided by the use of gloves and eye glasses.
+
All lab staff is trained according to safety manual provided by Hokkaido University. We took on
-
</dd>
+
ourselves to compile a shortlist of often used dangerous materials and safety procedures in our
 +
project.
 +
</p>
-
<dd>
+
<h4>Dangerous chemicals</h4>
-
Public safety:<br />
+
<p>
 +
<ul>
 +
<li>Chloroform - corrosive and toxic: must be used in fume hood</li>
 +
<li>Ethidium Bromide - intercalating agent: must be used with personal safety gear</li>
 +
<li>Ethanol - flammable: must not be used near open flame or in large quantities/li>
 +
<li>Liquid Nitrogen - cryogenic container and cryogenic gloves must be used</li></ul>
 +
</p>
 +
<h4>
 +
Procedures and equipment
 +
</h4>
 +
<p>
 +
<ul>
 +
<li>
 +
Agarose gel production -
 +
heating in sealed container (rupture risk), scalding hot and vicious during preparation
 +
(burn injury risk) - remove container lid before heating in microwave, use safety gear,
 +
wait for a few moments before removing from microwave</li>
 +
<li>
 +
Benson burner - fire risk: DO NOT use flammable materials especially ethanol near open
 +
fire</li><li>
 +
Centrifuge - high velocity: balance appropriately, observe the machine till it reaches top
 +
velocity</li><li>
 +
Autoclave - high pressure: check the water level, DO NOT open when pressurized
 +
</li><li>UV radiation - damage to eyes and skin: use glove and UV box or UV shield</li></ul>
 +
</p>
 +
<h4>
 +
Non-pathogenic bacteria (policy requires treating as pathogenic, as precaution)
 +
</h4>
 +
<p>
 +
<ul><li>JM109
 +
</li><li>DH5alpha</li></ul>
 +
Both of these are lab safe strains. As a precaution all materials coming in contact are sterilized
 +
before and after.
 +
</p>
 +
<i>Reference Federal Register, (1986) Vol. V1: 88, 6952–16985</i>
 +
<h4>
 +
Safety equipment
 +
</h4>
 +
<ul>
 +
<li>Gloves</li>
 +
<li>Coats</li>
 +
<li>Goggles</li>
 +
<li>UV Box</li>
 +
<li>UV shield</li>
 +
</ul>
 +
<h4>
 +
Waste disposal and sterilization
 +
</h4>
 +
<p>
 +
All equipment and waste coming in contact with bacterial is sterilized by autoclave or
 +
bleach. All chemicals compounds were disposed according to requirements for their disposal.
 +
All table surface used for work were sterilized with 70% ethanol before and after a procedure
 +
</p>
 +
<h4>
 +
Chemical Usage
 +
</h4>
 +
<p>
 +
All chemical compounds were used according to their manuals and respective material
 +
safety data sheet
 +
</p>
 +
<h4>
 +
Genetic material
 +
</h4>
 +
<p>
 +
All genes used in this project come from non-pathogenic bacterial strains of E. coli or R.
 +
eutropha. Expressed proteins did not show any toxic effect to their host. Our biobricks do not
 +
have any foreseeable selective advantage if released to the environment. After consideration
 +
we could not find any usage pausing a security concern.
 +
</p>
</dd>
</dd>
-
<dd>
 
-
Environmental safety:<br />
 
-
Cleaning and disposing of bacteria,bioproducts,wastes, are needed by special infrastructure.
 
-
Bioplastics (P3HB) which we are going to submit, are biodegradable plastics.
 
-
The study of bioplastics will lead us to a oil resource free society. 
 
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</dd>
 
-
 
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<dt>
 
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2.<br />
 
-
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,<br />
 
-
did you document these issues in the Registry?<br />
 
-
how did you manage to handle the safety issue?<br />
 
-
how could other teams learn from your experience?
 
