Team:University College London/Module 4/Characterisation

From 2012.igem.org

(Difference between revisions)

Latest revision as of 02:12, 27 September 2012

Characterisation

Starvation induction of E. coli transformed with this module will be done using minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3.0% and 5.0%). Quantification of the reporter output (GFP) from the samples taken will be measured using Tecan Zafire2 microplate detection system to quantify fluorescence intensity (485 nm for excitation, 520 nm for emission).

@@ Line 1: / Line 1: @@
-{{:Team:University_College_London/templates/head|coverpicture=training}}
+{{:Team:University_College_London/templates/headnocover}}<html><iframe width="990" height="500" src="http://www.plasticrepublic.org/wikianimations/module4char.html"></iframe></html>
-= Module 4: Buoyancy=
 {{:Team:University_College_London/templates/module4menu}}
 == Characterisation==
-In order to test the buoyancy of our cells, we will be determining their relative density. Cells will float or sink based on their density relative to the density of the solution surrounding them. Hence, by using different concentration of salt solutions, and observing the buoyancy of cells, a quantitative gauge of cell buoyancy can be determined.
+Starvation induction of ''E. coli'' transformed with this module will be done using minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3.0% and 5.0%). Quantification of the reporter output (GFP) from the samples taken will be measured using Tecan Zafire2 microplate detection system to quantify fluorescence intensity (485 nm for excitation, 520 nm for emission).
 {{:Team:University_College_London/templates/foot}}