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- | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
| + | {{GoettingenHeader|deu=Team:Goettingen/week19-1|eng=Team:Goettingen/week19-1}} |
- | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
| + | <html> |
- | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
| + | <table> |
- | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
| + | |
- | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
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- | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
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- | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss">
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- | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
| + | |
| | | |
| | | |
- | <body class="mediawiki ns-0 ltr page-Team_Goettingen">
| |
| | | |
| | | |
| | | |
| | | |
- | <!-- start content -->
| |
| | | |
| | | |
| + | <!-- Text body --> |
| + | <tbody> |
| + | <tr valign="top" align="left"> |
| + | <td style="padding: 0pt 20px 0pt 0pt;" width="900px"> |
| + | <font face="Verdana" size="-1"> |
| + | <h2><b><a name="week3#2">#1 Selection / Swimming - 19th Week</a></b></h2> |
| + | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> |
| + | <br> |
| + | <p align="justify" style="line-height:1.6em"> |
| + | </p> |
| | | |
- | <style> | + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
- | h1.firstHeading { display: none; }
| + | <tr bordercolor="black" valign="top"> |
| + | <td width="900" bordercolor="black" valign="top"> |
| + | <h2><b>V09_03 </b></h2><br> |
| | | |
- | p {text-align: justify;}
| + | <b>V09_03_1: Third round of selection of the second approach of the <i>tar</i> mutagenesis library</b> |
| + | <ul> |
| + | <li>Experiment: <br>View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
| + | <li>Observations: <br> |
| + | Swimming could be observed, continuation: V09_04_5 |
| + | </ul> |
| + | <br> |
| | | |
- | a:link { color: #004080; text-decoration: none}
| + | <b>V09_03_2: First round of selection of the third approach of the <i>tar</i> mutagenesis library</b> |
- | a:visited { color:##003090; text-decoration: none} | + | <ul> |
- | a:hover { color:#f29400; text-decoration: none}
| + | <li>Experiment: <br>View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
- | a:active { color:#f29400; text-decoration: none}
| + | <li>Observations: <br> |
| + | Observation: Swimming on every plate, continuation: V09_04_4 |
| + | </ul> |
| + | <br> |
| | | |
- | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
| |
| | | |
- | table#team_members { text-align: justify; border: 0; }
| + | <b>V09_03_3: Electron microscopy</b> |
- | table#team_members h2, table#team_members h3 { clear: both; }
| + | <ul> |
| + | <li>Experiment: <br>Experiment: Electron microcopy with the strains BL21 + <i>rfp, +flhDC, +flicC</i>and MG1655</a>.</li> |
| + | <li>Observations: <br> |
| + | Observation: view focus group 2 |
| + | </ul> |
| + | <br> |
| + | <br></td></tr> |
| + | </table> |
| | | |
| | | |
- | /*-----------------------------------------------------------------------------------------------*/ | + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
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| + | <tr bordercolor="black" valign="top"> |
- | display: none; /* Hides all drop-down menus. */
| + | <td width="900" bordercolor="black" valign="top"> |
| + | <h2><b>V09_04 </b></h2><br> |
| + | <b>V09_04_1: Observation of the growth of the strains used for EM, BL21 + <i>rfp, +flhDC, +flicC</i>and MG1655</b><br> |
| + | <ul> |
| + | <li>Experiment: <br>In order to gain a higher cell density which is necessary of EM, the growth of the cells in different incubation conditions was observed. The cells were grown in 5 mL LB-broth.</li> |
| + | <li>Observations: <br> |
| + | The bacteria grow very slowly and the motility is not that high. |
| + | </ul> |
| + | <br> |
| | | |
- | }
| + | <b>V09_04_2: Quantification of the effect of pSB1C3_<i>tar</i>_QC with different promotors in the strain BL21</b><br> |
- | /* | + | <ul> |
- | li:hover works in IE7 and FF2.
| + | <li>Experiment: <br>A standard swimming/chemotaxis assay was conducted with BL21 strains containing <i>tar</i> rescue plasmids with different promotors respectively. View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
- | a:hover works in IE6 and FF2.
| + | <li>Observations: <br> |
- | a:hover breaks li:hover in FF2.
