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Team:HokkaidoU Japan/Notebook/plastic protocols
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| + | ===Polymer extraction and purification=== | ||
| + | <div> | ||
| + | <p> | ||
| + | 1. Move dried up bacteria into test tube. | ||
| + | 2. Break up them to separate and add 10ml chloroform. | ||
| + | 3. Incubate for 48hour at 60 | ||
| + | o | ||
| + | C. | ||
| + | 4. Make it filtered through PTFE and move it into another test tube. | ||
| + | 5. Volatilize organic solvent by exposing air and separate polymer. | ||
| + | 6. Add 5ml hexane and voltex for a minute. After centrifuging (1,500rpm, 10min), remove the clear layer. | ||
| + | 7. Volatilize chloroform by exposing air again. | ||
| + | 8. Add 5ml MtOH, voltex for a minute, centrifuge (1,500rpm, 10min), and remove the clear layer. | ||
| + | </p> | ||
| + | </div></div> | ||
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| + | <div class="hokkaidou-notebook-daily"> | ||
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Revision as of 11:28, 5 August 2012
Polymer extraction and purification
1. Move dried up bacteria into test tube. 2. Break up them to separate and add 10ml chloroform. 3. Incubate for 48hour at 60 o C. 4. Make it filtered through PTFE and move it into another test tube. 5. Volatilize organic solvent by exposing air and separate polymer. 6. Add 5ml hexane and voltex for a minute. After centrifuging (1,500rpm, 10min), remove the clear layer. 7. Volatilize chloroform by exposing air again. 8. Add 5ml MtOH, voltex for a minute, centrifuge (1,500rpm, 10min), and remove the clear layer.
