Revision as of 19:18, 16 August 2012

Labjournal

Start of our WET LAB time.
Generating new competent E.coli KRX cells.
Cultiviation of Xanthomonas campestris B100 and E. coli BL21(DE3). The bacterial strains we got from a working group at our University. After cultivation we isolated the genomic DNA. The DNA was needed as template for PCRs to purify the wanted laccase ORFs.
Sending requests to working groups for different plasmids , which have already worked with laccases and described them in their papers. Unfortunately just one answer came back (thanks a lot to them).So we got a vector with the laccase-ORF CotA from Bacillus pumilus ATCC7061 from the Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials in Switzerland.

weekly seminar:

Do we want to order strains of Trametes versicolor and Trametes villosa?
Gathering information about signal sequences in yeast
Decision to create a database, so that we can easily number and inscribe our lab results
Decision to arrange a summer school for pupils in their last year before the final exams
Discussion about how to meet a member of the german Bundestag (the german parliament)

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 [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14]
 [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15]
+[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16]
+[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17]
+[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18]
 ==Week 1 (04/30 - 05/06/12)==