| + | 09_05: no swimming was observed |
- | */
| + | </ul> |
- | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
| + | <br> |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| + | |
- | display: none; /* Hides all side menus. */
| + | |
- | }
| + | |
- | /*------------------------------------------------------------------------------------- Menu Bar */ | + | |
- | div.MenuBar {
| + | |
- | font: Verdana;
| + | |
- | height: 30px;
| + | |
- | width: 910px;
| + | |
- | /*width: 100%*/
| + | |
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| + | |
- | border-left: 0;
| + | |
- | padding: 0;
| + | |
- | background: #00446b;
| + | |
| | | |
- | }
| + | <b>V09_04_3: Separation assay</b><br> |
- | div.MenuBar ul {
| + | <ul> |
- | font: Verdana;
| + | <li>Experiment: <br>Experiment: The strains Δ<i>tar</i> J61002_rfp and Δ<i>tar</i> _tar_QC_18C were dropped in the same ratio on 0.3% tryptone swimming agar plates.</li> |
- | text-align: center;
| + | <li>Observations: <br> |
- | list-style-type: none;
| + | 09_05: only little swimming and no chemotaxis could be observed. |
- | margin: 0 auto;
| + | </ul> |
- | border: 0;
| + | <br> |
- | padding: 0;
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| + | |
- | div.MenuBar ul li {
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- | font: Verdana;
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- | font-size: 1.3em;
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- | float: left;
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| + | |
- | }
| + | |
| | | |
- | div.MenuBar ul li a {
| |
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| |
- | text-decoration: none;
| |
- | }
| |
| | | |
- | /*------------------------------------------------------------------------------ Drop-Down Menus */ | + | <b>V09_04_4: Second round of selection of the third approach of the <i>tar</i> mutagenesis library</b><br> |
- | div.MenuBar ul li:hover ul.DropDownMenu,
| + | <ul> |
- | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | <li>Experiment: <br>View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
- | display: block;
| + | <li>Observations: <br> |
- | width: 10em; /* Drop-down menu width.
| + | Continuation in 09_05_1 |
- | Use MenuTailor.css to customize. */
| + | </ul> |
- | height: 1em;
| + | <br> |
- | padding: 1px; /* Sets the drop-down menu "effective border" width. */
| + | |
- | position: absolute;
| + | |
- | top: 28px; /* Places the drop-down menu under the menu bar.
| + | |
- | Keep it sync with the menu bar height. */
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| + | |
- | background-color: #A3C0E0; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
| | | |
- | }
| + | <b>V09_04_5: “Plating of the clones on LB agar containing CM” of the second approach of the <i>tar</i> mutagenesis library </b><br> |
- | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | <ul> |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | <li>Experiment: <br>View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
- | width: 10em; /* Keep it sync with the drop-down menu width.
| + | <li>Observations: <br> |
- | Use MenuTailor.css to customize. */
| + | Growth was observed on ever plates, continuation: V09_06_3 |
- | height: 1em;
| + | </ul> |
- | padding-left: 0;
| + | <br> |
- | padding-right: 0;
| + | |
- | background-color: #A3C0E0; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | ul.DropDownMenu li a span {
| + | |
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| + | |
- | }
| + | |
- | ul.DropDownMenu li a span span { | + | |
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| + | |
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| + | |
- | }
| + | |
- | /*----------------------------------------------------------------------------------- Side Menus */
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
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| + | |
- | display: block;
| + | |
- | width: 11em; /* Side menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
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| + | |
- | top: -1px; /* Aligns the side menu to its drop-down menu item.
| + | |
- | Keep it sync with the side menu "effective border" width. */
| + | |
- | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
- | width: 11em; /* Keep it sync with the side menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | font: 12px Verdana; /* Required for IE55. */
| + | |
- | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | }
| + | |
- | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| + | |
- | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| + | |
- | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| + | |
- | text-align: left;
| + | |
- | font: 12px Verdana; /* Required for IE55. */
| + | |
- | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width.
| + | |
- | Use MenuTailor.css to customize. */
| + | |
- | }
| + | |
- | /*----------------------------------------------------------------------------- Browser Specific */
| + | |
- | * html div.MenuBar ul li a {
| + | |
- | float: left; /* Required for IE55 and IE6.
| + | |
- | Breaks O9.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | * html ul.DropDownMenu li a:hover {
| + | |
- | cursor: hand; /* Required for IE55.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | ul.DropDownMenu li a:hover {
| + | |
- | cursor: pointer; /* Required for IE6 and IE7.
| + | |
- | Hidding it (* html) from non-IE browsers breaks IE7!
