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| - | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
| + | {{GoettingenHeader|deu=Team:Goettingen/week13-1|eng=Team:Goettingen/week13-1}} |
| - | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
| + | <html> |
| - | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
| + | <table> |
| - | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
| + | |
| - | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
| + | |
| - | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
| + | |
| - | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss">
| + | |
| - | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
| + | |
| | | | |
| - |
| |
| - | <body class="mediawiki ns-0 ltr page-Team_Goettingen">
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| - |
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| - |
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| - |
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| - |
| |
| - | <!-- start content -->
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| - |
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| - |
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| - |
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| - | <style>
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| - | h1.firstHeading { display: none; }
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| - |
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| - | p {text-align: justify;}
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| - |
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| - | a:link { color: #004080; text-decoration: none}
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| - | a:visited { color:##003090; text-decoration: none}
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| - | a:hover { color:#f29400; text-decoration: none}
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| - | a:active { color:#f29400; text-decoration: none}
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| - |
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| - | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
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| - |
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| - | table#team_members { text-align: justify; border: 0; }
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| - | table#team_members h2, table#team_members h3 { clear: both; }
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| - |
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| - |
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| - | /*-----------------------------------------------------------------------------------------------*/
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| - | div.MenuBar ul li ul.DropDownMenu {
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| - | display: none; /* Hides all drop-down menus. */
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| - |
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| - | }
| |
| - | /*
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| - | li:hover works in IE7 and FF2.
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| - | a:hover works in IE6 and FF2.
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| - | a:hover breaks li:hover in FF2.
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| - | */
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| - | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| |
| - | display: none; /* Hides all side menus. */
| |
| - | }
| |
| - | /*------------------------------------------------------------------------------------- Menu Bar */
| |
| - | div.MenuBar {
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| - | font: Verdana;
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| - | height: 30px;
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| - | width: 910px;
| |
| - | /*width: 100%*/
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| - | margin: 0;
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| - | border-top: 0;
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| - | border-right: 0;
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| - | border-left: 0;
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| - | padding: 0;
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| - | background: #00446b;
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| - |
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| - | }
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| - | div.MenuBar ul {
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| - | font: Verdana;
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| - | text-align: center;
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| - | list-style-type: none;
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| - | margin: 0 auto;
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| - | border: 0;
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| - | padding: 0;
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| - | background: #00446b;
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| - | }
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| - | div.MenuBar ul li {
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| - | font: Verdana;
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| - | display: block;
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| - | padding: 0;
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| - | margin: 0;
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| - | font-size: 1.3em;
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| - | float: left;
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| - | background: #00446b;
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| - | text-align: center;
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| - | width: 107px;
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| - | position: relative; /* Sets the positioning context for each drop-down menu. */
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| - | }
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| - |
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| - | div.MenuBar ul li a {
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| - | font: Verdana;
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| - | display: block;
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| - | background: #00446b;
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| - | height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
| |
| - | color: white;
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| - | padding-top: 4px;
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| - | padding-bottom: 4px;
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| - | padding-left: 1em; /* Sets the left space between top-level items. */
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| - | padding-right: 1em; /* Sets the right space between top-level items. */
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| - | text-decoration: none;
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| - | }
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| - |
| |
| - | /*------------------------------------------------------------------------------ Drop-Down Menus */
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| - | div.MenuBar ul li:hover ul.DropDownMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu {
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| - | display: block;
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| - | width: 10em; /* Drop-down menu width.
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| - | Use MenuTailor.css to customize. */
| |
| - | height: 1em;
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| - | padding: 1px; /* Sets the drop-down menu "effective border" width. */
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| - | position: absolute;
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| - | top: 28px; /* Places the drop-down menu under the menu bar.
