Team:Goettingen/week7-2

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Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
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<h2><b>V06_11 </b></h2><br>
<h2><b>V06_11 </b></h2><br>
<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
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+
<ul>
-
        <ul>
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<li>Experiment: <br>
-
                <li>Experiment: <br>
+
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
-
 
+
</ul>
-
        </ul>
+
<br>
 +
<b>V06_11_2 Chemical retransformation of the new <i>flhDC</i>-promoter constructs into <i>E. coli</i> (DH10B)</b><br>
 +
<ul>
 +
<li>Experiment:  <br>
 +
For the chemical retransformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed. The following constructs were transformed into <i>E. coli</i>:<br>
<br>
<br>
-
 
-
 
-
<b>V06_11_2 Chemical retransformartion of the new <i>flhDC</i>-promoter constructs into <i>E. coli</i> (DH10B)</b><br>
 
-
        <ul>
 
-
                <li>Experiment:  <br>
 
-
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into <i>E. coli</i>:<br>
 
20G - #1 <br>
20G - #1 <br>
20G - #2 <br>
20G - #2 <br>
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18K - #2 <br>
18K - #2 <br>
18K - #3 <br></li>
18K - #3 <br></li>
-
                <li>Observations & Results: <br>
+
<br>
-
The retransformation was successful since all plates showed numeours colonoes except the negative control.
+
<li>Observations & Results: <br>
-
    </ul>
+
The retransformation was successful since all plates showed numeours colonies except the negative control.
-
 
+
</ul>
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<br></td></tr>
<br></td></tr>
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</table>
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<li>Experiment: <br>
<li>Experiment: <br>
In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>
In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>
-
20E - #2 <br>
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<br>
-
20G - #1 <br>
+
- 20E - #2 <br>
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2G - #1 <br>
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- 20G - #1 <br>
-
20I - #2 <br>
+
- 2G - #1 <br>
-
18M - #2 <br>
+
- 20I - #2 <br>
-
18O - #2 <br>
+
- 18M - #2 <br>
-
18K - #1 <br>
+
- 18O - #2 <br>
-
18C - #2 <br>
+
- 18K - #1 <br>
 +
- 18C - #2 <br>
 +
<br>
<br></td></tr>
<br></td></tr>
</table>
</table>
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Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li></ul>
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li></ul>
<br>
<br>
-
<b>V06_13_2 Chemical transformartion of pBAD-<i>sfGFP</i> into <i>E. coli</i> (DH10B)</b><br>
+
<b>V06_13_2 Chemical transformation of pBAD-<i>sfGFP</i> into <i>E. coli</i> (DH10B)</b><br>
<ul>
<ul>
<li>Experiment:  <br>
<li>Experiment:  <br>
-
For the chemical transformation the standard protocol was followed. <br>
+
For the chemical transformation the standard <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> was followed. <br>
<li>Observations & Results: <br>
<li>Observations & Results: <br>
-
The transformation was successful since all plates showed numeours colonoes except the negative control.</li>
+
The transformation was successful since all plates showed numeours colonies except the negative control.</li>
</ul><br>
</ul><br>
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<br></td></tr>
</table>
</table>
 +
<br>
<br>
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<body id="top">
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
-
<a href="#top">&uarr; Return to top</a>
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<br>
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<b>Important pages</b>:<br>
 
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<a href="https://2012.igem.org/Team:Goettingen">Home</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
 
-
<a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
 
-
<a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
 
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<a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
 
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Latest revision as of 17:17, 22 September 2012

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#2 Speed Improvement - 7th week

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V06_11


V06_11_1 Miniprep of the new flhDC-promoter constructs
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformation of the new flhDC-promoter constructs into E. coli (DH10B)
  • Experiment:
    For the chemical retransformation the standard protocol was followed. The following constructs were transformed into E. coli:

    20G - #1
    20G - #2
    2G - #1
    2G - #2
    2G - #3
    20I - #2
    20I - #3
    18O - #2
    18O - #3
    18K - #1
    18K - #2
    18K - #3

  • Observations & Results:
    The retransformation was successful since all plates showed numeours colonies except the negative control.


V06_12


Preparation of over night cultures
  • Experiment:
    In order to gain further plasmid material over night cultures of one colonie of all eight flhDC-promoter constructs were prepared for subsequent plasmid isolation.

    - 20E - #2
    - 20G - #1
    - 2G - #1
    - 20I - #2
    - 18M - #2
    - 18O - #2
    - 18K - #1
    - 18C - #2



V06_13


V06_13_1 Miniprep of the new flhDC-promoter constructs
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_13_2 Chemical transformation of pBAD-sfGFP into E. coli (DH10B)
  • Experiment:
    For the chemical transformation the standard protocol was followed.
  • Observations & Results:
    The transformation was successful since all plates showed numeours colonies except the negative control.


V06_14


Preparation of over night cultures
  • Experiment:
    Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.


V06_15


Miniprep of pBAD-sfGFP
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.


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