Team:HokkaidoU Japan/Notebook/plastic Week 7

From 2012.igem.org

(Difference between revisions)
(→PCR)
(→PCR)
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===PCR===
===PCR===
<p>
<p>
-
A PCR of pGEM to get linear Pha A and PhaB using Laury primer.  
+
A PCR of pGEM to get linear PhaA and PhaB using Laury primer and X-PhaA-up-F, S-PhaA-dw-R, X-PhaB-up-F, S-PhaB-dw-R.  
{|class="hokkaidou-table-digestion"
{|class="hokkaidou-table-digestion"
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   |-
   |-
   |down primer(10mM)
   |down primer(10mM)
-
  |1 ul
 
-
  |-
 
-
　| KOD plus Neo
 
   |1 ul
   |1 ul
   |-
   |-
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   |2mM sNTPs
   |2mM sNTPs
   |5 ul
   |5 ul
 +
  |-
 +
  |KOD plus neo
 +
  |1ul
   |-
   |-
   |10xPCR buffer
   |10xPCR buffer
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   |-
   |-
   |}
   |}
 +

Revision as of 13:32, 26 August 2012

Retrieved from "http://2012.igem.org/Team:HokkaidoU_Japan/Notebook/plastic_Week_7"