Team:TMU-Tokyo/Project

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{{team:TMU-Tokyo/header}}
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace. 
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<div class="container_12">
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<Table border="0" Width="900">
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<Strong>Abstract</Strong><Br>
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<Hr>
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Formaldehyde is a common harmful chemical, and it has a bad effect in
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relatively low concentration. (For example, in agricultural chemicals,
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in disinfectant at hospitals and in paint of building materials) Also,
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since fromaldehyde is mass-produced in factories, it is highly
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possible to exceed over the permissible amount in the environment.
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These days, in Japan, <A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">the detection of formaldehyde in Tone river became an issue.</A><Br>
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<Br>
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<Br>
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<Strong>Device1; Sensing formaldehyde and then producing GFP</Strong><Br>
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<Hr>
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In frmRAB operon, which origin is chromosome of Escherichia coli, frmAB is normally repressed by frmR. But, when formaldehyde exists, frmR loses its inhibitory. This device is composed of frmR gene, its promoter and GFP gene.<Br>
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<Br>
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<Br>
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<Strong>Device2; Producing formaldehyde dehydrogenase, changing formaldehyde to formate</Strong><Br>
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<i>Escherichia coli</i> has original formaldehyde dehydrogenase, but it depends on glutathione (existence). Instead of it, we found glutathione-independent formaldehyde dehydrogenase in <i>Pseudomonas putida</i>. This device is composed of its gene and constitutive promoter.<Br>
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<Br>
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<Br>
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<Strong>Device3; Producing formate dehydrogenase, changing formate to water and carbon dioxide</Strong><Br>
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<Hr>
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This formate dehydrogenase gene is derived from <i>Methylobacterium extorquens</i>. As formaldehyde dehydrogenase, <i>Escherichia coli</i> has similar function gene of this gene. But it has too long length to insert high copy plasmid.
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<i>Escherichia coli</i> has a gene that has the similar function as a formate dehydrogenase but it is too long to be inserted into the high copy plasmid. Therefore, we used the <i>Methylobacterium extorquens</i>’s one.
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This device is composed of its gene and constitutive promoter.<Br>
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<Br>
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<Br>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:TMU-Tokyo|Home]]
 
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!align="center"|[[Team:TMU-Tokyo/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=TMU-Tokyo Official Team Profile]
 
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!align="center"|[[Team:TMU-Tokyo/Project|Project]]
 
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!align="center"|[[Team:TMU-Tokyo/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:TMU-Tokyo/Modeling|Modeling]]
 
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!align="center"|[[Team:TMU-Tokyo/Notebook|Notebook]]
 
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!align="center"|[[Team:TMU-Tokyo/Safety|Safety]]
 
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!align="center"|[[Team:TMU-Tokyo/Attributions|Attributions]]
 
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|}
 
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</Td>
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== '''Overall project''' ==
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Revision as of 05:55, 3 September 2012

Abstract
Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.


Device1; Sensing formaldehyde and then producing GFP
In frmRAB operon, which origin is chromosome of Escherichia coli, frmAB is normally repressed by frmR. But, when formaldehyde exists, frmR loses its inhibitory. This device is composed of frmR gene, its promoter and GFP gene.


Device2; Producing formaldehyde dehydrogenase, changing formaldehyde to formate
Escherichia coli has original formaldehyde dehydrogenase, but it depends on glutathione (existence). Instead of it, we found glutathione-independent formaldehyde dehydrogenase in Pseudomonas putida. This device is composed of its gene and constitutive promoter.


Device3; Producing formate dehydrogenase, changing formate to water and carbon dioxide
This formate dehydrogenase gene is derived from Methylobacterium extorquens. As formaldehyde dehydrogenase, Escherichia coli has similar function gene of this gene. But it has too long length to insert high copy plasmid. Escherichia coli has a gene that has the similar function as a formate dehydrogenase but it is too long to be inserted into the high copy plasmid. Therefore, we used the Methylobacterium extorquens’s one. This device is composed of its gene and constitutive promoter.

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