Team:Goettingen/Test

From 2012.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 1: Line 1:
-
{{Team:Goettingen/Header}}
+
{{GoettingenHeader}}
 +
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
 +
<meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
 +
<link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
 +
<link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
 +
<link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&amp;action=creativecommons" rel="meta">
 +
<link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=rss">
 +
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&amp;feed=atom">
 +
</head>
 +
<body class="mediawiki ns-0 ltr page-Team_Goettingen">
<table>
<table>
-
                                <!-- Text body -->
 
<tbody><tr valign="top" align="left">
<tbody><tr valign="top" align="left">
Line 12: Line 22:
<br>
<br>
-
<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div>
+
<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle"><a href="javascript:toggleToc()" class="internal" id="togglelink"></a></span></div>
<ul>
<ul>
<li class="toclevel-1"><a href="#Welcome"><span class="tocnumber">1</span> <span class="toctext">Welcome to iGEM G&ouml;ttingen</span></a></li>
<li class="toclevel-1"><a href="#Welcome"><span class="tocnumber">1</span> <span class="toctext">Welcome to iGEM G&ouml;ttingen</span></a></li>
Line 20: Line 30:
</td></tr></tbody></table>
</td></tr></tbody></table>
<br>
<br>
-
 
Line 26: Line 35:
<p align="justify" style="line-height:1.6em">
<p align="justify" style="line-height:1.6em">
-
iGEM is an international competition hosted by the MIT in Boston, USA, for undergraduate students
+
In November 2011, most of us heard about iGEM competition for the first time.
-
of disciplines related to molecular biology. iGEM stands for International Genetically Engineered
+
Erik, inspired by his former colleagues´ enthusiastic reports, was looking for like-minded people at his new university that
-
Machine competition. On the one hand, it targets to combine aspects of education and social
+
were willing to organize a whole project in the field of Synthetic Biology. He described the idea of the contest to anybody willing to listen. It did not take
-
collaboration among undergraduate students, on the other hand, it provides a library for
+
long to find five other interested students. Thus, the so called organization task force of the first iGEM team of the University of Göttingen was formed.
-
standardized and interchangeable parts which can be used in living systems, particularly
+
<br><br>
-
in model organisms like <i>E. coli</i>. Student groups from all over the world will receive a
+
Luckily, the iGEM competition was already well known among the University´s professors and the team found his first supporters: Prof. Heinz
-
requested kit of biological parts, also called "biobricks", and work over the summer on
+
Neumann, head of a work group specializing in Synthetic Biology, and Prof. Jörg Stülke from the department of General and Applied Microbiology.
-
an individual research project. Every year, the "Registry of Standard Biological Parts"
+
The next task was to find a project that was interesting as well as realizable. After a lot of back and forth due to extensive discussions, Prof.
-
is upgraded with further biobricks by the participating iGEM teams. These biobricks will
+
Neumann presented the perfect project: "Homing Coli"!
-
be accessible to the iGEM community in the following years in order to use these parts for
+
<br><br>
-
their own projects.  <br>
+
After many drawbacks, like finding a suitable laboratory to realize the project, the search for additional team members began. The new
-
<br>
+
fellows were quickly found, which was the story starting point of the first iGEM team of the University of Göttingen… Below you can see our timeline.<br><br>
-
 
+
-
The iGEM competition started in 2003 as a course for students of the MIT only. 2011,
+
-
already 165 universities from all over the world competed against each other. This year,
+
-
it is the first participation of the University of G&ouml;ttingen, Germany. The iGEM competition
+
-
is a great opportunity for students to gain and improve a multitude of skills that are
+
-
necessary during a carrier as a molecular biologist. Such skills would be planning and
+
-
organizing a project, including fund raising and team recruitment, coordination of lab
+
-
work, designing experiments and working together as a team. Next to intensive lab work
+
-
during the summer, also literature search and well-structured documentation of the
+
-
experiments is crucial. Moreover, safety for humans and environment is key.<br>
+
 +
<a href="https://static.igem.org/mediawiki/2012/0/0d/Timeline.png"><img width="650px" src="https://static.igem.org/mediawiki/2012/0/0d/Timeline.png"></a><br>
 +
The timeline of the iGEM team Göttingen 2012. Click on the picture for full size.
 +
<br><br>
 +
If you are interested in the <a href="https://2012.igem.org/Team:Goettingen/iGEM">iGEM competition</a>, the BioBrick concept, and – most importantly – <a href="https://2012.igem.org/Team:Goettingen/Project">our project</a>, take a look around. Here, you will find everything
 +
you need to know about "Homing Coli" and you will have the opportunity to gather knowledge about chemotaxis in general!
 +
<br>
 +
Visit our <a href="https://2012.igem.org/Team:Goettingen/Team">team site</a> to learn more about us and our supporters and advisors!
 +
And, last but not least, do not forget to check out our mascot  <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">"Flash Coli"</a>! He always has some interesting stories to tell and needs YOUR help to fight the horrible phages!
 +
<br><br>
 +
Feel free to browse this page!<br><br>
</p>
</p>
-
 
