Team:TMU-Tokyo/Project

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<Br>What's Chef Ant E. coli ?<Br>
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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.(1)<Br>
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Our project <b>Chef Ant E. coli</b> is to append abilities to detoxify formaldehyde to <i>Escherichia coli</i>.<Br>
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Following is a simple explanation of our project. If you want more academic explanation, visit <A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_abstract">Abstract</A> or each device page.<Br>
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We planed to create E.coli which removes such a hazardous material, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.
 
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<b>■Background</b><Br>
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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)<Br>
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Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.<Br>
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<b>■Devices</b><Br>
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We planed to create E.coli which removes such a hazardous material, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.<Br>
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More genetic information about <b>Chef Ant E. coli</b> is available in <A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_abstract"><b>project abstract</b></A>.<Br>
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1. Tonegawa's formaldehyde levels spike ― <A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">DAILY YOMIURI ONLINE</A><Br><Br><Br><Br><Br>
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fig.1<Br>
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This is the pathway of formaldehyde visualization and removal.
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Device1; <p class="description"><Strong>Sensing formaldehyde and then producing GFP</Strong><Br>
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In <i>frmRAB</i> operon, which origin is chromosome of <i>Escherichia coli</i>, <i>frmAB</i> is normally repressed by frmR. But, when formaldehyde exists, <i>frmR</i> loses its inhibitory(2). This device is composed of <i>frmR</i> gene, its promoter and GFP gene.</p><Br>
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2. <Strong>Christopher D. Herring and Frederick R. Blattner.</Strong> 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator <i>frmR</i>[<A Href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC522192/">PubMed</A>]</p>
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<IMG Src="https://static.igem.org/mediawiki/2012/1/10/Chef_ant_ecoli.png"><Br>
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Image of <b>Chef Ant E. coli</b>.
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Device2; <p class="description"><Strong>Producing formaldehyde dehydrogenase, changing formaldehyde to formate</Strong><Br>
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<i>Escherichia coli</i> has original formaldehyde dehydrogenase, but it depends on glutathione (existence). Instead of it, we found glutathione-independent formaldehyde dehydrogenase in <i>Pseudomonas putida</i>. This device is composed of its gene and constitutive promoter.<Br></p>
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<b>■Tonegawa's formaldehyde levels spike</b><Br>
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[<A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">DAILY YOMIURI ONLINE</A>]<Br>
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Project Top  <A Href="Project_abstract">Abstract</A>  <A Href="Project_device1">Device1  <A Href="Project_device2">Device2</A>  <A Href="Project_device3">Device3</A><Br><Br></p></Center>
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Device3; <p class="description"><Strong>Producing formate dehydrogenase, changing formate to water and carbon dioxide</Strong><Br>
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This formate dehydrogenase gene is derived from <i>Methylobacterium extorquens</i>. As formaldehyde dehydrogenase, <i>Escherichia coli</i> has similar function gene of this gene. But it has too long length to insert high copy plasmid.
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<i>Escherichia coli</i> has a gene that has the similar function as a formate dehydrogenase but it is too long to be inserted into the high copy plasmid. Therefore, we used the <i>Methylobacterium extorquens</i>’s one.
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This device is composed of its gene and constitutive promoter.</p><Br>
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Latest revision as of 03:02, 27 September 2012

 


What's Chef Ant E. coli ?


 
 

Our project Chef Ant E. coli is to append abilities to detoxify formaldehyde to Escherichia coli.

Following is a simple explanation of our project. If you want more academic explanation, visit Abstract or each device page.





■Background
Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)
Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.


■Devices
We planed to create E.coli which removes such a hazardous material, named Chef Ant E.coli. It is appended 3 devices and gets removal ability of formaldehyde.
More genetic information about Chef Ant E. coli is available in project abstract.




Image of Chef Ant E. coli.

■Tonegawa's formaldehyde levels spike
[DAILY YOMIURI ONLINE]

 

Project Top Abstract Device1 Device2 Device3

Retrieved from "http://2012.igem.org/Team:TMU-Tokyo/Project"