Team:TMU-Tokyo

From 2012.igem.org

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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.(1)
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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.(1)
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We planed to create E.coli with an ability to detect and detoxify formaldehyde., named Chef Ant E.coli.
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We planed to create E.coli with an ability to detect and detoxify formaldehyde. named Chef Ant E.coli.
About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP.  
About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP.  
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We plan to overexpress two enzymes in E.coli.
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Moreover we plan to overexpress two enzymes in Chef Ant E.coli.
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First,formaldehyde dehydrogenase decomposes formaldehyde to formic acid. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida.
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First, formaldehyde dehydrogenase decomposes formaldehyde to formic acid. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida.
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Second,formic acid dehydrogenase converts formic acid to CO2 and H2O. The gene of formic acid dehydrogenase is from Methylobacterium extorquens.  
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Second, formic acid dehydrogenase converts formic acid to CO2 and H2O. The gene of formic acid dehydrogenase is from Methyl bacterium extorquens.  
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Revision as of 01:27, 7 September 2012

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Our team project Chef Ant E.coli

Project Abstract


Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.(1) We planed to create E.coli with an ability to detect and detoxify formaldehyde. named Chef Ant E.coli. About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP. Moreover we plan to overexpress two enzymes in Chef Ant E.coli. First, formaldehyde dehydrogenase decomposes formaldehyde to formic acid. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida. Second, formic acid dehydrogenase converts formic acid to CO2 and H2O. The gene of formic acid dehydrogenase is from Methyl bacterium extorquens.

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