| |
| - | Keep it sync with the menu bar height. */
| |
| - | left: 0; /* Aligns the drop-down menu to its top-level item. */
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| - | background-color: #A3C0E0; /* Selected item. */
| |
| - | color: #FFFFFF;
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| - |
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
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| - | width: 10em; /* Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | height: 1em;
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| - | padding-left: 0;
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| - | padding-right: 0;
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| - | background-color: #A3C0E0; /* Selected item. */
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| - | color: #FFFFFF;
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| - | }
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| - | ul.DropDownMenu li a span {
| |
| - | display: block;
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| - | padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
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| - | padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
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| - | text-align: right; /* Aligns the >> symbol to the right. */
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| - | }
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| - | ul.DropDownMenu li a span span {
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| - | float: left; /* Aligns the text (back) to the left. */
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| - | font: 12px Verdana; /* Required for IE55. */
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| - | height: 20px;
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| - | color: #FFFFFF;
| |
| - | }
| |
| - | /*----------------------------------------------------------------------------------- Side Menus */
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| |
| - | display: block;
| |
| - | width: 11em; /* Side menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | padding: 1px; /* Sets the side menu "effective border" width. */
| |
| - | position: absolute;
| |
| - | top: -1px; /* Aligns the side menu to its drop-down menu item.
| |
| - | Keep it sync with the side menu "effective border" width. */
| |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| |
| - | width: 11em; /* Keep it sync with the side menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | font: 12px Verdana; /* Required for IE55. */
| |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | }
| |
| - | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| |
| - | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| |
| - | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| |
| - | text-align: left;
| |
| - | font: 12px Verdana; /* Required for IE55. */
| |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width.
| |
| - | Use MenuTailor.css to customize. */
| |
| - | }
| |
| - | /*----------------------------------------------------------------------------- Browser Specific */
| |
| - | * html div.MenuBar ul li a {
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| - | float: left; /* Required for IE55 and IE6.
| |
| - | Breaks O9.
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| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | * html ul.DropDownMenu li a:hover {
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| - | cursor: hand; /* Required for IE55.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | ul.DropDownMenu li a:hover {
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| - | cursor: pointer; /* Required for IE6 and IE7.
| |
| - | Hidding it (* html) from non-IE browsers breaks IE7!
| |
| - | }
| |
| - | * html div.MenuBar a:hover {
| |
| - | text-decoration: none; /* Required for IE55 and IE6.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | * html div.MenuBar ul li table,
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| - | * html div.MenuBar ul li table td {
| |
| - | border: 0; /* Required for IE55 and IE6.
| |
| - | Hidden (* html) from non-IE browsers. */
| |
| - | }
| |
| - | /*------------------------------------------------------------------------------- Default Colors */
| |
| - | div.MenuBar {
| |
| - | background-color: Menu;
| |
| - | border-bottom: 1px solid ButtonShadow;
| |
| - | }
| |
| - | div.MenuBar a {
| |
| - | background-color: Menu; /* Top-level unselected items. */
| |
| - | color: MenuText;
| |
| - | }
| |
| - | div.MenuBar ul li:hover a,
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| - | div.MenuBar ul li a:hover {
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| - | color: #A3C0E0;
| |
| - | background-color: Highlight; /* Top-level selected item. */
| |
| - | color: HighlightText;
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| |
| - | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| |
| - | background-color: Menu; Drop-down menu unselected items.
| |
| - | Sets the drop-down menu "effective background" color. */
| |
| - | color: MenuText;
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| |
| - | background-color: Highlight; /* Drop-down menu selected item. */
| |
| - | color: HighlightText;
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| |
| - | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| |
| - | background-color: Menu; /* Side menu unselected items.