<br>
<br>
 +
 +
 +
Line 74: Line 85:
beneficial fast phenotype can be combined with the ability of this bacterium to sense
beneficial fast phenotype can be combined with the ability of this bacterium to sense
specific compounds in their environments. Chemoreceptors enable it to move towards
specific compounds in their environments. Chemoreceptors enable it to move towards
-
or along gradients of such substances. We will use PCR-based site directed mutagenesis on the <i> E. coli</i> TAR receptor. The combination of speed and chemotaxis allows
+
or along gradients of such substances. We will use PCR-based site directed mutagenesis on the <i> E. coli</i> Tar receptor. The combination of speed and chemotaxis allows
us to identify <i>E. coli</i> strains, which can sense interesting compounds. Thereby, an
us to identify <i>E. coli</i> strains, which can sense interesting compounds. Thereby, an
easy method for the detection of pollutants, toxins or even tumors could be provided.<br><br>
easy method for the detection of pollutants, toxins or even tumors could be provided.<br><br>
Line 80: Line 91:
<table cellpadding="3"><tr><td>
<table cellpadding="3"><tr><td>
<img width="400 px" src="http://www.patrickreinke.de/igem/homingcoli.jpg"></td>
<img width="400 px" src="http://www.patrickreinke.de/igem/homingcoli.jpg"></td>
-
<td valign="top"; cellpadding="30"><font size="-1">  <a href="http://jb.asm.org/content/186/22/7529.full"> <p align="justify">Clive S. Barker <i> et al. </i> (2004). Increased Motility of <i> Escherichia coli </i> by Insertion Sequence Element
+
<td valign="top"; cellpadding="30"><font size="-2">  <a href="http://jb.asm.org/content/186/22/7529.full"> <p align="justify">Clive S. Barker <i> et al. </i> (2004). Increased Motility of <i> Escherichia coli </i> by Insertion Sequence Element
Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.</a> </font><br>
Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.</a> </font><br>
</td></tr>
</td></tr>
Line 87: Line 98:
<br>
<br>
 +
<font size="-1">
<h2><b><a name="Synthetic_Biology"></a>Synthetic Biology</b></h2>
<h2><b><a name="Synthetic_Biology"></a>Synthetic Biology</b></h2>
Line 108: Line 120:
<img src="http://www.patrickreinke.de/igem/synbio.jpg">
<img src="http://www.patrickreinke.de/igem/synbio.jpg">
<br>
<br>
-
<font size="-1">After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (<b>A</b>) can be subject to two basic types of synthetic manipulation. (<b>B</b>) Autonomous synthetic circuits, consisting of ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows) seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function (purple arrows) (<b>C</b>)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity. (<b>D</b>) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be able to design and construct (<b>E</b>) synthetic circuits that can functionality replace endogenous circuits or (<b>F</b>) fully autonomous circuits that operate independently of the cellular mileu.</font>
+
<font size="-2">After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (<b>A</b>)
-
 
+
can be subject to two basic types of synthetic manipulation. (<b>B</b>) Autonomous synthetic circuits, consisting of
-
 
