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Team:University College London/HumanPractice/DIYbio/Workshops
From 2012.igem.org
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== Result: Analytical Digest of the Public Biobrick == | == Result: Analytical Digest of the Public Biobrick == | ||
[[File:Analytical_digest_public_biobrick.jpg]] | [[File:Analytical_digest_public_biobrick.jpg]] | ||
| - | <div class="">''''''</div> Five colonies were picked from the ligation plates. After inoculation in LB + chloramphenicol overnight, these were minipreped and analytical digest was performed. All samples in lanes labelled one were digested once with Eco RI and samples labelled two are digested with EcoRI and Pst I. Colonies C and D clearly have the antifreeze insert. Lanes C2 and D2 show bands around 2000bp for the CMP plasmid backbone and around 400bp for the 435bp antifreeze region amplified by the primers. | + | <div class="">''''''</div> Five colonies were picked from the ligation plates. After inoculation in LB + chloramphenicol overnight, these were minipreped and analytical digest was performed. All samples in lanes labelled one were digested once with Eco RI and samples labelled two are digested with EcoRI and Pst I. Colonies C and D clearly have the antifreeze insert. Lanes C2 and D2 show bands around 2000bp for the CMP plasmid backbone and around 400bp for the 435bp antifreeze region amplified by the primers. Therefore we have the biobrick! |
== Safety and regulations == | == Safety and regulations == | ||
Revision as of 21:22, 26 September 2012
Contents |
DIYbio
Overview | Concept | DIYbio | Workshops | Exhibition | Evaluation | Conclusion
Planning
Hackspace Workshop schedule
In the beginning of September, we held three day workshops in UCL teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the biohackers if the pace was slowed down. The amount of practical experience between these citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the biohackers questions and to show the biohackers proper techniques we use in the laboratory.
Original UCL Wetlab Workshop schedule
Result: Analytical Digest of the Public Biobrick
Safety and regulations
Legality of making competent cell protocol at London Hackspace
