Team:HokkaidoU Japan/Notebook/plastic Week 8
From 2012.igem.org
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==Colony PCR== | ==Colony PCR== | ||
<p> | <p> | ||
+ | The length of PhaA on pSB1C3 and PhaB on pSB1C3 were confirmed by colony PCR.<br/>The result showed PhaA and pSB1C3 were ligated correctly. | ||
+ | </p> | ||
+ | ==Liquid culture== | ||
+ | <p> | ||
+ | Cultivation of bacteria holds RBS (B0034) was started. | ||
</p> | </p> | ||
+ | |||
</div></div> | </div></div> |
Revision as of 13:27, 31 August 2012
Contents |
August 20th
Digestion
A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </br>
DNA solution PhaC | 12 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 4 ul |
Total | 20 ul |
A digestion to divide PhaA and XbaI site and SpeI site.</ br>
DNA solution PhaA | 7 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 9 ul |
Total | 20 ul |
A digestion to divide PhaB and XbaI site and SpeI site.</ br>
DNA solution PhaB | 7 ul |
XbaI | 1 ul |
SpeI | 1 ul |
10xM buffer | 2 ul |
DW | 9 ul |
Total | 20 ul |
August 21th
August 22th
August 24th
Colony PCR
August 25th
Colony PCR
The length of PhaA on pSB1C3 and PhaB on pSB1C3 were confirmed by colony PCR.
The result showed PhaA and pSB1C3 were ligated correctly.
Liquid culture
Cultivation of bacteria holds RBS (B0034) was started.
August 26th
Mini prep of DNA