-
</dt>
 
 +
<dt><h3>
 +
Risks to the safety and health of the general public if released by design or accident.
 +
</h3></dt>
<dd>
<dd>
-
The parts we submitted this year does not have safety risks according to professional judgment.
+
Also risks to environmental quality if released by design or accident.
 +
All material in the lab are kept safe and accessible only to the authorized lab members. All
 +
safety precaution were taken for waste disposal. Preventive actions as described previously
 +
were taken make sure the bacteria won’t be released into environment. In unlikely event of
 +
release bacteria is lab safe and doesn’t have selective advantage to propagate in nature. This
 +
bacteria and/or our biobrick are not designed for usage in public.
</dd>
</dd>
-
 
+
<dt><h3>
-
 
+
Risks to security through malicious misuse by individuals, groups or states. And do any
-
<dt>
+
of the new BioBrick parts (or devices) that you made this year raise any safety issues?
-
3.<br />
+
</h3></dt>
-
Is there a local bio safety group, committee, or review board at your institution?<br />
+
-
If yes, what does your local bio safety group think about your project?
+
-
</dt>
+
-
 
+
<dd>
<dd>
-
We have "the Safety Office of Genetic Recombination in Hokkaido University".
+
No biobricks made by us raise any safety issues. As described before all genes used in this
-
Our lab is equipped appropriately for the manipulation and genetic recombination of bacterial cells.
+
project come from non-pathogenic bacterial strains of E. coli or R. eutropha. Expressed proteins
-
Team members are instructed according to the safety training manual.
+
did not show any toxic effect to their host. Our biobricks do not have any foreseeable selective
 +
advantage if released to the environment. After consideration we could not find any usage
 +
pausing a security concern. Bacterial strains are not pathogenic and lab safe.
</dd>
</dd>
-
 
+
<dt><h3>
-
<dt>
+
Is there a local bio safety group, committee, or review board at your institution? If yes,
-
4.<br />
+
what does your local bio safety group think about your project?
-
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions?<br />
+
</h3></dt>
-
How could parts, devices and systems be made even safer through biosafety engineering?
+
-
</dt>
+
-
 
+
<dd>
<dd>
-
Our team suggests that users should always know the safety risks and the rules of of the parts, devices, and systems.<br />
+
Project conducted at Hokkaido University involving recombination are overseen by officer
-
We should always keep in mind to take a look of the safety page related to the parts that you are going to use.<br />
+
of "the Safety Office of Genetic Recombination in Hokkaido University". Our lab is equipped
-
Our suggestion are very simple, but as long as you are a scientist and an iGEM members, it can not be forgotten.
+
appropriately for the manipulation and genetic recombination of bacterial cells. Team members
 +
are instructed according to the safety training manual. Permission to conduct our experiments
 +
was acquired.
</dd>
</dd>
-
 
+
<dt><h3>
 +
Do you have any other ideas how to deal with safety issues that could be useful for
 +
future iGEM competitions? How could parts, devices and systems be made even safer
 +
through biosafety engineering?
 +
</h3></dt>
<dd>
<dd>
-
Genetically engineered bacteria may accidentally spread out to the environment. If the bacteria could aggregate by themselves, it will be easier to collect them. In the future, as a measure to remove bacteria from the environment, study of aggregation protein would be useful.
+
<p>
-
</dd>
+
We suggest that users should always check the safety risks and the rules of the parts,
-
 
+
devices, and systems. Also local authorities should be consulted. We should always keep in
 +
mind to take a look of the safety page related to the parts that you are going to use. To re-check
 +
safety issues with professional institutions. Our suggestions are very simple, but as long as you
 +
are a scientist and an iGEM members, they could not be forgotten.
 +
</p><p>Talking about benefits of our project, genetically engineered bacteria may accidentally spread
 +
out to the environment. If the bacteria could be made aggregate by themselves, it will be easier
 +
to collect them. In the future, as a measure to remove bacteria from the environment, study of
 +
aggregation protein would be useful.
 +
</p></dd>
</dl>
</dl>
 +
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Latest revision as of 13:58, 2 October 2012

Safety Questions

Risks to the safety and health of team members or others in the lab

All lab staff is trained according to safety manual provided by Hokkaido University. We took on ourselves to compile a shortlist of often used dangerous materials and safety procedures in our project.

