Team:University College London/LabBook

From 2012.igem.org

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<div class="experiment">Expt 4.1 - Transformation of Key Aggregation Module BioBricks</div>
<div class="experiment">Expt 4.1 - Transformation of Key Aggregation Module BioBricks</div>
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<div class="experimentContent">4.1 will be transforming the Curli Cluster, Green Fluorescence Protein and Ribosome Binding Site BioBricks into our W3100 competent cell line. Transformed cells will be cultured under the selective pressure of antibiotic, after which the plasmids are purified and the presence of the correct BioBricks will be diagnosed with an Analytical Restriction Enzyme Digest and Gel Electrophoresis.  </div>
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<div class="experimentContent">4.1 will be transforming the Curli Cluster, Green Fluorescence Protein and Ribosome Binding Site BioBricks into our W3100 competent cell line. Transformed cells will be cultured under the selective pressure of antibiotic, after which the plasmids are purified and the presence of the correct BioBricks will be diagnosed with an Analytical Restriction Enzyme Digest and Gel Electrophoresis.   
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| class="wikitable sortable collapsible" border="1"
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|+ Sortable and collapsible table
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|-
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! scope="col" | Alphabetic
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! scope="col" | Numeric
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! scope="col" | Date
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! scope="col" class="unsortable" | Unsortable
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|-
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| d || 20 || 2008-11-24 || This
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|-
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| b || 8 || 2004-03-01 || column
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|-
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| a || 6 || 1979-07-23 || cannot
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|-
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| c || 4.2 || 1492-12-08 || be
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|-
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| e || 0 || 1601-08-13 || sorted.
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|
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</div>
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{{:Team:University_College_London/templates/foot}}

Revision as of 16:32, 30 July 2012

Expt 4.1 - Transformation of Key Aggregation Module BioBricks
4.1 will be transforming the Curli Cluster, Green Fluorescence Protein and Ribosome Binding Site BioBricks into our W3100 competent cell line. Transformed cells will be cultured under the selective pressure of antibiotic, after which the plasmids are purified and the presence of the correct BioBricks will be diagnosed with an Analytical Restriction Enzyme Digest and Gel Electrophoresis.

| class="wikitable sortable collapsible" border="1" |+ Sortable and collapsible table |- ! scope="col" | Alphabetic ! scope="col" | Numeric ! scope="col" | Date ! scope="col" class="unsortable" | Unsortable |- | d || 20 || 2008-11-24 || This |- | b || 8 || 2004-03-01 || column |- | a || 6 || 1979-07-23 || cannot |- | c || 4.2 || 1492-12-08 || be |- | e || 0 || 1601-08-13 || sorted. |


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