| |
| - | Sets the side menu "effective background" color. */
| |
| - | color: MenuText;
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| |
| - | background-color: Highlight; /* Side menu selected item. */
| |
| - | color: HighlightText;
| |
| - | }
| |
| - | /*-----------------------------------------------------------------------------------------------*/
| |
| - |
| |
| - | /*-------------------------------------------------------------------------------------- General */
| |
| - | body {
| |
| - | background: white;
| |
| - | color: black;
| |
| - | margin: 0;
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| - | border: 0;
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| - | padding: 0;
| |
| - | }
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| - |
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| - |
| |
| - | div.MenuBar {
| |
| - | font: 13px arial, helvetica, sans-serif;
| |
| - | }
| |
| - | div.MenuBar ul {
| |
| - | font: 13px arial, helvetica, sans-serif; /* Required for IE55. */
| |
| - | }
| |
| - | /*--------------------------------------------------------------------------------------- Colors */
| |
| - | div.MenuBar {
| |
| - | background-color: #0064C8; /* Selected item. */
| |
| - | color: #FFFFFF;
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| - | border-bottom: 1px solid ButtonShadow;
| |
| - | }
| |
| - | div.MenuBar a,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
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| - | background-color: #00446b; /* Selected item. */
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| - | color: #FFFFFF;
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| - | }
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| - | div.MenuBar ul li:hover a,
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| - | div.MenuBar ul li a:hover,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| |
| - | background-color: #A3C0E0; /* Selected item. */
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| - | color: #FFFFFF;
| |
| - | }
| |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu,
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| |
| - | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| |
| - | }
| |
| - | /*--------------------------------------------------------------------------------------- Widths */
| |
| - | /*
| |
| - |
| |
| - | /*
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| - | Menu Bar 1
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| - | Drop-Down Menu #2
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
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| - | width: 15.5em; /* Drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
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| - | width: 14em; /* Drop-down menu width. */
| |
| - | }
| |
| - |
| |
| - | /*...............................................................................................*/
| |
| - | /*
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| - | Menu Bar 1
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| - | Drop-Down Menu #2
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| - | Side Menu #1
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
| |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
| |
| - | width: 15em; /* Side menu width. */
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | /*
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| - | Menu Bar 1
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| - | Drop-Down Menu #2
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| - | Side Menu #2
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
| |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
| |
| - | width: 7em; /* Side menu width. */
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - | /*
| |
| - | Menu Bar 1
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| - | Drop-Down Menu #2
| |
| - | Side Menu #3
| |
| - | */
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
| |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| |
| - | Keep it sync with the drop-down menu width. */
| |
| - | }
| |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
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| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
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| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
| |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
| |
| - | width: 17em; /* Side menu width. */
| |
| - | }
| |
| - | /*...............................................................................................*/
| |
| - |
| |
| - | </style>
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| - |
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| - |
| |
| - |
| |
| - | </p><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
| |
| - | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
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| - | <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
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| - | <link rel="shortcut icon" href="https://2012.igem.org/favicon.ico">
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| - | <link rel="search" type="application/opensearchdescription+xml" href="https://2012.igem.org/wiki/opensearch_desc.php" title="2012.igem.org (English)">
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| - | <link title="Creative Commons" type="application/rdf+xml" href="https://2012.igem.org/wiki/index.php?title=Team:Goettingen&action=creativecommons" rel="meta">
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| - | <link rel="alternate" type="application/rss+xml" title="2012.igem.org RSS Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=rss">
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| - | <link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom Feed" href="https://2012.igem.org/wiki/index.php?title=Special:Recentchanges&feed=atom">
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| - |
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| - |
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| - | <body class="mediawiki ns-0 ltr page-Team_Goettingen">
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| - |
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| - |
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| - |
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| - |
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| - | <!-- start content -->
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| - |
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| - |
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| - |
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| - | <p></p><div id="header"><img src="http://www.patrickreinke.de/igem/headpicture_2.jpg" alt="Team Goettingen">
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| - | <br><br></p><div class="MenuBar" id="navi">
