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Team:Valencia/BG-11Wl
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<li>1,2 g/l de ammonium Iron (III) citrate brown.</li> | <li>1,2 g/l de ammonium Iron (III) citrate brown.</li> | ||
<li>1,2 g/l de citric acid anhydrous.</li> | <li>1,2 g/l de citric acid anhydrous.</li> | ||
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| - | <li>Concentration trace metal 1000X*</li> | + | <li>Concentration trace metal 1000X*:</li> |
<ul style="list-style-type: square"> | <ul style="list-style-type: square"> | ||
<li>2,86 g/l de H<sub>3</sub>BO<sub>4</sub>.</li> | <li>2,86 g/l de H<sub>3</sub>BO<sub>4</sub>.</li> | ||
Latest revision as of 02:45, 27 September 2012
BG-11 for Wildtype in medium liquid.
Reagents and Materials
- Concentration BG-11 200X*:
- 8 g/l de K2HPO4•3H2O.
- 8 g/l de Na2CO3.
- 15 g/l de MgSO4•7H2O.
- 0,2 g/l de Na2EDTA.
- 7,2 g/l de CaCl2•2H2O.
- 1,2 g/l de ammonium Iron (III) citrate brown.
- 1,2 g/l de citric acid anhydrous.
- Concentration trace metal 1000X*:
- 2,86 g/l de H3BO4.
- 1,81 g/l de MnCl2•4H2O.
- 222 mg/l de ZnSO4•7H2O.
- 390 mg/l de Na2MoO4•2H2O.
- 79 mg/l de CuSO4•5H20.
- 49 mg/l de CoCl2•6H2O.
Protocol
- Add all the compounds for the BG-11 in an Erlenmeyer flask with 1 liter of distilled water and shake at room temperature until all salts are dissolved.
- Add all the compounds for the trace metal in an Erlenmeyer flask with 1 liter of distilled water and shake at room temperature until all salts are dissolved.
- In 1 liter of distilled water, put 1 Ml of trace metal and 5 ml of BG-11 and add 17’5 Mm of NaNO3.
- Autoclave at 121ºC during 20 minutes.
- Add 10 Mm of Hepes of NaOH to set the Ph=7’8 (1 M Hepes NaOH = 700 Ml distilled water, 283’3 g Hepes, ≈ 5’5 g NaOH).
