Team:TMU-Tokyo/Project

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<Br>What's Chef Ant E. coli ?<Br>
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Our project <b>Chef Ant E. coli</b> is to append abilities to detoxify formaldehyde to <i>Escherichia coli</i>.<Br>
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Following is a simple explanation of our project. If you want more academic explanation, visit <A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_abstract">Abstract</A> or each device page.<Br>
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<Strong>Abstract</Strong><Br>
 
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Formaldehyde is a common harmful chemical, and it has a bad effect in
 
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relatively low concentration. (For example, in agricultural chemicals,
 
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in disinfectant at hospitals and in paint of building materials) Also,
 
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since fromaldehyde is mass-produced in factories, it is highly
 
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possible to exceed over the permissible amount in the environment.
 
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These days, in Japan, <A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">the detection of formaldehyde in Tone river became an issue.</A><Br>
 
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<Strong>Device1; Sensing formaldehyde and then producing GFP</Strong><Br>
 
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In frmRAB operon, which origin is chromosome of Escherichia coli, frmAB is normally repressed by frmR. But, when formaldehyde exists, frmR loses its inhibitory. This device is composed of frmR gene, its promoter and GFP gene.<Br>
 
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<Strong>Device2; Producing formaldehyde dehydrogenase, changing formaldehyde to formate</Strong><Br>
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<b>■Background</b><Br>
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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)<Br>
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<i>Escherichia coli</i> has original formaldehyde dehydrogenase, but it depends on glutathione (existence). Instead of it, we found glutathione-independent formaldehyde dehydrogenase in <i>Pseudomonas putida</i>. This device is composed of its gene and constitutive promoter.<Br>
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Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.<Br>
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<Strong>Device3; Producing formate dehydrogenase, changing formate to water and carbon dioxide</Strong><Br>
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<b>■Devices</b><Br>
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We planed to create E.coli which removes such a hazardous material, named <Strong>Chef Ant E.coli</Strong>. It is appended 3 devices and gets removal ability of formaldehyde.<Br>
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This formate dehydrogenase gene is derived from <i>Methylobacterium extorquens</i>. As formaldehyde dehydrogenase, <i>Escherichia coli</i> has similar function gene of this gene. But it has too long length to insert high copy plasmid.
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More genetic information about <b>Chef Ant E. coli</b> is available in <A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_abstract"><b>project abstract</b></A>.<Br>
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<i>Escherichia coli</i> has a gene that has the similar function as a formate dehydrogenase but it is too long to be inserted into the high copy plasmid. Therefore, we used the <i>Methylobacterium extorquens</i>’s one.
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This device is composed of its gene and constitutive promoter.<Br>
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<IMG Src="https://static.igem.org/mediawiki/2012/1/10/Chef_ant_ecoli.png"><Br>
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Image of <b>Chef Ant E. coli</b>.
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<b>■Tonegawa's formaldehyde levels spike</b><Br>
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[<A Href="http://www.yomiuri.co.jp/dy/national/T120521004335.htm">DAILY YOMIURI ONLINE</A>]<Br>
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Project Top  <A Href="Project_abstract">Abstract</A>  <A Href="Project_device1">Device1  <A Href="Project_device2">Device2</A>  <A Href="Project_device3">Device3</A><Br><Br></p></Center>
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Latest revision as of 03:02, 27 September 2012

 


What's Chef Ant E. coli ?


 
 

Our project Chef Ant E. coli is to append abilities to detoxify formaldehyde to Escherichia coli.

Following is a simple explanation of our project. If you want more academic explanation, visit Abstract or each device page.





■Background
Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)
Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.


■Devices
We planed to create E.coli which removes such a hazardous material, named Chef Ant E.coli. It is appended 3 devices and gets removal ability of formaldehyde.
More genetic information about Chef Ant E. coli is available in project abstract.




Image of Chef Ant E. coli.

■Tonegawa's formaldehyde levels spike
[DAILY YOMIURI ONLINE]

 

Project Top Abstract Device1 Device2 Device3

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