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| - | <ul>
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| - | <a href="https://2012.igem.org/Team:Goettingen" style="color: white;">Home<!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <!--<li><a href="https://2012.igem.org/Team:Goettingen/Team#students"><span><span><div style="text-indent:20px;">⋅ Students</div></span></span></a></li> -->
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| - | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/Team#responsibitlities"><span><span><div style="text-indent:20px;">⋅ Responsibilities</div></span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Focus_Groups"><span><span>Focus Groups</span></span></a></li>
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| - | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group1"><span><span><div style="text-indent:20px;">⋅ #1 Selection / Swimming</div></span></span></a></li> -->
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| - | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group2"><span><span><div style="text-indent:20px;">⋅ #2 Speed Improvement</div></span></span></a></li> -->
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| - | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/groups#group3"><span><span><div style="text-indent:20px;">⋅ #3 Chemoreceptor</div></span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Seminars"><span><span>Seminars</span></span></a></li>
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| - | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#announce"><span><span><div style="text-indent:20px;">⋅ Announcements</div></span></span></a></li> -->
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| - | <!--<li><li><a href="https://2012.igem.org/Team:Goettingen/seminars#presentations"><span><span><div style="text-indent:20px;">⋅ Past Presentations</div></span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Work_Impressions"><span><span>Work Impressions</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Project"><span><span>Our Project</span></span></a></li>
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| - | <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Chemotaxis"><span><span><div style="text-indent:20px;">⋅ Chemotaxis</div></span></span></a></li> -->
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| - | <!--<li></li><li><a href="https://2012.igem.org/Team:Goettingen/Poster"><span><span><div style="text-indent:20px;">⋅ Poster</div></span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Materials"><span><span>Materials</span></span></a>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Methods"><span><span>Methods</span></span></a>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Computational_Data"><span><span>Computational Data</span></span></a>
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| - | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">⋅ Maps</div></span></span></a></li>
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| - | </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">⋅ Sequences</div></span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Bioinformatical_Tool"><span><span>Bioinformatical Tools</span></span></a>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Project/Lab_Work_Flow"><span><span>Lab Work Flow</span></span></a>
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| - | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Maps"><span><span><div style="text-indent:20px;">⋅ Golden Lab Rules</div></span></span></a></li>
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| - | </li><li><a href="https://2012.igem.org/Team:Goettingen/Sequences"><span><span><div style="text-indent:20px;">⋅ Daily Check List</div></span></span></a></li> -->
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| - | <a href="https://2012.igem.org/Team:Goettingen/Notebook" style="color: white;">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary"><span><span>Summary</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Notebook/Discussion"><span><span>Discussion</span></span></a></li>
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| - | </ul>
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| - | <a href="https://2012.igem.org/Team:Goettingen/iGEM" style="color: white;">iGEM<!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM"><span><span>What is iGEM?</span></span></a></li>
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| - | <!--<li><a href="https://2012.igem.org/Team:Goettingen/BioBrick_System"><span><span>iGEM BioBrick System</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Part_Registry"><span><span>iGEM Part Registry</span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts_Submitted"><span><span>Parts Submitted</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Attributions"><span><span>Attributions</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/iGEM/Saftey"><span><span>Saftey</span></span></a></li>>
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| - | <a href="https://2012.igem.org/Team:Goettingen/Goettingen" style="color: white;">Göttingen<!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen"><span><span>City</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/University"><span><span>Georg-August-University</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/MPI"><span><span>Max-Planck-Institute</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Goettingen/S1-Demo_Lab"><span><span>S1-Demo Lab</span></span></a></li>
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| - | <li style="width: 160px">
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| - | <a href="https://2012.igem.org/Team:Goettingen/Human_Practice" style="color: white;">Human Practice<!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice"><span><span>Why Human Practice?</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Public_and_Media"><span><span>Public and Media</span></span></a></li>
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| - | <!--</li><li><a href="https://2012.igem.org/Team:Goettingen/Newspaper"><span><span><div style="text-indent:20px;">⋅ Newspaper</div></span></span></a></li>
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| - | </li><li><a href="https://2012.igem.org/Team:Goettingen/Conference"><span><span><div style="text-indent:20px;">⋅ Conference</div></span></span></a></li>
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| - | </li><li><a href="https://2012.igem.org/Team:Goettingen/Synthetic_Biology_Day"><span><span><div style="text-indent:20px;">⋅ Synthetic Biology Day</div></span></span></a></li> -->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Survey"><span><span>Survey</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Panel_Discussion"><span><span>Panel Discussion</span></span></a></li>-->
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Flash_coli"><span><span>Flash Coli</span></span></a></li>
