Team:Bielefeld-Germany/Labjournal/week4

From 2012.igem.org

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Latest revision as of 21:12, 25 September 2012

Week 4 (05/21 - 05/27/12)

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

Navigate our Wiki

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 {{Team:Bielefeld/Head}}
-=Labjournal=
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1 Week1]
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-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2 Week2]
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-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3 Week3]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4 Week4]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5 Week5]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 Week6]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7 Week7]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8 Week8]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9 Week9]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10 Week10]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11 Week11]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12 Week12]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13 Week13]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19 Week19]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20 Week20]
-[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21 Week21]
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+#bodyContent{
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-==Week 4 (05/21 - 05/27/12)==
+</style>
-''' weekly seminar:'''
+<!-- navigator -->
-* Lab service: Isabel
+<div id="nav" class="tabs">
-* We try to establish a collaboration with the iGEM team from [https://2012.igem.org/Team:SDU-Denmark SDU-Denmark]
+ <div class="scroller">
-* Got our distribution kits
+	<ul style="list-style-type:none">
-* First successful cloning and cultivations
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li>
-* Who wants to be a summer school teacher?
+                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li>
-* We will not travel to the ACHEMA because only local teams are invited
+                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li>
-* Do we want to participate in the Biolympics? (It's a sports party with fun organized by the [http://bts-ev.de/ bts])
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li>
+		<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li>
+</ul>
-===Monday May 21st===
+ </div>
-* '''Team Modeling''': Our aims for modeling:
+</div>
+</html>
-** model the expression of the laccases in the organisms.
+<div style="text-align:justify;">
-** model the aktivity of our enzyms.
+==Week 4 (05/21 - 05/27/12)==
-** model the interesing parts of a clarification plant (the part witch are interesing for our cleaner.
-* '''Team Bacterial Laccases''': We did some restriction digests to clone our  laccase PCR products from ''Xanthomonas campestris'' and ''E. coli'' in pSB1C3 backbone.
-===Tuesday May 22nd===
-'''Team Bacterial Laccases''':
-We picked colonies from transformed BBa_J04450 and plated them on new LB plates with chloramphenicol.
-===Wednesday May 23rd===
-*''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells.  For setup see [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th here]. Got intense red fluorescing colonies but no green fluorescing colonies. Made a backup of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]. Asking for other plasmids containing GFP at the working groups of our University.
-*'''Team Bacterial Laccases''':
-** After there were no colonies on our pSB1C3 + CopA (''Xanthomonas campestris'') plate we did the transformation with the same ligation preparation again. For the other ligation with pSB1C3 + Cueo (''E. coli'') we started colony PCRSs to find postive colonies. Sadly the colony PCR showed no product.
-** Purification of the ''T. thermo'' laccase PCR product out of agarose gel.
-===Thursday May 24th===
-*'''Team Student Academy:''' Made a liquid culture of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] at 30°C. There was no fluorescence. Furthermore [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] was transformed. Also got intense red fluorescing colonies.
-===Friday May 25th===
-'''Team Bacterial Laccases''':
-* We had to do the PCR on ''T. thermo'' laccase again, because before the amount of purified PCR product was very low.
-===Saturday May 26th===
+{{Team:Bielefeld/Sponsoren}}
-===Sunday May 27th===