| + | |
- | }
| + | |
- | * html div.MenuBar a:hover {
| + | |
- | text-decoration: none; /* Required for IE55 and IE6.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | * html div.MenuBar ul li table,
| + | |
- | * html div.MenuBar ul li table td {
| + | |
- | border: 0; /* Required for IE55 and IE6.
| + | |
- | Hidden (* html) from non-IE browsers. */
| + | |
- | }
| + | |
- | /*------------------------------------------------------------------------------- Default Colors */
| + | |
- | div.MenuBar {
| + | |
- | background-color: Menu;
| + | |
- | border-bottom: 1px solid ButtonShadow;
| + | |
- | }
| + | |
- | div.MenuBar a {
| + | |
- | background-color: Menu; /* Top-level unselected items. */
| + | |
- | color: MenuText;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover a,
| + | |
- | div.MenuBar ul li a:hover {
| + | |
- | color: #A3C0E0;
| + | |
- | background-color: Highlight; /* Top-level selected item. */
| + | |
- | color: HighlightText;
| + | |
- | }
| + | |
- | /*...............................................................................................*/
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
- | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
- | background-color: Menu; Drop-down menu unselected items.
| + | |
- | Sets the drop-down menu "effective background" color. */
| + | |
- | color: MenuText;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| + | |
- | background-color: Highlight; /* Drop-down menu selected item. */
| + | |
- | color: HighlightText;
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| + | |
- | /*...............................................................................................*/
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
- | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
- | background-color: Menu; /* Side menu unselected items.
| + | |
- | Sets the side menu "effective background" color. */
| + | |
- | color: MenuText;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
- | background-color: Highlight; /* Side menu selected item. */
| + | |
- | color: HighlightText;
| + | |
- | }
| + | |
- | /*-----------------------------------------------------------------------------------------------*/
| + | |
| | | |
- | /*-------------------------------------------------------------------------------------- General */ | + | <br></td></tr> |
- | body {
| + | </table> |
- | background: white;
| + | |
- | color: black;
| + | |
- | margin: 0;
| + | |
- | border: 0;
| + | |
- | padding: 0;
| + | |
- | }
| + | |
| | | |
| | | |
- | div.MenuBar {
| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
- | font: 13px arial, helvetica, sans-serif;
| + | <tr bordercolor="black" valign="top"> |
- | }
| + | <td width="900" bordercolor="black" valign="top"> |
- | div.MenuBar ul {
| + | <h2><b>V09_05 </b></h2><br> |
- | font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
| + | <b>V09_05_1: Third round of selection of the 3rd approach of the <i>tar</i> mutagenesis library </b><br> |
- | }
| + | <ul> |
- | /*--------------------------------------------------------------------------------------- Colors */
| + | <li>Experiment: <br>View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
- | div.MenuBar {
| + | <li>Observations: <br> |
- | background-color: #0064C8; /* Selected item. */
| + | 09_06: swimming was observed, experiment was continued |
- | color: #FFFFFF;
| + | </ul> |
- | border-bottom: 1px solid ButtonShadow;
| + | <br> |
- | }
| + | |
- | div.MenuBar a,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
- | background-color: #00446b; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover a,
| + | |
- | div.MenuBar ul li a:hover,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
- | background-color: #A3C0E0; /* Selected item. */
| + | |
- | color: #FFFFFF;
| + | |
- | }
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu,
| + | |
- | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
- | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
- | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| + | |
- | }
| + | |
- | /*--------------------------------------------------------------------------------------- Widths */
| + | |
- | /*
| + | |
| | | |
- | /*
| |
- | Menu Bar 1
| |
- | Drop-Down Menu #2
| |
- | */
| |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
| |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
| |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
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- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
| |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
| |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
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- | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
| |
- | width: 14em; /* Drop-down menu width. */
| |
- | }
| |
| | | |
- | /*...............................................................................................*/ | + | <br></td></tr> |
- | /* | + | </table> |
- | Menu Bar 1
| + | |
- | Drop-Down Menu #2
| + | |
- | Side Menu #1
| + | |
- | */
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
| + | |
- | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
- | Keep it sync with the drop-down menu width. */
| + | |
- | }
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
| + | |
- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
| + | |
- | width: 15em; /* Side menu width. */
| + | |
- | }
| + | |
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- | /*
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- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
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- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
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- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