Dangerous chemicals

  • Chloroform - corrosive and toxic: must be used in fume hood
  • Ethidium Bromide - intercalating agent: must be used with personal safety gear
  • Ethanol - flammable: must not be used near open flame or in large quantities/li>
  • Liquid Nitrogen - cryogenic container and cryogenic gloves must be used

Procedures and equipment

  • Agarose gel production - heating in sealed container (rupture risk), scalding hot and vicious during preparation (burn injury risk) - remove container lid before heating in microwave, use safety gear, wait for a few moments before removing from microwave
  • Benson burner - fire risk: DO NOT use flammable materials especially ethanol near open fire
  • Centrifuge - high velocity: balance appropriately, observe the machine till it reaches top velocity
  • Autoclave - high pressure: check the water level, DO NOT open when pressurized
  • UV radiation - damage to eyes and skin: use glove and UV box or UV shield

Non-pathogenic bacteria (policy requires treating as pathogenic, as precaution)

  • JM109
  • DH5alpha
Both of these are lab safe strains. As a precaution all materials coming in contact are sterilized before and after.

Reference Federal Register, (1986) Vol. V1: 88, 6952–16985

Safety equipment

  • Gloves
  • Coats
  • Goggles
  • UV Box
  • UV shield

Waste disposal and sterilization

All equipment and waste coming in contact with bacterial is sterilized by autoclave or bleach. All chemicals compounds were disposed according to requirements for their disposal. All table surface used for work were sterilized with 70% ethanol before and after a procedure

Chemical Usage

All chemical compounds were used according to their manuals and respective material safety data sheet

Genetic material

All genes used in this project come from non-pathogenic bacterial strains of E. coli or R. eutropha. Expressed proteins did not show any toxic effect to their host. Our biobricks do not have any foreseeable selective advantage if released to the environment. After consideration we could not find any usage pausing a security concern.

Risks to the safety and health of the general public if released by design or accident.

Also risks to environmental quality if released by design or accident. All material in the lab are kept safe and accessible only to the authorized lab members. All safety precaution were taken for waste disposal. Preventive actions as described previously were taken make sure the bacteria won’t be released into environment. In unlikely event of release bacteria is lab safe and doesn’t have selective advantage to propagate in nature. This bacteria and/or our biobrick are not designed for usage in public.

Risks to security through malicious misuse by individuals, groups or states. And do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

No biobricks made by us raise any safety issues. As described before all genes used in this project come from non-pathogenic bacterial strains of E. coli or R. eutropha. Expressed proteins did not show any toxic effect to their host. Our biobricks do not have any foreseeable selective advantage if released to the environment. After consideration we could not find any usage pausing a security concern. Bacterial strains are not pathogenic and lab safe.

Is there a local bio safety group, committee, or review board at your institution? If yes, what does your local bio safety group think about your project?

Project conducted at Hokkaido University involving recombination are overseen by officer of "the Safety Office of Genetic Recombination in Hokkaido University". Our lab is equipped appropriately for the manipulation and genetic recombination of bacterial cells. Team members are instructed according to the safety training manual. Permission to conduct our experiments was acquired.

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

We suggest that users should always check the safety risks and the rules of the parts, devices, and systems. Also local authorities should be consulted. We should always keep in mind to take a look of the safety page related to the parts that you are going to use. To re-check safety issues with professional institutions. Our suggestions are very simple, but as long as you are a scientist and an iGEM members, they could not be forgotten.

Talking about benefits of our project, genetically engineered bacteria may accidentally spread out to the environment. If the bacteria could be made aggregate by themselves, it will be easier to collect them. In the future, as a measure to remove bacteria from the environment, study of aggregation protein would be useful.


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