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| - | </ul>
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| - | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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| - | <a href="https://2012.igem.org/Team:Goettingen/Sponsoring" style="color: white;">Sponsoring</a>
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| - | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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| - | <ul class="DropDownMenu" id="MB1-DDM2-SM3">
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Sponsoring"><span><span>Interested in...?</span></span></a></li>
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| - | <!-- <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Sponsors"><span><span>Sponsors</span></span></a></li>
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| - | <li><a href="https://2012.igem.org/Team:Goettingen/Human_Practice/Supporters"><span><span>Supporters</span></span></a></li> -->
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| - | </li>
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| - | <!-- Text body -->
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| - | <br>
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| - | Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
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| | </p> | | </p> |
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| | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> | | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
| | <tr bordercolor="black" valign="top"> | | <tr bordercolor="black" valign="top"> |
| | <td width="900" bordercolor="black" valign="top"> | | <td width="900" bordercolor="black" valign="top"> |
| - | <h2><b>VX_Y </b></h2><br> | + | <h2><b>V07_23 </b></h2><br> |
| - | <b>Titel</b><br> | + | <b>Fluorescence diffusion test</b><br> |
| | <ul> | | <ul> |
| - | <li>Experiment: <br>hier text reinschreiben</li> | + | <li>Experiment: <br>Tryptone solution was supplemented with fluorescin to identify the optimal fluorescin concentration for the visualization of diffusion of the chemoattractant soaked into the whatman paper. Therefore, plates with M9 agar were prepared, the whatman paper in the middle of each plate was soaked with tryptone and in addition a variety of amounts of fluorescin. All that was done for several incubation temperatures (RT, 4°C, and 37°C)</li> |
| - | </ul> | + | <li>Observations & Results:<br> |
| | + | After two hours, no diffusion was visible on any plate at all.</ul> |
| | <br></td></tr> | | <br></td></tr> |
| | </table> | | </table> |
| - | <br>
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| - | <body id="top">
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| - | <a href="#top">↑ Return to top</a>
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| - | <br>
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| - | <br>
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| - | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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| - | <br>
| |
| - | </td>
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| | | | |
| | + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
| | + | <tr bordercolor="black" valign="top"> |
| | + | <td width="900" bordercolor="black" valign="top"> |
| | + | <h2><b>V07_24 </b></h2><br> |
| | + | <b>Separation approach</b><br> |
| | + | <ul> |
| | + | <li>Experiment: <br>Equimolar mixtures of a great variety of strains with a lot of different plasmids were dropped onto M9- and tryptone-agar plates. After chemotaxis is visible on the plates, the bacteria from the most outer front will be picked and resuspended. This resuspension will be streaked out onto new plates and counted, to determine the ratio of the mixed strains. This information can be used, to identify the faster and more motile strains.</li> |
| | + | <li>Observations & Results:<br> |
| | + | After two days, the plates showed no significant swimming. After one more day, swimming was detectable for BL21 and some DH10B strains. Only BL21 showed notable chemotaxis, whereas the kind of transformed plasmid doesn't seam to have any major influence. All BL21 straines showed more or less the same amount of chemotaxis.</ul> |
| | + | <br></td></tr> |
| | + | </table> |
| | | | |
| - | <table bordercolor="black" border="1 px" width="600 px"><tr><td> | + | |
| - | <b>Important pages</b>:<br> | + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
| - | <a href="https://2012.igem.org/Team:Goettingen">Home</a>;
| + | <tr bordercolor="black" valign="top"> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Team">Team</a>; | + | <td width="900" bordercolor="black" valign="top"> |
| - | <a href="https://igem.org/Team.cgi?year=2012">Official Team Profile</a>; | + | <h2><b>V07_25 </b></h2><br> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Project">Project</a>; | + | <b>Repetition of the fluorescin diffusion assay (V07_23)</b><br> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Parts">Parts submitted to the Registry</a>;
| + | <ul> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Modeling">Modeling</a>; | + | <li>Experiment: <br>V07_25: A variety of dilutions (1:5, 1:20, 1:50, and 1:100) of the fluorescin stock solution with aspartate solution were produced. These solutions were incorporated into the whatman paper and plated onto the M9 agar plates. Now, a picture was taken from time to time to identify both, the perfect concentration and duration for diffusion. Everything was incubated at 37°C</li> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Notebook</a>;
| + | <li>Observations & Results:<br> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Saftey">Saftey</a>;
| + | After 20h, the dilution levels 1:20 and 1:50 showed good results. Not everything, but more then the half of the plates were taken by diffusion.</ul> |
| - | <a href="https://2012.igem.org/Team:Goettingen/Attributions">Attributions</a> | + | <br></td></tr> |
| - | </td></tr> | + | |
| | </table> | | </table> |
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| | + | <table cellpadding="20 px" border="1" bordercolor="black" valign="top"> |
| | + | <tr bordercolor="black" valign="top"> |
| | + | <td width="900" bordercolor="black" valign="top"> |
| | + | <h2><b>V07_26 </b></h2><br> |
| | + | <b>Preparation of glycerol stocks of transformed cells</b><br> |
| | + | <ul> |
| | + | <li>Experiment: <br>Strains of BL21 and DH10B with a variety of plasmids (mostly different promotor strengths and Tar or flhDC ORF downstream) were converted into glycerol stocks and stored at -80°C.</li> |
| | + | </ul> |
| | + | <br></td></tr> |
| | + | </table> |
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| | + | <br> |
| | + | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> |
| | + | <br> |
| | + | </td> |
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| | </html> | | </html> |
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