+
ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows)
-
        <font size="-1">  <a href="http://www.its.caltech.edu/~haylab/research/SBReview2011.pdf">Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated
+
seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function
 +
(purple arrows) (<b>C</b>)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity.
 +
(<b>D</b>) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired
 +
endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be
 +
able to design and construct (<b>E</b>) synthetic circuits that can functionality replace endogenous circuits or (<b>F</b>)
 +
fully autonomous circuits that operate independently of the cellular mileu.
 +
<a href="http://www.its.caltech.edu/~haylab/research/SBReview2011.pdf">Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated
Gene Circuits. SCIENCE, Vol. 333: 1244-1248.</a> </font><br>
Gene Circuits. SCIENCE, Vol. 333: 1244-1248.</a> </font><br>
<br>
<br>
 +
<font size="-1">
Another very important and necessary feature of biological parts is orthogonality,
Another very important and necessary feature of biological parts is orthogonality,
which means in this context that independent devices can be combined unrestrictedly.
which means in this context that independent devices can be combined unrestrictedly.
Line 170: Line 189:
<hr><h2> <span><b>Flash Coli</b></span></h2>
<hr><h2> <span><b>Flash Coli</b></span></h2>
<!-- News cap end -->
<!-- News cap end -->
-
<embed src="http://www.patrickreinke.de/igem/motionflashcoli.swf" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" wmode="transparent" height="180" width="240">
+
<embed src="http://www.patrickreinke.de/igem/motionflashcoli.swf" type="application/x-shockwave-flash"
 +
pluginspage="http://www.macromedia.com/go/getflashplayer" wmode="transparent" height="180" width="240">
<font face="Verdana" size="-1">To explore our project with Flash Coli click
<font face="Verdana" size="-1">To explore our project with Flash Coli click
<a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here</a>.
<a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here</a>.
Line 190: Line 210:
<br>
<br>
           <!-- News 17 -->
           <!-- News 17 -->
-
<p align="left"><i>07 Septembre 2012,</i>  <b>We organized the "Legoland for Scientists" symposium, at the University of Goettingen. You can read everything <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion">here</a></b>
+
<p align="left"><i>07 September 2012,</i>  <b>We organized the "Legoland for Scientists" symposium at the University of Göttingen.
 +
You can read everything about it <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion">here.</a></b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#17">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#17">To the news</a><br>
Line 196: Line 217:
           <!-- News 16 -->
           <!-- News 16 -->
-
<p align="left"><i>30 August 2012,</i>  <b>The first version of 'Flash Coli - the game' is online now. click <a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here</a></b>
+
<p align="left"><i>30 August 2012,</i>  <b>The first version of "Flash Coli - the game" is now online. Click
 +
<a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli">here.</a></b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
Line 202: Line 224:
           <!-- News 15 -->
           <!-- News 15 -->
-
<p align="left"><i>25 August 2012,</i>  <b>Today is the "synthetic biology" day in Germany!</b>
+
<p align="left"><i>25 August 2012,</i>  <b>Today is the "Synthetic Biology" day in Germany!</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#15">To the news</a><br>
Line 208: Line 230:
           <!-- News 14 -->
           <!-- News 14 -->
-
<p align="left"><i>15 August 2012,</i>  <b>In our weekly seminar Anna presented an example for synthetic Biology</b>
+
<p align="left"><i>15 August 2012,</i>  <b>In our weekly seminar Anna presented an example for Synthetic Biology.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#14">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#14">To the news</a><br>
Line 216: Line 238:
           <!-- News 13 -->
           <!-- News 13 -->
-
<p align="left"><i>08 August 2012,</i>  <b>Ali and Sandra conducted their presentations in our weekly seminar</b>
+
<p align="left"><i>08 August 2012,</i>  <b> Survey: Ready to be asked? </b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#13">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#13">To the news</a><br>
         <!-- News 13 end--><br><hr>
         <!-- News 13 end--><br><hr>
 +
 +
 +
 +
        <!-- News 12.75 -->
 +
<p align="left"><i>01 August 2012,</i>  <b>Ali and Sandra conducted their presentations in our weekly seminar.</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#12.75">To the news</a><br>
 +
        <!-- News 12.75 end--><br><hr>
 +
 +
 +
 +
        <!-- News 12.5 -->
 +
<p align="left"><i>19 August 2012,</i>  <b>Human practice task force established!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#12.5">To the news</a><br>
 +
        <!-- News 12.5 end--><br><hr>
           <!-- News 12 -->
           <!-- News 12 -->
-
<p align="left"><i>12 July 2012,</i>  <b>In today’s weekly meeting Christian told us about the European teacher meet-up in Paris</b>
+
<p align="left"><i>12 July 2012,</i>  <b>In today’s weekly meeting Christian told us about the European teacher meet-up in Paris.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#12">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#12">To the news</a><br>
