Team:TMU-Tokyo/Notebook/Experiment
From 2012.igem.org
(5 intermediate revisions not shown) | |||
Line 18: | Line 18: | ||
<Br> | <Br> | ||
<B>■Experiment</B><Br> | <B>■Experiment</B><Br> | ||
- | + | 1st week (8.13 - 8.19)<Br> | |
- | + | 2nd week (8.20 - 8.26)<Br> | |
- | + | 3rd week (8.27 - 9. 2)<Br> | |
- | + | 4th week (9. 3 - 9. 9)<Br> | |
- | + | 5th week (9.10 - 9.16)<Br> | |
- | + | 6th week (9.17 - 9.23)<Br> | |
- | + | 7th week (9.24 - 9.30)<Br> | |
<Br> | <Br> | ||
<Br> | <Br> | ||
<B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Protocol/">■Protocols</A></B><Br> | <B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Protocol/">■Protocols</A></B><Br> | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<Br> | <Br> | ||
<Br> | <Br> | ||
<B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay">■Assay</A></B><Br> | <B><A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay">■Assay</A></B><Br> | ||
+ | Assay0<Br> | ||
Device1 Assay<Br> | Device1 Assay<Br> | ||
Device2 Assay<Br> | Device2 Assay<Br> | ||
Line 57: | Line 52: | ||
<Hr><Br> | <Hr><Br> | ||
</p> | </p> | ||
- | <p class="description">Experiment Overview</p> | + | <p class="description"><b>Experiment Overview</b></p> |
<Br> | <Br> | ||
- | <p class=" | + | <div style="margin-left:20px"> |
- | <b>■Week 0(8.9-8.11)</b><Br> | + | <p class="description"> |
- | <Br><b> | + | <b>■Week 0(8.9-8.11)</b><Br><p class="description3"> |
+ | <Br> | ||
+ | <b>■9th Aug</b><br> | ||
Preparation of culture plates with Ampicillin<Br> | Preparation of culture plates with Ampicillin<Br> | ||
- | <b> | + | <Br> |
+ | <b>■10th Aug</b><br> | ||
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate<Br> | Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate<Br> | ||
- | <b> | + | <Br> |
+ | <b>■11th Aug</b><br> | ||
Confirmation of the E.coli growth<Br> | Confirmation of the E.coli growth<Br> | ||
- | Cultivation of the E.coli on LB culture<Br></p> | + | Cultivation of the E.coli on LB culture<Br> |
+ | <Br> | ||
+ | <b>■12th Aug</b><Br> | ||
+ | Plasmid purification BBa_K299009<Br> | ||
+ | <Br> | ||
+ | |||
+ | </p> | ||
<Br> | <Br> | ||
+ | </div> | ||
<p class="description"> | <p class="description"> | ||
Latest revision as of 02:32, 27 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay
Experiment
Experiment Overview
■Week 0(8.9-8.11)
■9th Aug
Preparation of culture plates with Ampicillin
■10th Aug
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate
■11th Aug
Confirmation of the E.coli growth
Cultivation of the E.coli on LB culture
■12th Aug
Plasmid purification BBa_K299009
■1st week (8.13 - 8.19)
Make Device3→more
■2nd week (8.20 - 8.26)
Make Device1→more
■3rd week (8.27 - 9. 2)
Make Device1 and Make Device3→more
■4th week (9. 3 - 9. 9)
Make all Device→more
■5th week (9.10 - 9.16)
Make all Device→more
■6th week (9.17 - 9.23)
Make all Device→more
■7th week (9.24 - 9.30)
Assay→more and Assay protocol