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Team:University College London/Protocols/ColPic
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<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} | <noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} | ||
== Picking Colonies ==</noinclude> | == Picking Colonies ==</noinclude> | ||
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'''Step 1 – Creating culture media:''' In a sterile environment, set up X numbers of falcons, each with 5mls of media. | '''Step 1 – Creating culture media:''' In a sterile environment, set up X numbers of falcons, each with 5mls of media. | ||
| - | '''Step 2 | + | '''Step 2 - Inoculating Colonies into a Selective Broth::''' Add Yul of antibiotic to reach desired antibiotic concentration. |
Latest revision as of 15:21, 30 August 2012
Picking Colonies
Step 1 – Creating culture media: In a sterile environment, set up X numbers of falcons, each with 5mls of media.
Step 2 - Inoculating Colonies into a Selective Broth:: Add Yul of antibiotic to reach desired antibiotic concentration.
(For Ampicillin this is 50ug/ml, For Kanamycin it is 25ug/ml, for Tetracycline it is 15ug/ml, and for Chloramphenicol it is 25ug/ml)
Step 4 – Selecting a Colony: Select a clear, isolated colony and using an inoculation hoop scoop up a colony onto the tip. Deposit in the falcon tube
Step 5 - Culture: Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours.
