"
Team:Paris Bettencourt/Achievements
From 2012.igem.org
(Difference between revisions)
Zmarinkovic (Talk | contribs) (→Achievements of all the different modules) |
|||
| Line 11: | Line 11: | ||
<td> | <td> | ||
'''Achievements :''' | '''Achievements :''' | ||
| + | |||
| + | '''Semantic containment''' | ||
* Construction and characterization of 2 biobricks : | * Construction and characterization of 2 biobricks : | ||
** [http://partsregistry.org/Part:BBa_K914000 K914000] : P<sub>Lac</sub>-supD-T : tRNA amber suppressor | ** [http://partsregistry.org/Part:BBa_K914000 K914000] : P<sub>Lac</sub>-supD-T : tRNA amber suppressor | ||
| Line 17: | Line 19: | ||
The first part (supD) is well characterized and works well. For the second parts, it turns out that this mutation is quite leaky, although it works in lab conditions, one mutation is not enough if we want to release such parts in nature. Other reasons emphasize this observation, notably the weakness of being at one mutation to recover the protein functionality. | The first part (supD) is well characterized and works well. For the second parts, it turns out that this mutation is quite leaky, although it works in lab conditions, one mutation is not enough if we want to release such parts in nature. Other reasons emphasize this observation, notably the weakness of being at one mutation to recover the protein functionality. | ||
* Creation of a new category in the part registry : [http://partsregistry.org/Biosafety Semantic containment]. The aim of this category is to let people improving each part by adding for instance other amber mutations to existing part to increase the containment. | * Creation of a new category in the part registry : [http://partsregistry.org/Biosafety Semantic containment]. The aim of this category is to let people improving each part by adding for instance other amber mutations to existing part to increase the containment. | ||
| - | |||
| - | |||
| - | |||
| - | + | '''Resctriction enzyme system''' | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | ''' | + | |
* Construction of 4 biobricks [https://2012.igem.org/Team:Paris_Bettencourt/Restriction_Enzyme#Design [Read more]]: | * Construction of 4 biobricks [https://2012.igem.org/Team:Paris_Bettencourt/Restriction_Enzyme#Design [Read more]]: | ||
** [http://partsregistry.org/Part:BBa_K914003 K914003]: L-rhamnose-inducible promoter | ** [http://partsregistry.org/Part:BBa_K914003 K914003]: L-rhamnose-inducible promoter | ||
| Line 37: | Line 32: | ||
** [http://partsregistry.org/Part:BBa_K175027 K175027]: I-SceI restriction site | ** [http://partsregistry.org/Part:BBa_K175027 K175027]: I-SceI restriction site | ||
* Currently we are in process of [https://2012.igem.org/Team:Paris_Bettencourt/Restriction_Enzyme#Design L-rhamnose-inducible promoter (pRha)] caracterisation. [https://2012.igem.org/Team:Paris_Bettencourt/Restriction_Enzyme#Characterisation_of_pRha [Read more]] | * Currently we are in process of [https://2012.igem.org/Team:Paris_Bettencourt/Restriction_Enzyme#Design L-rhamnose-inducible promoter (pRha)] caracterisation. [https://2012.igem.org/Team:Paris_Bettencourt/Restriction_Enzyme#Characterisation_of_pRha [Read more]] | ||
| - | |||
| - | |||
| + | '''Suicide system''' | ||
| - | |||
| - | |||
| - | |||
| - | |||
We showed that Colicin E2 cells induce cell death in sensitive populations, and that these sensitive populations can be protected by providing them with our engineered immunity protein. | We showed that Colicin E2 cells induce cell death in sensitive populations, and that these sensitive populations can be protected by providing them with our engineered immunity protein. | ||
* Construction of 2 biobricks : | * Construction of 2 biobricks : | ||
| Line 51: | Line 41: | ||
Part K914001 is well characterized and provides immunity to sensitive cells against the Colicin E2 activity protein, but is leaky. Part K914002 is promoterless and allows users to easily plug in the appropriate promoter for their desired purpose. | Part K914001 is well characterized and provides immunity to sensitive cells against the Colicin E2 activity protein, but is leaky. Part K914002 is promoterless and allows users to easily plug in the appropriate promoter for their desired purpose. | ||
* Creation of a new category in the part registry : [http://partsregistry.org/Biosafety XNase]. The aim of this category is to provide users with DNase/RNase parts that can be used for improved kill switches featuring the degradation of genomic material. | * Creation of a new category in the part registry : [http://partsregistry.org/Biosafety XNase]. The aim of this category is to provide users with DNase/RNase parts that can be used for improved kill switches featuring the degradation of genomic material. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| + | '''MAGE''' | ||
*Proof of concept by introducing a stop codon in the middle of the lacZ gene using multiplex automated genome engineering (MAGE) | *Proof of concept by introducing a stop codon in the middle of the lacZ gene using multiplex automated genome engineering (MAGE) | ||
| - | + | '''Synthetic Import Domain''' | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | ''' | + | |
| - | + | ||
*Construction of colicin-like toxin by fusing Colicin E2 based "Synthetic Import Domain" with RNAse domain of colicin D | *Construction of colicin-like toxin by fusing Colicin E2 based "Synthetic Import Domain" with RNAse domain of colicin D | ||
*Constructon of FseI, I-SceI, LuxR active fragment, LacZ alpha fragment, PyrF and T7 RNA polymerase fused to the two types of "Synthetic Import Domains" from Colicin E2 and Colicin D | *Constructon of FseI, I-SceI, LuxR active fragment, LacZ alpha fragment, PyrF and T7 RNA polymerase fused to the two types of "Synthetic Import Domains" from Colicin E2 and Colicin D | ||
Revision as of 01:15, 27 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety Assessment
Achievements
Achievements of all the different modules
|
Achievements : Semantic containment
The first part (supD) is well characterized and works well. For the second parts, it turns out that this mutation is quite leaky, although it works in lab conditions, one mutation is not enough if we want to release such parts in nature. Other reasons emphasize this observation, notably the weakness of being at one mutation to recover the protein functionality.
Resctriction enzyme system
Suicide system We showed that Colicin E2 cells induce cell death in sensitive populations, and that these sensitive populations can be protected by providing them with our engineered immunity protein.
Part K914001 is well characterized and provides immunity to sensitive cells against the Colicin E2 activity protein, but is leaky. Part K914002 is promoterless and allows users to easily plug in the appropriate promoter for their desired purpose.
MAGE
Synthetic Import Domain
|