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- | width: 7em; /* Side menu width. */
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- | }
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- | /*...............................................................................................*/
| + | |
- | /*
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- | Menu Bar 1
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- | Drop-Down Menu #2
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- | Side Menu #3
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- | */
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- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
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- | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
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- | Keep it sync with the drop-down menu width. */
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- | }
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- | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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- | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
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- | width: 17em; /* Side menu width. */
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| |
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| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
| + | <tr bordercolor="black" valign="top"> |
| + | <td width="900" bordercolor="black" valign="top"> |
| + | <h2><b>V09_06 </b></h2><br> |
| + | <b>V09_06_1: Observation of the growth of the strains used for EM, BL21 + <i>rfp, +flhDC, +flicC</i>and MG1655</b><br> |
| + | <ul> |
| + | <li>Experiment: <br>In order to gain a higher cell density which is necessary of EM, the growth of the cells in different incubation conditions was observed. The cells were grown in 5 mL LB-broth, in 5 mL LB agar topped with 5 mL LB broth, in 10 mL LB-broth and in 10 mL LB agar topped with 10 mL BL-broth.</li> |
| + | <li>Observations: <br> |
| + | After 5.5 h an OD600 of over 2 in 10 mL LB agar topped with 10 mL BL-broth can be reached but it is suspected, that the expression of flagella is reduced through the shaking in the erlenmyer flask. Light microscopic test have to follow. |
| + | </ul> |
| + | <br> |
| | | |
| + | <b>V09_06_2: Separation assay</b><br> |
| + | <ul> |
| + | <li>Experiment: <br>The strains Δ<i>tar</i> J61002_rfp and Δ<i>tar</i> _tar_QC_18C were dropped in the same ratio on 0.3% tryptone swimming agar plates. </a></li> |
| + | <li>Observations: <br> |
| + | Continuation: 09_08_2 |
| + | </ul> |
| + | <br> |
| | | |
- | </p><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head> | + | <b>V09_06_3: Selection of clones from the “plating on the chloramphenicol containing LB plates step” for sequencing and observation of the swimming behavior</b><br> |
- | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
| + | <ul> |
- | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
| + | <li>Experiment: <br>Clones were selected form the CM plates containing clones form the library. These were again secured on LB agar plates (with CM) and used to inoculate 7 mL LB-broth containing CM..</li> |
- | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
| + | <li>Observations: <br> |
- | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
| + | 09_07: all cultures grew, the minipreparation was conducted and the extracted vectors transformed into fresh BL21 cells. |
- | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
| + | </ul> |
- | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss"> | + | <br> |
- | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
| + | |
- | | + | |
- | | + | |
- | <body class="mediawiki ns-0 ltr page-Team_Goettingen"> | + | |
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- | | + | |
- | | + | |
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- | | + | |
- | | + | |
- | <!-- start content -->
| + | |
- | | + | |
- | | + | |
| | | |
| + | <br></td></tr> |
| + | </table> |
| | | |
| + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
| + | <tr bordercolor="black" valign="top"> |
| + | <td width="900" bordercolor="black" valign="top"> |
| + | <h2><b>V09_07 </b></h2><br> |
| + | <b>V09_07_1: Observation of the swimming behavior of the through the library selection selected clones after they were plates on LB-agar containing chloramphenicol</b><br> |
| + | <ul> |
| + | <li>Experiment: <br>In order to investigate whether the selected clones containing a library vector still swim with approximately the same speed after they have been plated an LB agar containing chloramphenicol they were dropped again on 0.3 % tryptone swimming agar with the respective attractant and incubated over night at 33 °C</li> |
| + | <li>Observations: <br> |
| + | 09_08: The clones still swim as fast! |
| + | </ul> |
| + | <br> |
| | | |
| + | <b>V09_07_2: Retransformation of the extracted plasmids of the selected clones containing the library vectors into fresh BL21 cells</b><br> |
| + | <ul> |
| + | <li>Experiment: <br>In order to determine whether the observed behavior is dependent on the selected clones or on their vectors, the extracted vectors were transformed into new BL21 cells. View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
| + | <li>Observations: <br> |
| + | 09_08: growth was observed on each plate! |
| + | </ul> |
| + | <br> |
| | | |
- | <p></p><div id="header"><img src="http://www.patrickreinke.de/igem/headpicture_2.jpg" alt="Team Goettingen"> | + | <b>V09_07_3: Plating of the selected clones of the 3rd approach of the library selection</b><br> |
- | <br><br></p><div class="MenuBar" id="navi">
| + | <ul> |
- | <ul>
| + | <li>Experiment: <br>View <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">methods</a>.</li> |
- | <li>
| + | <li>Observations: <br> |