         <!-- News 12 end--><br><hr>
         <!-- News 12 end--><br><hr>
 +
 +
 +
 +
      <!-- News 11.5 -->
 +
<p align="left"><i>04 July 2012,</i>  <b>Let there be light!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#11.5">To the news</a><br>
 +
        <!-- News 11.5 end--><br><hr>
 +
           <!-- News 11 -->
           <!-- News 11 -->
-
<p align="left"><i>28 June 2012,</i>  <b>Anna, Corinna and Erik participated in the third annual congress for strategy processes ”Biotechnology 2020+”</b>
+
<p align="left"><i>28 June 2012,</i>  <b>Anna, Corinna and Erik participated in the third annual congress for strategy processes "Biotechnology 2020+".</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#11">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#11">To the news</a><br>
         <!-- News 11 end--><br><hr>
         <!-- News 11 end--><br><hr>
 +
 +
 +
 +
      <!-- News 10.5 -->
 +
<p align="left"><i>26 June 2012,</i>  <b><i>E. coli</i> strain spurred!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#10.5">To the news</a><br>
 +
        <!-- News 10.5 end--><br><hr>
           <!-- News 10 -->
           <!-- News 10 -->
-
<p align="left"><i>21 June 2012,</i>  <b>Our first poster is designed</b>
+
<p align="left"><i>21 June 2012,</i>  <b>Our first poster is designed!</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#10">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#10">To the news</a><br>
         <!-- News 10 end--><br><hr>
         <!-- News 10 end--><br><hr>
 +
 +
 +
 +
        <!-- News 9.5 -->
 +
<p align="left"><i>13 June 2012,</i>  <b>Task: Find a human practice project!</b>
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#9.5">To the news</a><br>
 +
        <!-- News 9.5 end--><br><hr>
           <!-- News 9 -->
           <!-- News 9 -->
-
<p align="left"><i>06 June 2012,</i>  <b>Thomas Maschner from LMU Munich came to visit</b>
+
<p align="left"><i>06 June 2012,</i>  <b>Thomas Maschner, advisor from the iGEM team of the LMU Munich, came to visit us.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#9">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#9">To the news</a><br>
         <!-- News 9 end--><br><hr>
         <!-- News 9 end--><br><hr>
 +
 +
 +
 +
        <!-- News 8.5 -->
 +
<p align="left"><i>01 June 2012,</i>  <b>Discussion of the first lab results</b> 
 +
</p>
 +
<a href="https://2012.igem.org/Team:Goettingen/News#8.5">To the news</a><br>
 +
        <!-- News 8.5 end--><br><hr>
           <!-- News 8 -->
           <!-- News 8 -->
-
<p align="left"><i>15 May 2012,</i>  <b>The first picture of our complete Team</b>
+
<p align="left"><i>15 May 2012,</i>  <b>The first picture of our complete team.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#8">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#8">To the news</a><br>
Line 264: Line 335:
           <!-- News 7 -->
           <!-- News 7 -->
-
<p align="left"><i>11 May 2012,</i>  <b>iGEM Göttingen is in the local Newspaper</b>
+
<p align="left"><i>11 May 2012,</i>  <b>iGEM Göttingen is in the local newspaper.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#7">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#7">To the news</a><br>
Line 272: Line 343:
         <!-- News 6 --> <br>
         <!-- News 6 --> <br>
-
<p align="left"><i>04 May 2012,</i>  <b><a href="http://www.kws.com/">KWS SAAT AG</a> is now Head Sponsor</b>
+
<p align="left"><i>04 May 2012,</i>  <b><a href="http://www.kws.com/">KWS SAAT AG</a> is now Head Sponsor.</b>
</p>
</p>
<p align="left">
<p align="left">
Line 282: Line 353:
         <!-- News 5 -->
         <!-- News 5 -->
-
<p align="left"><i>02 May 2012,</i>  <b>Today Anna presented our iGEM Logo</b>
+
<p align="left"><i>02 May 2012,</i>  <b>Today Anna presented our iGEM logo.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#5">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#5">To the news</a><br>
Line 290: Line 361:
         <!-- News 4 -->
         <!-- News 4 -->
-
<p align="left"><i>30 April 2012,</i>  <b>We inspect the Laboratory at the Max Planck Campus</b>
+
<p align="left"><i>30 April 2012,</i>  <b>We inspect the laboratory at the Max Planck campus.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#4">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#4">To the news</a><br>
Line 298: Line 369:
         <!-- News 3 -->
         <!-- News 3 -->
-
<p align="left"><i>26 April 2012,</i>  <b>Weekly meeting: PCR-Lecture from Heinz</b>
+
<p align="left"><i>26 April 2012,</i>  <b>Weekly meeting: PCR-Lecture from Heinz.</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#3">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#3">To the news</a><br>
Line 306: Line 377:
         <!-- News 2 -->
         <!-- News 2 -->
-
<p align="left"><i>19 April 2012,</i>  <b>The iGEM Team Göttingen 2012 is now complete!</b>
+
<p align="left"><i>19 April 2012,</i>  <b>The iGEM team Göttingen 2012 is now complete!</b>
</p>
</p>
<a href="https://2012.igem.org/Team:Goettingen/News#2">To the news</a><br>
<a href="https://2012.igem.org/Team:Goettingen/News#2">To the news</a><br>
Line 314: Line 385:
         <!-- News 1 -->  <br>
         <!-- News 1 -->  <br>
-
</p><p><i>04 April 2012,</i>    <b> <a href="http://www.sartorius.com/">Sartorius Stedim Biotech GmbH</a> is now Premium Sponsor</b>
+
</p><p><i>04 April 2012,</i>    <b> <a href="http://www.sartorius.com/">Sartorius Stedim Biotech GmbH</a> is now Premium Sponsor.</b>
</p>
</p>
<p>
<p>
Line 323: Line 394:
<br>
<br>
</font>
</font>
-
 
