Team:TMU-Tokyo/Notebook/Experiment

From 2012.igem.org

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<Br>
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<B>■Experiment</B><Br>
<B>■Experiment</B><Br>
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<B>1st week (8.13 - 8.19)</A></B><Br>
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1st week (8.13 - 8.19)<Br>
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<B>2nd week (8.20 - 8.26)</A></B><Br>
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2nd week (8.20 - 8.26)<Br>
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<B>3rd week (8.27 - 9. 2)</A></B><Br>
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3rd week (8.27 - 9. 2)<Br>
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<B>4th week (9. 3 - 9. 9)</A></B><Br>
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4th week (9. 3 - 9. 9)<Br>
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<B>5th week (9.10 - 9.16)</A></B><Br>
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5th week (9.10 - 9.16)<Br>
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<B>6th week (9.17 - 9.23)</A></B><Br>
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6th week (9.17 - 9.23)<Br>
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<B>7th week (9.24 - 9.30)</A></B><Br>
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7th week (9.24 - 9.30)<Br>
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<B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Protocol/">■Protocols</A></B><Br>
<B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Protocol/">■Protocols</A></B><Br>
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Plasmid DNA Purification<Br>
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Genome DNA Purification<Br>
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Restruction Enzyme Degestion<Br>
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DNA Fragment Ligation<Br>
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Transformation<Br>
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Electrophoresis<Br>
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LB Medium<Br>
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<B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay">■Assay</A></B><Br>
<B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay">■Assay</A></B><Br>
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Assay0<Br>
Device1 Assay<Br>
Device1 Assay<Br>
Device2 Assay<Br>
Device2 Assay<Br>
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<div style="margin-left:20px">
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<p class="description3">
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<p class="description">
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<b>■Week 0(8.9-8.11)</b><Br>
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<b>■Week 0(8.9-8.11)</b><Br><p class="description3">
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<Br><b>■ 9th Aug</b><br>
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<b>■9th Aug</b><br>
Preparation of culture plates with Ampicillin<Br>
Preparation of culture plates with Ampicillin<Br>
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<b>■ 10th Aug</b><br>
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<b>■10th Aug</b><br>
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate<Br>
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate<Br>
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<b>■ 11th Aug</b><br>
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<b>■11th Aug</b><br>
Confirmation of the E.coli growth<Br>
Confirmation of the E.coli growth<Br>
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Cultivation of the E.coli on LB culture<Br></p>
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Cultivation of the E.coli on LB culture<Br>
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<Br>
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<b>■12th Aug</b><Br>
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Plasmid purification BBa_K299009<Br>
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</p>
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</div>
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Latest revision as of 02:32, 27 September 2012

 




■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols


■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay




Experiment



Experiment Overview


■Week 0(8.9-8.11)


■9th Aug
Preparation of culture plates with Ampicillin

■10th Aug
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate

■11th Aug
Confirmation of the E.coli growth
Cultivation of the E.coli on LB culture

■12th Aug
Plasmid purification BBa_K299009


■1st week (8.13 - 8.19)
Make Device3→more


■2nd week (8.20 - 8.26)
Make Device1→more


■3rd week (8.27 - 9. 2)
Make Device1 and Make Device3→more


■4th week (9. 3 - 9. 9)
Make all Device→more


■5th week (9.10 - 9.16)
Make all Device→more


■6th week (9.17 - 9.23)
Make all Device→more


■7th week (9.24 - 9.30)
Assay→more and Assay protocol