- | <a href="https://2012.igem.org/Team:Goettingen" style="color: white;">Home<!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | 09_08: Growth was observed on every plate, no clones were picked and no vectors were sequenced. |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
| + | </ul> |
- | <!--<ul class="DropDownMenu" id="MB1-DDM6">
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- | </ul> -->
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- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
- | </li>
| + | |
- | <li>
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- | <a href="https://2012.igem.org/Team:Goettingen/Team" style="color: white;">Team<!--[if gt IE 6]><!--></a><!--<![endif]-->
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- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | <ul class="DropDownMenu" id="MB1-DDM5">
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Team"><span><span>Members</span></span></a></li>
| + | |
- | <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#advisor"><span><span><div style="text-indent:20px;">⋅ Advisors</div></span></span></a></li> -->
| + | |
- | <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#students"><span><span><div style="text-indent:20px;">⋅ Students</div></span></span></a></li> -->
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/Team#responsibitlities"><span><span><div style="text-indent:20px;">⋅ Responsibilities</div></span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Focus_Groups"><span><span>Focus Groups</span></span></a></li>
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group1"><span><span><div style="text-indent:20px;">⋅ #1 Selection / Swimming</div></span></span></a></li> -->
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group2"><span><span><div style="text-indent:20px;">⋅ #2 Speed Improvement</div></span></span></a></li> -->
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group3"><span><span><div style="text-indent:20px;">⋅ #3 Chemoreceptor</div></span></span></a></li> -->
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#library"><span><span><div style="text-indent:20px;">⋅ Library</div></span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Seminars"><span><span>Seminars</span></span></a></li>
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#announce"><span><span><div style="text-indent:20px;">⋅ Announcements</div></span></span></a></li> -->
| + | |
- | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#presentations"><span><span><div style="text-indent:20px;">⋅ Past Presentations</div></span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Work_Impressions"><span><span>Work Impressions</span></span></a></li>
| + | |
- | </ul>
| + | |
- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
- | </li>
| + | |
- | <li>
| + | |
- | <a href="https://2012.igem.org/Team:Goettingen/Project" style="color: white;">Project<!--[if gt IE 6]><!--></a><!--<![endif]-->
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- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
| + | |
- | <ul class="DropDownMenu" id="MB1-DDM2-SM3">
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Project"><span><span>Our Project</span></span></a></li>
| + | |
- | <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Chemotaxis"><span><span><div style="text-indent:20px;">⋅ Chemotaxis</div></span></span></a></li> -->
| + | |
- | <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Poster"><span><span><div style="text-indent:20px;">⋅ Poster</div></span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Materials"><span><span>Materials</span></span></a>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Methods"><span><span>Methods</span></span></a>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Computational_Data"><span><span>Computational Data</span></span></a>
| + | |
- | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">⋅ Maps</div></span></span></a></li>
| + | |
- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">⋅ Sequences</div></span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Bioinformatical_Tool"><span><span>Bioinformatical Tools</span></span></a>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Lab_Work_Flow"><span><span>Lab Work Flow</span></span></a>
| + | |
- | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">⋅ Golden Lab Rules</div></span></span></a></li>
| + | |
- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">⋅ Daily Check List</div></span></span></a></li> -->
| + | |
- | </ul>
| + | |
- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
- | </li>
| + | |
- | | + | |
- | <li>
| + | |
- | <a href="https://2012.igem.org/Team:Goettingen/Notebook" style="color: white;">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | <ul class="DropDownMenu" id="MB1-DDM2">
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook"><span><span>Overview</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary"><span><span>Summary</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Discussion"><span><span>Discussion</span></span></a></li>
| + | |
- | </ul>
| + | |
- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
- | </li>
| + | |
- | <li>
| + | |
- | <a href="https://2012.igem.org/Team:Goettingen/iGEM" style="color: white;">iGEM<!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
| + | |
- | <ul class="DropDownMenu" id="MB1-DDM6">
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM"><span><span>What is iGEM?</span></span></a></li>
| + | |
- | <!--<li><a href="https://2012.igem.org/Team:Goettingen/BioBrick_System"><span><span>iGEM BioBrick System</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Part_Registry"><span><span>iGEM Part Registry</span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts_Submitted"><span><span>Parts Submitted</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Attributions"><span><span>Attributions</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Saftey"><span><span>Saftey</span></span></a></li>>