-
 
-
 
-
 
-
 
-
                                <!-- News body Ende -->
 
-
 
</font></td></tr></tbody>
</font></td></tr></tbody>
-
 
</table></div>
</table></div>
-
 
</td></tr>
</td></tr>
-
 
+
</body>
-
{{Team:Goettingen/Footer}}
+
</html>
 +
{{GoettingenFooter}}

Latest revision as of 14:03, 20 September 2012

Deutsch  / English 


Language: English, Deutsch

Contents


Welcome to iGEM Göttingen

In November 2011, most of us heard about iGEM competition for the first time. Erik, inspired by his former colleagues´ enthusiastic reports, was looking for like-minded people at his new university that were willing to organize a whole project in the field of Synthetic Biology. He described the idea of the contest to anybody willing to listen. It did not take long to find five other interested students. Thus, the so called organization task force of the first iGEM team of the University of Göttingen was formed.

Luckily, the iGEM competition was already well known among the University´s professors and the team found his first supporters: Prof. Heinz Neumann, head of a work group specializing in Synthetic Biology, and Prof. Jörg Stülke from the department of General and Applied Microbiology. The next task was to find a project that was interesting as well as realizable. After a lot of back and forth due to extensive discussions, Prof. Neumann presented the perfect project: "Homing Coli"!

After many drawbacks, like finding a suitable laboratory to realize the project, the search for additional team members began. The new fellows were quickly found, which was the story starting point of the first iGEM team of the University of Göttingen… Below you can see our timeline.


The timeline of the iGEM team Göttingen 2012. Click on the picture for full size.

If you are interested in the iGEM competition, the BioBrick concept, and – most importantly – our project, take a look around. Here, you will find everything you need to know about "Homing Coli" and you will have the opportunity to gather knowledge about chemotaxis in general!
Visit our team site to learn more about us and our supporters and advisors! And, last but not least, do not forget to check out our mascot "Flash Coli"! He always has some interesting stories to tell and needs YOUR help to fight the horrible phages!

Feel free to browse this page!


Homing Coli

Escherichia coli is a commonly used bacterial model organism. It has lots of beneficial traits, e.g. a short generation time and it can be easily manipulated. Most E. coli strains that are used in laboratories do not exhibit high motility. The crucial element for motility is the flagellum, which is rotated by a molecular motor within the cell wall. Consequently, these are reduced in cultivated E. coli strains.

Our goal is to create an E. coli strain with increased swimming motility on special agar plates. Therefore, we will overexpress regulators and parts of the E. coli flagellum in cultivated strains in order to enhance bacterial swimming ability. The fastest E. coli strains will be selected and further improved. At the same time we will then be able to create an effective motility-selection method.

