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Team:BostonU/Clotho
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This section aims to describe how we have been using Clotho in our project. We divided the content into the Clotho apps we used, giving a brief explanation of how each app works and examples of situations in which they were useful for our project. | This section aims to describe how we have been using Clotho in our project. We divided the content into the Clotho apps we used, giving a brief explanation of how each app works and examples of situations in which they were useful for our project. | ||
We hope you can all get good ideas on how to use Clotho on your projects and take advantage of this amazing tool!</p> | We hope you can all get good ideas on how to use Clotho on your projects and take advantage of this amazing tool!</p> | ||
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Revision as of 19:39, 23 July 2012
Clotho and Eugene
Clotho
Introduction
Clotho
Spreadit Oligo
Clotho
Bull Trowell
Bull Trowel is a very useful app for adding large amounts of Parts, Oligos, Vectors, Plasmids and etc from an excel sheet to Clotho`s inventory. In our case, we had a list of almost 200 primers that we designed in an Excel sheet that we wanted to include in our inventory. All of them could be added much faster and with lower mistake rate by using Bull Trowel. Below, there is an example of the interface of the app while we were adding our oligos and the Excel sheet from which we took the oligo sequences.
Also, the description section helps to give meaning to the nickname and makes the inventory more user-friendly.
Clotho
Sequence Viewer
Sequence Viewer is a very useful app to analyze sequences that are in the inventory . It allows us not only to view the sequences of DNA, but also to interact with it by highlighting specific portions that we are interested in. For example, we used sequence viewer to find and highlight restriction and fusion sites within the sequence.
Clotho
Spreadit Feature
Spreadit Feature was a very useful tool while we were designing our MoClo primers. When we create primers for moclo, we need to make sure the genes for which we create the primers do not have certain restriction sites within them where our moclo enzymes will cut. We thus use the spreadit feature app to create features such as restriction sites by entering the sequence into the data base so that when viewing the gene sequence in sequence viewer, we can look for these restriction sites. Also, Spreadit Feature enabled us to create MoClo fusion sites as features to be analyzed in our sequences so we can better predict how and where ligation in MoClo reaction is occurring.