| + | |
- | </ul>
| + | |
- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
- | </li>
| + | |
- | <li>
| + | |
- | <a href="https://2012.igem.org/Team:Goettingen/Goettingen" style="color: white;">Göttingen<!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | <ul class="DropDownMenu" id="MB1-DDM5">
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen"><span><span>City</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/University"><span><span>Georg-August-University</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/MPI"><span><span>Max-Planck-Institute</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/S1-Demo_Lab"><span><span>S1-Demo Lab</span></span></a></li>
| + | |
- | | + | |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | </ul>
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- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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- | </li>
| + | |
- | <li style="width: 160px">
| + | |
- | <a href="https://2012.igem.org/Team:Goettingen/Human_Practice" style="color: white;">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | <ul class="DropDownMenu" id="MB1-DDM5">
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice"><span><span>Why Human Practice?</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Public_and_Media"><span><span>Public and Media</span></span></a></li>
| + | |
- | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">⋅ Newspaper</div></span></span></a></li>
| + | |
- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Conference"><span><span><div style="text-indent:20px;">⋅ Conference</div></span></span></a></li>
| + | |
- | </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">⋅ Synthetic Biology Day</div></span></span></a></li> -->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Survey"><span><span>Survey</span></span></a></li>
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- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion"><span><span>Panel Discussion</span></span></a></li>-->
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
| + | |
- | </ul>
| + | |
- | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
- | </li>
| + | |
- | | + | |
- | <li>
| + | |
- | <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
| + | |
- | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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- | <ul class="DropDownMenu" id="MB1-DDM2-SM3">
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Sponsoring"><span><span>Interested in...?</span></span></a></li>
| + | |
- | <!-- <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Sponsors"><span><span>Sponsors</span></span></a></li>
| + | |
- | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Supporters"><span><span>Supporters</span></span></a></li> -->
| + | |
- | </li>
| + | |
- | </ul>
| + | |
- | | + | |
- | </div>
| + | |
- | | + | |
- | | + | |
- | <table>
| + | |
- | <!-- Text body -->
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| + | |
- | <font face="Verdana" size="-1">
| + | |
- | <br>
| + | |
- | Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
| + | |
| <br> | | <br> |
| | | |
| + | <br></td></tr> |
| + | </table> |
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- | <h2><b><a name="week3#2">#1 Selection / Swimming - 19th Week</a></b></h2>
| |
- | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
| |
- | <br>
| |
- | <p align="justify" style="line-height:1.6em">
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- | </p>
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| <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
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- | <h2><b>VX_Y </b></h2><br> | + | <h2><b>V09_08 </b></h2><br> |
- | <b>Titel</b><br> | + | <b>V09_08_1: Swimming behavior of the library: selected clones versus retransformed clones</b><br> |
| <ul> | | <ul> |
- | <li>Experiment: <br>hier text reinschreiben</li> | + | <li>Experiment: <br>Continuation of 09_07_1. Growth could be observed on every plate, single colonies were picked and used to inoculate 1 ml LB broth. Cultures were shaken for approximately 3 h.</li> |
| + | <li>Observations: <br> |
| + | 09_09: there is a huge difference between the swimming behavior of the selected clones and the retransformed clones. |
| </ul> | | </ul> |
- | <br></td></tr>
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- | </table>
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| <br> | | <br> |
- | <body id="top"> | + | |
- | <a href="#top">↑ Return to top</a> | + | <b>V09_08_2: Separation assay</b><br> |
| + | <ul> |
| + | <li>Experiment: <br>Separation of the strains Δ<i>tar</i> J61002_rfp and Δ<i>tar</i> _tar_QC_18C. The agar was cut out at three different positions and the pieces incubated in 1 mL LB broth for approximately 3 h. The cultures were diluted and the 10^-2 and the 10^-4 dilution was plated on plates containing either ampicillin of chloramphenicol. 3 dopes were treated as described above. </li> |
| + | <li>Observations: <br> |
| + | 09_09: In one of three cases (one not evaluable) the Δ<i>tar</i> _tar_QC_18C strain was faster. |
| + | </ul> |
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- | <b>Important pages</b>:<br>
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- | <a href="https://2012.igem.org/Team:Goettingen">Home</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>;
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- | <a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
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- | <a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a>
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- | </td></tr> | + | |
| </table> | | </table> |
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