Now, you are probably wondering what the advantage of a fast E. coli might be. The beneficial fast phenotype can be combined with the ability of this bacterium to sense specific compounds in their environments. Chemoreceptors enable it to move towards or along gradients of such substances. We will use PCR-based site directed mutagenesis on the E. coli Tar receptor. The combination of speed and chemotaxis allows us to identify E. coli strains, which can sense interesting compounds. Thereby, an easy method for the detection of pollutants, toxins or even tumors could be provided.

Clive S. Barker et al. (2004). Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon. Journal of Bacteriology, Vol. 186: 7529-7537.


Synthetic Biology

Synthetic biology is an interdisciplinary scientific area that has recently developed. It links various fields of science like biology, chemistry, physics, molecular genetics, informatics and engineering.

Due to this combination the relation of biological design and function can be investigated from an entirely new perspective. The previous approach was limited to the examination of a structure and its function and the attempt to explain how they correlate. Recently, the reverse strategy is applied. Biological parts are specifically designed and constructed according to a desired function. These parts are characterized by a standardized modular design that facilitates their handling. The subsequent introduction of the synthetic constructs to living cells can either cause the replacement of original cellular components or result in additional elements that act cooperatively or more or less autonomously (see fig. 1).


After Nandagopaland and Elowitz (2011). A continuum of synthetic biology. Wild-type cells (A) can be subject to two basic types of synthetic manipulation. (B) Autonomous synthetic circuits, consisting of ectopic components, may be introduced into the cell. Such circuits process inputs and implement functions (red arrows) seperate from the endogenous circuitry (black). However, unknown interactions with the host cell may affect their function (purple arrows) (C)An alternative is to rewire (red lines) the endogenous circuits themselves to have new connectivity. (D) Extending this line of synthetic manipulation, synthetic circuits could be integrated into appropriately rewired endogenous circuitry to act as sensors and to implent additional functionality. Ultimate goals of this program are to be able to design and construct (E) synthetic circuits that can functionality replace endogenous circuits or (F) fully autonomous circuits that operate independently of the cellular mileu. Nagarajan Nandagopal and Michael B. Elowitz. (2011). Synthetic Biology: Integrated Gene Circuits. SCIENCE, Vol. 333: 1244-1248.

Another very important and necessary feature of biological parts is orthogonality, which means in this context that independent devices can be combined unrestrictedly. This principle derives from the area of engineering and aims to alter subsystems, without impeding others.

This way cells can be modified as requested, resulting in a predictable behaviour. Among others, this technique can be beneficial for the effective production of certain substances, like biofuels or antibiotics.



↑ Return to top

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

Flash Coli

To explore our project with Flash Coli click here.


News


07 September 2012, We organized the "Legoland for Scientists" symposium at the University of Göttingen. You can read everything about it here.

To the news

30 August 2012, The first version of "Flash Coli - the game" is now online. Click here.

To the news

25 August 2012, Today is the "Synthetic Biology" day in Germany!

To the news

15 August 2012, In our weekly seminar Anna presented an example for Synthetic Biology.

To the news

08 August 2012, Survey: Ready to be asked?

To the news

01 August 2012, Ali and Sandra conducted their presentations in our weekly seminar.

To the news

19 August 2012, Human practice task force established!

To the news

12 July 2012, In today’s weekly meeting Christian told us about the European teacher meet-up in Paris.

To the news

04 July 2012, Let there be light!

To the news

28 June 2012, Anna, Corinna and Erik participated in the third annual congress for strategy processes "Biotechnology 2020+".

To the news

26 June 2012, E. coli strain spurred!

To the news

21 June 2012, Our first poster is designed!

To the news

13 June 2012, Task: Find a human practice project!

To the news

06 June 2012, Thomas Maschner, advisor from the iGEM team of the LMU Munich, came to visit us.

To the news

01 June 2012, Discussion of the first lab results

To the news

15 May 2012, The first picture of our complete team.

To the news

11 May 2012, iGEM Göttingen is in the local newspaper.

To the news


04 May 2012, KWS SAAT AG is now Head Sponsor.

To the news

02 May 2012, Today Anna presented our iGEM logo.

To the news

30 April 2012, We inspect the laboratory at the Max Planck campus.

To the news

26 April 2012, Weekly meeting: PCR-Lecture from Heinz.

To the news

19 April 2012, The iGEM team Göttingen 2012 is now complete!

To the news


04 April 2012, Sartorius Stedim Biotech GmbH is now Premium Sponsor.

To the news
↑ Back to top!

Retrieved from "http://2012.igem.org/Team:Goettingen/Test"